mirror of
https://github.com/MillironX/XAM.jl.git
synced 2024-12-24 13:48:19 +00:00
Documentation
- API autodocs. - Inline links for documenter. - Logo. - Badges.
This commit is contained in:
parent
30a9911e58
commit
1bcd7c344c
7 changed files with 260 additions and 28 deletions
77
README.md
77
README.md
|
@ -1,3 +1,76 @@
|
||||||
# XAM.jl
|
# <img src="./docs/src/assets/logo.svg" width="30%" align="right" /> XAM.jl
|
||||||
|
|
||||||
In development: minimal working separation of SAM and BAM from BioAlignments.
|
[![Project Status: WIP – Initial development is in progress, but there has not yet been a stable, usable release suitable for the public.](https://www.repostatus.org/badges/latest/wip.svg)](https://www.repostatus.org/#wip)
|
||||||
|
[![Latest Release](https://img.shields.io/github/release/BioJulia/XAM.jl.svg)](https://github.com/BioJulia/XAM.jl/releases/latest)
|
||||||
|
[![MIT license](https://img.shields.io/badge/license-MIT-green.svg)](https://github.com/BioJulia/XAM.jl/blob/master/LICENSE)
|
||||||
|
[![Stable documentation](https://img.shields.io/badge/docs-stable-blue.svg)](https://biojulia.github.io/XAM.jl/stable)
|
||||||
|
[![Latest documentation](https://img.shields.io/badge/docs-dev-blue.svg)](https://biojulia.github.io/XAM.jl/dev/)
|
||||||
|
[![Join the chat at https://gitter.im/BioJulia/XAM.jl](https://badges.gitter.im/BioJulia/XAM.jl.svg)](https://gitter.im/BioJulia/XAM.jl)
|
||||||
|
|
||||||
|
> This project follows the [semver](http://semver.org) pro forma and uses the [git-flow branching model](https://nvie.com/posts/a-successful-git-branching-model/ "original
|
||||||
|
blog post").
|
||||||
|
|
||||||
|
## Description
|
||||||
|
XAM provides I/O and utilities for manipulating SAM and BAM formatted alignment map files.
|
||||||
|
|
||||||
|
## Installation
|
||||||
|
The latest version of XAM is made available to install through BioJulia's package registry.
|
||||||
|
By default, Julia's package manager only includes the "General" package registry.
|
||||||
|
|
||||||
|
To add the BioJulia registry from the [Julia REPL](https://docs.julialang.org/en/v1/manual/getting-started/), press `]` to enter [pkg mode](https://docs.julialang.org/en/v1/stdlib/Pkg/), then enter the following command:
|
||||||
|
```julia
|
||||||
|
registry add https://github.com/BioJulia/BioJuliaRegistry.git
|
||||||
|
```
|
||||||
|
|
||||||
|
Once the registry is added to your configuration, you can install XAM while in [pkg mode](https://docs.julialang.org/en/v1/stdlib/Pkg/) with the following:
|
||||||
|
```julia
|
||||||
|
add XAM
|
||||||
|
```
|
||||||
|
|
||||||
|
If you are interested in the cutting edge of the development, please check out the [develop branch](https://github.com/BioJulia/XAM.jl/tree/develop) to try new features before release.
|
||||||
|
|
||||||
|
## Testing
|
||||||
|
XAM is tested against Julia `1.X` on Linux, OS X, and Windows.
|
||||||
|
|
||||||
|
**Latest build status:**
|
||||||
|
|
||||||
|
[![Unit tests](https://github.com/BioJulia/XAM.jl/workflows/Unit%20tests/badge.svg?branch=master)](https://github.com/BioJulia/XAM.jl/actions?query=workflow%3A%22Unit+tests%22+branch%3Amaster)
|
||||||
|
[![Documentation](https://github.com/BioJulia/XAM.jl/workflows/Documentation/badge.svg?branch=master)](https://github.com/BioJulia/XAM.jl/actions?query=workflow%3ADocumentation+branch%3Amaster)
|
||||||
|
[![codecov](https://codecov.io/gh/BioJulia/XAM.jl/branch/master/graph/badge.svg)](https://codecov.io/gh/BioJulia/XAM.jl)
|
||||||
|
|
||||||
|
## Contributing
|
||||||
|
We appreciate [contributions](https://github.com/BioJulia/XAM.jl/graphs/contributors) from users including reporting bugs, fixing issues, improving performance and adding new features.
