diff --git a/conf/pipeline/eager/shh.config b/conf/pipeline/eager/shh.config index ce5e6c4..ec7bff1 100644 --- a/conf/pipeline/eager/shh.config +++ b/conf/pipeline/eager/shh.config @@ -73,8 +73,8 @@ profiles { bwa_index = '/projects1/Reference_Genomes/Human/HG19/hg19_complete.fasta' fasta_index = '/projects1/Reference_Genomes/Human/HG19/hg19_complete.fasta.fai' seq_dict = '/projects1/Reference_Genomes/Human/HG19/hg19_complete.dict' - bwaalnn = 0.04 - bwaalnl = 32 + bwaalnn = 0.01 + bwaalnl = 16 run_bam_filtering = true bam_discard_unmapped = true bam_unmapped_type = 'fastq' @@ -82,11 +82,11 @@ profiles { metagenomic_tool = 'malt' metagenomic_min_support_reads = 1 database = '/projects1/malt/databases/indexed/index040/full-bac-full-vir-etal-nov_2017' - percent_identity = 90 + percent_identity = 85 malt_mode = 'BlastN' malt_alignment_mode = 'SemiGlobal' malt_top_percent = 1 - malt_min_support_mode = 'percent' + malt_min_support_mode = 'reads' malt_min_support_percent = 0.01 malt_max_queries = 100 malt_memory_mode = 'load' @@ -99,10 +99,9 @@ profiles { maltextract_downsamplingoff = false maltextract_duplicateremovaloff = false maltextract_matches = false - maltextract_megansummary = false - maltextract_percentidentity = 90.0 + maltextract_megansummary = true + maltextract_percentidentity = 85.0 maltextract_topalignment = false - maltextract_singlestranded = false } } pathogen_loose {