diff --git a/docs/uppmax.md b/docs/uppmax.md index a38c1be..176adf3 100644 --- a/docs/uppmax.md +++ b/docs/uppmax.md @@ -8,18 +8,18 @@ We have a Slack channel dedicated to UPPMAX users on the nf-core Slack: [https:/ ## Using the UPPMAX config profile -Before running the pipeline you will need to either install Nextflow or load it using the environment module system (this can be done with e.g. `module load bioinfo-tools Nextflow/` where `VERSION` is e.g. `20.10`). +Before running the pipeline you will need to either install `Nextflow` or load it using the environment module system (this can be done with e.g. `module load bioinfo-tools Nextflow/` where `VERSION` is e.g. `20.10`). To use, run the pipeline with `-profile uppmax` (one hyphen). This will download and launch the [`uppmax.config`](../conf/uppmax.config) which has been pre-configured with a setup suitable for the UPPMAX servers. -It will enable Nextflow to manage the pipeline jobs via the Slurm job scheduler. -Using this profile, Docker image(s) containing required software(s) will be downloaded, and converted to Singularity image(s) if needed before execution of the pipeline. +It will enable `Nextflow` to manage the pipeline jobs via the `Slurm` job scheduler. +Using this profile, `Docker` image(s) containing required software(s) will be downloaded, and converted to `Singularity` image(s) if needed before execution of the pipeline. -Recent version of Nextflow also support the environment variable `NXF_SINGULARITY_CACHEDIR` which can be used to supply images. -Images for some nf-core pipelines are available under `/sw/data/ToolBox/nf-core/` and those can be used by `NXF_SINGULARITY_CACHEDIR=/sw/data/ToolBox/nf-core/; export NXF_SINGULARITY_CACHEDIR`. +Recent version of `Nextflow` also support the environment variable `NXF_SINGULARITY_CACHEDIR` which can be used to supply images. +Images for some `nf-core` pipelines are available under `/sw/data/ToolBox/nf-core/` and those can be used by `NXF_SINGULARITY_CACHEDIR=/sw/data/ToolBox/nf-core/; export NXF_SINGULARITY_CACHEDIR`. In addition to this config profile, you will also need to specify an UPPMAX project id. -You can do this with the `--project` flag (two hyphens) when launching nextflow. +You can do this with the `--project` flag (two hyphens) when launching `Nextflow`. For example: ```bash @@ -29,19 +29,19 @@ $ nextflow run nf-core/ -profile uppmax --project snic2018-1-234 [...] > NB: If you're not sure what your UPPMAX project ID is, try running `groups` or checking SUPR. -Just run Nextflow on a login node and it will handle everything else. +Just run `Nextflow` on a login node and it will handle everything else. -Remember to use `-bg` to launch Nextflow in the background, so that the pipeline doesn't exit if you leave your terminal session. -Alternatively, you can also launch Nextflow in a `screen` or a `tmux` session. +Remember to use `-bg` to launch `Nextflow` in the background, so that the pipeline doesn't exit if you leave your terminal session. +Alternatively, you can also launch `Nextflow` in a `screen` or a `tmux` session. -## Using iGenomes references +## Using AWS iGenomes references -A local copy of the iGenomes resource has been made available on all UPPMAX clusters so you should be able to run the pipeline against any reference available in the `conf/igenomes.config`. +A local copy of the `AWS iGenomes` resource has been made available on all UPPMAX clusters so you should be able to run the pipeline against any reference available in the `conf/igenomes.config`. You can do this by simply using the `--genome ` parameter. ## Getting more memory -If your nf-core pipeline run is running out of memory, you can run on a fat node with more memory using the following nextflow flags: +If your `nf-core` pipeline run is running out of memory, you can run on a fat node with more memory using the following `Nextflow` flags: ```bash --clusterOptions "-C mem256GB" --max_memory "256GB" @@ -96,7 +96,15 @@ If you use `rackham` to download the pipeline and the singularity containers, we ### Download and install Nextflow -You can use the Nextflow UPPMAX provided `module`, but if necessary, you can also download a more recent version. +You can use the `Nextflow` UPPMAX provided `module`, but if necessary, you can also download a more recent version. + +```bash +# See the available versions for the module +module spider Nextflow + +# Load a specific version of the Nextflow module +module load bioinfo-tools Nextflow/` +``` ```bash # Connect to rackham @@ -149,6 +157,19 @@ $ export NXF_SINGULARITY_CACHEDIR=/castor/project/proj_nobackup/singularity-imag ### Install nf-core tools +You can use the `nf-core` UPPMAX provided `module`, but if necessary, you can also download a more recent version. + +```bash +# Connect to rackham +$ ssh -X @rackham.uppmax.uu.se + +# See the available versions for the module +module spider nf-core + +# Load a specific version of the nf-core module +module load bioinfo-tools nf-core/` +``` + ```bash # Connect to rackham $ ssh -X @rackham.uppmax.uu.se