|
||||||
|
|
||||||
|
Take a look at the [contributing files](https://github.com/BioJulia/Contributing) detailed contributor and maintainer guidelines, and code of conduct.
|
||||||
|
|
||||||
|
### Financial contributions
|
||||||
|
We also welcome financial contributions in full transparency on our [open collective](https://opencollective.com/biojulia).
|
||||||
|
Anyone can file an expense.
|
||||||
|
If the expense makes sense for the development the core contributors and the person who filed the expense will be reimbursed.
|
||||||
|
|
||||||
|
|
||||||
|
## Backers & Sponsors
|
||||||
|
Thank you to all our backers and sponsors!
|
||||||
|
|
||||||
|
Love our work and community? [Become a backer](https://opencollective.com/biojulia#backer).
|
||||||
|
|
||||||
|
[![backers](https://opencollective.com/biojulia/backers.svg?width=890)](https://opencollective.com/biojulia#backers)
|
||||||
|
|
||||||
|
Does your company use BioJulia?
|
||||||
|
Help keep BioJulia feature rich and healthy by [sponsoring the project](https://opencollective.com/biojulia#sponsor).
|
||||||
|
Your logo will show up here with a link to your website.
|
||||||
|
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/0/avatar.svg)](https://opencollective.com/biojulia/sponsor/0/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/1/avatar.svg)](https://opencollective.com/biojulia/sponsor/1/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/2/avatar.svg)](https://opencollective.com/biojulia/sponsor/2/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/3/avatar.svg)](https://opencollective.com/biojulia/sponsor/3/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/4/avatar.svg)](https://opencollective.com/biojulia/sponsor/4/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/5/avatar.svg)](https://opencollective.com/biojulia/sponsor/5/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/6/avatar.svg)](https://opencollective.com/biojulia/sponsor/6/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/7/avatar.svg)](https://opencollective.com/biojulia/sponsor/7/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/8/avatar.svg)](https://opencollective.com/biojulia/sponsor/8/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/9/avatar.svg)](https://opencollective.com/biojulia/sponsor/9/website)
|
||||||
|
|
||||||
|
|
||||||
|
## Questions?
|
||||||
|
If you have a question about contributing or using BioJulia software, come on over and chat to us on [Gitter](https://gitter.im/BioJulia/General), or you can try the [Bio category of the Julia discourse site](https://discourse.julialang.org/c/domain/bio).
|
||||||
|
|
6
docs/Project.toml
Normal file
6
docs/Project.toml
Normal file
|
@ -0,0 +1,6 @@
|
||||||
|
[deps]
|
||||||
|
Documenter = "e30172f5-a6a5-5a46-863b-614d45cd2de4"
|
||||||
|
Pkg = "44cfe95a-1eb2-52ea-b672-e2afdf69b78f"
|
||||||
|
|
||||||
|
[compat]
|
||||||
|
Documenter = "0.24"
|
21
docs/make.jl
Normal file
21
docs/make.jl
Normal file
|
@ -0,0 +1,21 @@
|
||||||
|
using Pkg
|
||||||
|
using Documenter, XAM
|
||||||
|
|
||||||
|
makedocs(
|
||||||
|
format = Documenter.HTML(
|
||||||
|
edit_link = "develop"
|
||||||
|
),
|
||||||
|
modules = [XAM, XAM.SAM, XAM.BAM],
|
||||||
|
sitename = "XAM.jl",
|
||||||
|
pages = [
|
||||||
|
"Home" => "index.md",
|
||||||
|
"SAM and BAM" => "man/hts-files.md",
|
||||||
|
"API Reference" => "man/api.md"
|
||||||
|
],
|
||||||
|
authors = replace(join(Pkg.TOML.parsefile("Project.toml")["authors"], ", "), r" <.*?>" => "" ) * ", The BioJulia Organisation, and other contributors."
