mirror of
https://github.com/MillironX/XAM.jl.git
synced 2024-11-14 22:33:14 +00:00
Merge pull request #29 from CiaranOMara/feature/crosscheck
Begin crosschecks
This commit is contained in:
commit
8836b4dbb6
4 changed files with 73 additions and 13 deletions
|
@ -511,9 +511,12 @@ end
|
|||
|
||||
Get the segment sequence of `record`.
|
||||
"""
|
||||
function sequence(record::Record)::BioSequences.LongDNASeq
|
||||
function sequence(record::Record)
|
||||
checkfilled(record)
|
||||
seqlen = seqlength(record)
|
||||
if seqlen == 0
|
||||
return nothing
|
||||
end
|
||||
data = Vector{UInt64}(undef, cld(seqlen, 16))
|
||||
src::Ptr{UInt64} = pointer(record.data, seqname_length(record) + n_cigar_op(record, false) * 4 + 1)
|
||||
for i in 1:lastindex(data)
|
||||
|
|
|
@ -206,9 +206,9 @@ Get the 1-based leftmost mapping position of `record`.
|
|||
function position(record::Record)::Int
|
||||
checkfilled(record)
|
||||
pos = unsafe_parse_decimal(Int, record.data, record.pos)
|
||||
if pos == 0
|
||||
missingerror(:position)
|
||||
end
|
||||
# if pos == 0
|
||||
# missingerror(:position)
|
||||
# end
|
||||
return pos
|
||||
end
|
||||
|
||||
|
@ -263,9 +263,9 @@ Get the position of the mate/next read of `record`.
|
|||
function nextposition(record::Record)::Int
|
||||
checkfilled(record)
|
||||
pos = unsafe_parse_decimal(Int, record.data, record.pnext)
|
||||
if pos == 0
|
||||
missingerror(:nextposition)
|
||||
end
|
||||
# if pos == 0
|
||||
# missingerror(:nextposition)
|
||||
# end
|
||||
return pos
|
||||
end
|
||||
|
||||
|
@ -299,7 +299,8 @@ Get the CIGAR string of `record`.
|
|||
function cigar(record::Record)::String
|
||||
checkfilled(record)
|
||||
if ismissing(record, record.cigar)
|
||||
missingerror(:cigar)
|
||||
# missingerror(:cigar)
|
||||
return ""
|
||||
end
|
||||
return String(record.data[record.cigar])
|
||||
end
|
||||
|
@ -377,9 +378,9 @@ Get the template length of `record`.
|
|||
function templength(record::Record)::Int
|
||||
checkfilled(record)
|
||||
len = unsafe_parse_decimal(Int, record.data, record.tlen)
|
||||
if len == 0
|
||||
missingerror(:tlen)
|
||||
end
|
||||
# if len == 0
|
||||
# missingerror(:tlen)
|
||||
# end
|
||||
return len
|
||||
end
|
||||
|
||||
|
@ -392,10 +393,11 @@ end
|
|||
|
||||
Get the segment sequence of `record`.
|
||||
"""
|
||||
function sequence(record::Record)::BioSequences.LongDNASeq
|
||||
function sequence(record::Record)
|
||||
checkfilled(record)
|
||||
if ismissing(record, record.seq)
|
||||
missingerror(:sequence)
|
||||
# missingerror(:sequence)
|
||||
return nothing
|
||||
end
|
||||
seqlen = length(record.seq)
|
||||
ret = BioSequences.LongDNASeq(seqlen)
|
||||
|
|
|
@ -25,3 +25,4 @@ end
|
|||
|
||||
include("test_sam.jl")
|
||||
include("test_bam.jl")
|
||||
include("test_crosscheck.jl")
|
||||
|
|
54
test/test_crosscheck.jl
Normal file
54
test/test_crosscheck.jl
Normal file
|
@ -0,0 +1,54 @@
|
|||
@testset "Cross Check Properties" begin
|
||||
|
||||
Broadcast.broadcastable(x::XAM.BAM.Record) = Ref(x) #TODO: consider moving to XAM.jl.
|
||||
Broadcast.broadcastable(x::XAM.SAM.Record) = Ref(x) #TODO: consider moving to XAM.jl
|
||||
|
||||
function crosscheck(bam::BAM.Record, sam::SAM.Record, property::Symbol)
|
||||
|
||||
bam_property = getproperty(XAM.BAM, property)
|
||||
sam_property = getproperty(XAM.SAM, property)
|
||||
|
||||
if bam_property(bam) != sam_property(sam)
|
||||
@warn "$property result is not the same" bam_property(bam) sam_property(sam)
|
||||
return false
|
||||
end
|
||||
|
||||
return true
|
||||
end
|
||||
|
||||
samdir = path_of_format("SAM")
|
||||
bamdir = path_of_format("BAM")
|
||||
|
||||
filenames = [
|
||||
"ce#1",
|
||||
"ce#2",
|
||||
"ce#5",
|
||||
"ce#5b",
|
||||
"ce#unmap",
|
||||
"ce#unmap1",
|
||||
"ce#unmap2",
|
||||
]
|
||||
|
||||
properties = [
|
||||
:position,# POS
|
||||
:tempname,# QNAME
|
||||
:mappingquality,# MAPQ
|
||||
:cigar, # CIGAR
|
||||
:flag, # FLAG
|
||||
:sequence, # SEQ
|
||||
:nextposition, # PNEXT
|
||||
:templength, # TLEN
|
||||
]
|
||||
|
||||
for filename in filenames
|
||||
|
||||
records_bam = collect(open(BAM.Reader, joinpath(bamdir, filename * ".bam")))
|
||||
records_sam = collect(open(SAM.Reader, joinpath(samdir, filename * ".sam")))
|
||||
|
||||
for (bam, sam) in zip(records_bam, records_sam)
|
||||
@test all(crosscheck.(bam, sam, properties)) == true
|
||||
end
|
||||
|
||||
end
|
||||
|
||||
end # testset Crosscheck
|
Loading…
Reference in a new issue