#!/bin/bash #SBATCH --account=cowusda2016 #SBATCH --ntasks=1 #SBATCH --cpus-per-task=4 #SBATCH --mem=8G # Fetch the category we're working with from upstream CATEGORY=${1%} # Save the folder name we will be saving everything into FOLDERNAME="${CATEGORY}-classifier" # Load the required modules module restore system module load swset module load miniconda3 # Start up qiime # This code creates errors if run through shellcheck because # the shellcheck program doesn't understand miniconda: # We'll add a directive to tell it to ignore this error # shellcheck disable=SC1091 source activate qiime2 # Make sure we have a clean slate to work with echo "--^-- X: Creating a classifier model for ${CATEGORY}" echo "--^-- X: Clearing previous classifier results..." rm -r "$FOLDERNAME" echo "--^-- X: Clearing previous classifier results...Done!" # Solve the model echo "--^-- X: Constructing model..." qiime sample-classifier classify-samples \ --i-table feature-table.qza \ --m-metadata-file metadata.tsv \ --m-metadata-column "$CATEGORY" \ --p-n-jobs 4 \ --p-missing-samples ignore \ --p-optimize-feature-selection \ --output-dir "$FOLDERNAME" \ --verbose echo "--^-- X: Constructing model...Done!" # Convert the model output into readable visualizations echo "--^-- X: Making visualizations..." qiime metadata tabulate \ --m-input-file "${FOLDERNAME}/feature_importance.qza" \ --o-visualization "${FOLDERNAME}/feature-importance.qzv" qiime metadata tabulate \ --m-input-file "${FOLDERNAME}/predictions.qza" \ --m-input-file metadata.tsv \ --o-visualization "${FOLDERNAME}/predictions.qzv" echo "--^-- X: Making visualizations...Done!"