|
||||||
|
)
|
||||||
|
deploydocs(
|
||||||
|
repo = "github.com/BioJulia/XAM.jl.git",
|
||||||
|
devbranch = "develop",
|
||||||
|
push_preview = true
|
||||||
|
)
|
26
docs/src/assets/logo.svg
Normal file
26
docs/src/assets/logo.svg
Normal file
|
@ -0,0 +1,26 @@
|
||||||
|
<svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 136.48101 51.472999">
|
||||||
|
<g id="bubble-helix">
|
||||||
|
<circle cx="5.9792" cy="20.3" r="5.9792" fill="#945bb0"/>
|
||||||
|
<circle cx="21.317" cy="39.5" r="4.9393" fill="#4266d5"/>
|
||||||
|
<circle cx="16.378" cy="11.7" r="4.9393" fill="#c93d39"/>
|
||||||
|
<circle cx="35.095" cy="35.9" r="5.9792" fill="#945bb0"/>
|
||||||
|
<circle cx="47.184" cy="23.8" r="7.409" fill="#4266d5"/>
|
||||||
|
<circle cx="28.986" cy="9.7" r="4.5494" fill="#3b972e"/>
|
||||||
|
<circle cx="89.428" cy="46.5" r="4.9393" fill="#c93d39"/>
|
||||||
|
<circle cx="71.62" cy="5.6" r="5.5892" fill="#945bb0"/>
|
||||||
|
<circle cx="121.01" cy="15.5" r="5.5892" fill="#c93d39"/>
|
||||||
|
<circle cx="59.662" cy="30.3" r="2.4697" fill="#4266d5"/>
|
||||||
|
<circle cx="103.6" cy="19.9" r="3.2496" fill="#c93d39"/>
|
||||||
|
<circle cx="123.61" cy="36.5" r="2.7296" fill="#4266d5"/>
|
||||||
|
<circle cx="127.64" cy="5.2" r="3.8995" fill="#3b972e"/>
|
||||||
|
<circle cx="132.58" cy="39.2" r="3.8995" fill="#945bb0"/>
|
||||||
|
<circle cx="67.591" cy="37.4" r="3.3795" fill="#3b972e"/>
|
||||||
|
<circle cx="95.017" cy="13.9" r="4.2894" fill="#4266d5"/>
|
||||||
|
<circle cx="77.729" cy="42.9" r="3.8995" fill="#945bb0"/>
|
||||||
|
<circle cx="102.56" cy="42.0" r="5.8492" fill="#4266d5"/>
|
||||||
|
<circle cx="113.6" cy="28.8" r="6.7591" fill="#3b972e"/>
|
||||||
|
<circle cx="57.582" cy="11.0" r="6.3691" fill="#c93d39"/>
|
||||||
|
<circle cx="38.735" cy="14.8" r="3.3795" fill="#c93d39"/>
|
||||||
|
<circle cx="84.618" cy="7.9" r="5.3293" fill="#3b972e"/>
|
||||||
|
</g>
|
||||||
|
</svg>
|
After Width: | Height: | Size: 1.4 KiB |
77
docs/src/index.md
Normal file
77
docs/src/index.md
Normal file
|
@ -0,0 +1,77 @@
|
||||||
|
# XAM.jl
|
||||||
|
|
||||||
|
[![Project Status: WIP – Initial development is in progress, but there has not yet been a stable, usable release suitable for the public.](https://www.repostatus.org/badges/latest/wip.svg)](https://www.repostatus.org/#wip)
|
||||||
|
[![Latest Release](https://img.shields.io/github/release/BioJulia/XAM.jl.svg)](https://github.com/BioJulia/XAM.jl/releases/latest)
|
||||||
|
[![MIT license](https://img.shields.io/badge/license-MIT-green.svg)](https://github.com/BioJulia/XAM.jl/blob/master/LICENSE)
|
||||||
|
[![Stable documentation](https://img.shields.io/badge/docs-stable-blue.svg)](https://biojulia.github.io/XAM.jl/stable)
|
||||||
|
[![Latest documentation](https://img.shields.io/badge/docs-dev-blue.svg)](https://biojulia.github.io/XAM.jl/dev/)
|
||||||
|
[![Join the chat at https://gitter.im/BioJulia/XAM.jl](https://badges.gitter.im/BioJulia/XAM.jl.svg)](https://gitter.im/BioJulia/XAM.jl)
|
||||||
|
|
||||||
|
> This project follows the [semver](http://semver.org) pro forma and uses the [git-flow branching model](https://nvie.com/posts/a-successful-git-branching-model/).
|
||||||
|
|
||||||
|
## Description
|
||||||
|
|
||||||
|
XAM provides I/O and utilities for manipulating SAM and BAM formatted alignment map files.
|
||||||
|
|
||||||
|
|
||||||
|
## Installation
|
||||||
|
The latest version of XAM is made available to install through BioJulia's package registry.
|
||||||
|
By default, Julia's package manager only includes the "General" package registry.
|
||||||
|
|
||||||
|
To add the BioJulia registry from the [Julia REPL](https://docs.julialang.org/en/v1/manual/getting-started/), press `]` to enter [pkg mode](https://docs.julialang.org/en/v1/stdlib/Pkg/), then enter the following command:
|
||||||
|
```julia
|
||||||
|
registry add https://github.com/BioJulia/BioJuliaRegistry.git
|
||||||
|
```
|
||||||
|
|
||||||
|
Once the registry is added to your configuration, you can install XAM while in [pkg mode](https://docs.julialang.org/en/v1/stdlib/Pkg/) with the following:
|
||||||
|
```julia
|
||||||
|
add XAM
|
||||||
|
```
|
||||||
|
|
||||||
|
If you are interested in the cutting edge of the development, please check out the [develop branch](https://github.com/BioJulia/XAM.jl/tree/develop) to try new features before release.
|
||||||
|
|
||||||
|
## Testing
|
||||||
|
XAM is tested against Julia `1.X` on Linux, OS X, and Windows.
|
||||||
|
|
||||||
|
**Latest build status:**
|
||||||
|
|
||||||
|
[![Unit tests](https://github.com/BioJulia/XAM.jl/workflows/Unit%20tests/badge.svg?branch=master)](https://github.com/BioJulia/XAM.jl/actions?query=workflow%3A%22Unit+tests%22+branch%3Amaster)
|
||||||
|
[![Documentation](https://github.com/BioJulia/XAM.jl/workflows/Documentation/badge.svg?branch=master)](https://github.com/BioJulia/XAM.jl/actions?query=workflow%3ADocumentation+branch%3Amaster)
|
||||||
|
[![codecov](https://codecov.io/gh/BioJulia/XAM.jl/branch/master/graph/badge.svg)](https://codecov.io/gh/BioJulia/XAM.jl)
|
||||||
|
|
||||||
|
## Contributing
|
||||||
|
We appreciate [contributions](https://github.com/BioJulia/XAM.jl/graphs/contributors) from users including reporting bugs, fixing issues, improving performance and adding new features.
|
||||||
|
|
||||||
|
Take a look at the [contributing files](https://github.com/BioJulia/Contributing) detailed contributor and maintainer guidelines, and code of conduct.
|
||||||
|
|
||||||
|
### Financial contributions
|
||||||
|
We also welcome financial contributions in full transparency on our [open collective](https://opencollective.com/biojulia).
|
||||||
|
Anyone can file an expense.
|
||||||
|
If the expense makes sense for the development the core contributors and the person who filed the expense will be reimbursed.
|
||||||
|
|
||||||
|
|
||||||
|
## Backers & Sponsors
|
||||||
|
Thank you to all our backers and sponsors!
|
||||||
|
|
||||||
|
Love our work and community? [Become a backer](https://opencollective.com/biojulia#backer).
|
||||||
|
|
||||||
|
[![backers](https://opencollective.com/biojulia/backers.svg?width=890)](https://opencollective.com/biojulia#backers)
|
||||||
|
|
||||||
|
Does your company use BioJulia?
|
||||||
|
Help keep BioJulia feature rich and healthy by [sponsoring the project](https://opencollective.com/biojulia#sponsor).
|
||||||
|
Your logo will show up here with a link to your website.
|
||||||
|
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/0/avatar.svg)](https://opencollective.com/biojulia/sponsor/0/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/1/avatar.svg)](https://opencollective.com/biojulia/sponsor/1/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/2/avatar.svg)](https://opencollective.com/biojulia/sponsor/2/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/3/avatar.svg)](https://opencollective.com/biojulia/sponsor/3/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/4/avatar.svg)](https://opencollective.com/biojulia/sponsor/4/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/5/avatar.svg)](https://opencollective.com/biojulia/sponsor/5/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/6/avatar.svg)](https://opencollective.com/biojulia/sponsor/6/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/7/avatar.svg)](https://opencollective.com/biojulia/sponsor/7/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/8/avatar.svg)](https://opencollective.com/biojulia/sponsor/8/website)
|
||||||
|
[![](https://opencollective.com/biojulia/sponsor/9/avatar.svg)](https://opencollective.com/biojulia/sponsor/9/website)
|
||||||
|
|
||||||
|
|
||||||
|
## Questions?
|
||||||
|
If you have a question about contributing or using BioJulia software, come on over and chat to us on [Gitter](https://gitter.im/BioJulia/General), or you can try the [Bio category of the Julia discourse site](https://discourse.julialang.org/c/domain/bio).
|
38
docs/src/man/api.md
Normal file
38
docs/src/man/api.md
Normal file
|
@ -0,0 +1,38 @@
|
||||||
|
```@meta
|
||||||
|
CurrentModule = XAM
|
||||||
|
DocTestSetup = quote
|
||||||
|
using XAM
|
||||||
|
end
|
||||||
|
```
|
||||||
|
|
||||||
|
# API Reference
|
||||||
|
|
||||||
|
## SAM API
|
||||||
|
|
||||||
|
### Public
|
||||||
|
|
||||||
|
```@autodocs
|
||||||
|
Modules = [XAM.SAM]
|
||||||
|
private = false
|
||||||
|
```
|
||||||
|
|
||||||
|
### Internal
|
||||||
|
```@autodocs
|
||||||
|
Modules = [XAM.SAM]
|
||||||
|
public = false
|
||||||
|
```
|
||||||
|
|
||||||
|
## BAM API
|
||||||
|
|
||||||
|
### Public
|
||||||
|
|
||||||
|
```@autodocs
|
||||||
|
Modules = [XAM.BAM]
|
||||||
|
private = false
|
||||||
|
```
|
||||||
|
|
||||||
|
### Internal
|
||||||
|
```@autodocs
|
||||||
|
Modules = [XAM.BAM]
|
||||||
|
public = false
|
||||||
|
```
|
|
@ -1,15 +1,10 @@
|
||||||
# SAM and BAM
|
# SAM and BAM
|
||||||
|
|
||||||
|
|
||||||
## Introduction
|
## Introduction
|
||||||
|
|
||||||
High-throughput sequencing (HTS) technologies generate a large amount of data in the form of a large number of nucleotide sequencing reads.
|
The `XAM` package offers high-performance tools for SAM and BAM file formats, which are the most popular file formats.
|
||||||
One of the most common tasks in bioinformatics is to align these reads against known reference genomes, chromosomes, or contigs.
|
|
||||||
BioAlignments provides several data formats commonly used for this kind of task.
|
|
||||||
|
|
||||||
BioAlignments offers high-performance tools for SAM and BAM file formats, which are the most popular file formats.
|
If you have questions about the SAM and BAM formats or any of the terminology used when discussing these formats, see the published [specification](https://samtools.github.io/hts-specs/SAMv1.pdf), which is maintained by the [samtools group](https://samtools.github.io/).
|
||||||
|
|
||||||
If you have questions about the SAM and BAM formats or any of the terminology used when discussing these formats, see the published [specification][samtools-spec], which is maintained by the [samtools group][samtools].
|
|
||||||
|
|
||||||
A very very simple SAM file looks like the following:
|
A very very simple SAM file looks like the following:
|
||||||
|
|
||||||
|
@ -28,7 +23,7 @@ Where the first two lines are part of the "header", and the following lines are
|
||||||
Each record describes how a read aligns to some reference sequence.
|
Each record describes how a read aligns to some reference sequence.
|
||||||
Sometimes one record describes one read, but there are other cases like chimeric reads and split alignments, where multiple records apply to one read.
|
Sometimes one record describes one read, but there are other cases like chimeric reads and split alignments, where multiple records apply to one read.
|
||||||
In the example above, `r003` is a chimeric read, and `r004` is a split alignment, and `r001` are mate pair reads.
|
In the example above, `r003` is a chimeric read, and `r004` is a split alignment, and `r001` are mate pair reads.
|
||||||
Again, we refer you to the official [specification][samtools-spec] for more details.
|
Again, we refer you to the official [specification](https://samtools.github.io/hts-specs/SAMv1.pdf) for more details.
|
||||||
|
|
||||||
A BAM file stores this same information but in a binary and compressible format that does not make for pretty printing here!
|
A BAM file stores this same information but in a binary and compressible format that does not make for pretty printing here!
|
||||||
|
|
||||||
|
@ -37,7 +32,7 @@ A BAM file stores this same information but in a binary and compressible format
|
||||||
A typical script iterating over all records in a file looks like below:
|
A typical script iterating over all records in a file looks like below:
|
||||||
|
|
||||||
```julia
|
```julia
|
||||||
using BioAlignments
|
using XAM
|
||||||
|
|
||||||
# Open a BAM file.
|
# Open a BAM file.
|
||||||
reader = open(BAM.Reader, "data.bam")
|
reader = open(BAM.Reader, "data.bam")
|
||||||
|
@ -72,7 +67,7 @@ end
|
||||||
|
|
||||||
Both `SAM.Reader` and `BAM.Reader` implement the `header` function, which returns a `SAM.Header` object.
|
Both `SAM.Reader` and `BAM.Reader` implement the `header` function, which returns a `SAM.Header` object.
|
||||||
To extract certain information out of the headers, you can use the `find` method on the header to extract information according to SAM/BAM tag.
|
To extract certain information out of the headers, you can use the `find` method on the header to extract information according to SAM/BAM tag.
|
||||||
Again we refer you to the [specification][samtools-spec] for full details of all the different tags that can occur in headers, and what they mean.
|
Again we refer you to the [specification](https://samtools.github.io/hts-specs/SAMv1.pdf) for full details of all the different tags that can occur in headers, and what they mean.
|
||||||
|
|
||||||
Below is an example of extracting all the info about the reference sequences from the BAM header.
|
Below is an example of extracting all the info about the reference sequences from the BAM header.
|
||||||
In SAM/BAM, any description of a reference sequence is stored in the header, under a tag denoted `SQ` (think `reference SeQuence`!).
|
In SAM/BAM, any description of a reference sequence is stored in the header, under a tag denoted `SQ` (think `reference SeQuence`!).
|
||||||
|
@ -110,7 +105,8 @@ In the above we can see there were 7 sequences in the reference: 5 chromosomes,
|
||||||
|
|
||||||
## SAM and BAM Records
|
## SAM and BAM Records
|
||||||
|
|
||||||
BioAlignments supports the following accessors for `SAM.Record` types.
|
### SAM.Record
|
||||||
|
The `XAM` package supports the following accessors for `SAM.Record` types.
|
||||||
|
|
||||||
```@docs
|
```@docs
|
||||||
XAM.SAM.flag
|
XAM.SAM.flag
|
||||||
|
@ -134,7 +130,8 @@ XAM.SAM.quality
|
||||||
XAM.SAM.auxdata
|
XAM.SAM.auxdata
|
||||||
```
|
```
|
||||||
|
|
||||||
BioAlignments supports the following accessors for `BAM.Record` types.
|
### BAM.Record
|
||||||
|
The `XAM` package supports the following accessors for `BAM.Record` types.
|
||||||
|
|
||||||
```@docs
|
```@docs
|
||||||
XAM.BAM.flag
|
XAM.BAM.flag
|
||||||
|
@ -178,7 +175,7 @@ Tagged auxiliary data follows a format of `TAG:TYPE:VALUE`.
|
||||||
| 'H' | Byte array in Hex format |
|
| 'H' | Byte array in Hex format |
|
||||||
| 'B' | Integer of numeric array |
|
| 'B' | Integer of numeric array |
|
||||||
|
|
||||||
For more information about these tags and their types we refer you to the [SAM/BAM specification][samtools-spec] and the additional [optional fields specification][samtags] document.
|
For more information about these tags and their types we refer you to the [SAM/BAM specification](https://samtools.github.io/hts-specs/SAMv1.pdf) and the additional [optional fields specification](https://samtools.github.io/hts-specs/SAMtags.pdf) document.
|
||||||
|
|
||||||
There are some tags that are reserved, predefined standard tags, for specific uses.
|
There are some tags that are reserved, predefined standard tags, for specific uses.
|
||||||
|
|
||||||
|
@ -200,8 +197,8 @@ end
|
||||||
|
|
||||||
## Getting records in a range
|
## Getting records in a range
|
||||||
|
|
||||||
BioAlignments supports the BAI index to fetch records in a specific range from a BAM file.
|
The `XAM` package supports the BAI index to fetch records in a specific range from a BAM file.
|
||||||
[Samtools][samtools] provides `index` subcommand to create an index file (.bai) from a sorted BAM file.
|
[Samtools](https://samtools.github.io/) provides `index` subcommand to create an index file (.bai) from a sorted BAM file.
|
||||||
|
|
||||||
```console
|
```console
|
||||||
$ samtools index -b SRR1238088.sort.bam
|
$ samtools index -b SRR1238088.sort.bam
|
||||||
|
@ -209,7 +206,7 @@ $ ls SRR1238088.sort.bam*
|
||||||
SRR1238088.sort.bam SRR1238088.sort.bam.bai
|
SRR1238088.sort.bam SRR1238088.sort.bam.bai
|
||||||
```
|
```
|
||||||
|
|
||||||
`eachoverlap(reader, chrom, range)` returns an iterator of BAM records overlapping the query interval:
|
The method `eachoverlap(reader, chrom, range)` returns an iterator of BAM records overlapping the query interval:
|
||||||
|
|
||||||
```julia
|
```julia
|
||||||
reader = open(BAM.Reader, "SRR1238088.sort.bam", index="SRR1238088.sort.bam.bai")
|
reader = open(BAM.Reader, "SRR1238088.sort.bam", index="SRR1238088.sort.bam.bai")
|
||||||
|
@ -222,14 +219,14 @@ close(reader)
|
||||||
|
|
||||||
## Getting records overlapping genomic features
|
## Getting records overlapping genomic features
|
||||||
|
|
||||||
`eachoverlap` also accepts the `Interval` type defined in [GenomicFeatures.jl][genomicfeatures].
|
The `eachoverlap` method also accepts the `Interval` type defined in [GenomicFeatures.jl](https://github.com/BioJulia/GenomicFeatures.jl).
|
||||||
|
|
||||||
This allows you to do things like first read in the genomic features from a GFF3 file, and then for each feature, iterate over all the BAM records that overlap with that feature.
|
This allows you to do things like first read in the genomic features from a GFF3 file, and then for each feature, iterate over all the BAM records that overlap with that feature.
|
||||||
|
|
||||||
```julia
|
```julia
|
||||||
# Load GFF3 module.
|
|
||||||
using GenomicFeatures
|
using GenomicFeatures
|
||||||
using BioAlignments
|
using GFF3
|
||||||
|
using XAM
|
||||||
|
|
||||||
# Load genomic features from a GFF3 file.
|
# Load genomic features from a GFF3 file.
|
||||||
features = open(collect, GFF3.Reader, "TAIR10_GFF3_genes.gff")
|
features = open(collect, GFF3.Reader, "TAIR10_GFF3_genes.gff")
|
||||||
|
@ -289,7 +286,7 @@ SAM.Writer(IOStream(<file my-data.sam>))
|
||||||
|
|
||||||
```
|
```
|
||||||
|
|
||||||
To make a BAM Writer is slightly different, as you need to use a specific stream type from the [BGZFStreams][bgzfstreams] package:
|
To make a BAM Writer is slightly different, as you need to use a specific stream type from the (https://github.com/BioJulia/BGZFStreams.jl)[https://github.com/BioJulia/BGZFStreams.jl] package:
|
||||||
|
|
||||||
```julia
|
```julia
|
||||||
julia> using BGZFStreams
|
julia> using BGZFStreams
|
||||||
|
@ -305,9 +302,3 @@ Once you have a BAM or SAM writer, you can use the `write` method to write `BAM.
|
||||||
julia> write(bamw, rec) # Here rec is a `BAM.Record`
|
julia> write(bamw, rec) # Here rec is a `BAM.Record`
|
||||||
330780
|
330780
|
||||||
```
|
```
|
||||||
|
|
||||||
[samtools]: https://samtools.github.io/
|
|
||||||
[samtools-spec]: https://samtools.github.io/hts-specs/SAMv1.pdf
|
|
||||||
[samtags]: https://samtools.github.io/hts-specs/SAMtags.pdf
|
|
||||||
[bgzfstreams]: https://github.com/BioJulia/BGZFStreams.jl
|
|
||||||
[genomicfeatures]: https://github.com/BioJulia/GenomicFeatures.jl
|
|
||||||
|
|
Loading…
Reference in a new issue