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# nf-core/configs: Section for Hologenomics at GLOBE, Univeristy of Copenhagen (Mjolnir server) Configuration
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> **NB:** You will need an account on Mjolnir to run the pipeline. If in doubt contact IT.
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Prior to runing the pipeline for the first time, users must create a hidden directory called .tmp_eager in their user/work directory where the tmp files from nf-core/eager will be re-directed by the NXF_TEMP command (see below).
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```bash
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#navigate into correct directory
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cd /maps/projects/mjolnir1/people/$USER
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#create .tmp_eager directory. This is a hidden directory.
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mkdir .tmp_eager
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```
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The contents of the .tmp_eager directory should be periodically deleted to save on space.
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If the NXF_TEMP command is not used to properly re-direct tmp files the /tmp directory on the compute nodes will be used and quickly filled up, which will make it so that noone can work on these nodes until the files are removed.
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The following lines **must** be added by users to their .bash_profile:
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```bash
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#re-direct tmp files away from /tmp directories on compute nodes or the headnode
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export NXF_TEMP=/maps/projects/mjolnir1/people/$USER/.tmp_eager
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# nextflow - limiting memory of virtual java machine
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NXF_OPTS='-Xms1g -Xmx4g'
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```
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The profile is configured to run with Singularity
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Before running the pipeline you will need to load Java, Miniconda, Singularity and Nextflow. You can do this by including the commands below in your SLURM/sbatch script:
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```bash
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## Load Java and Nextflow environment modules
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module purge
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module load jdk/1.8.0_291 miniconda/4.9.2 singularity/3.8.0 nextflow/21.04.1.5556
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```
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All of the intermediate files required to run the pipeline will be stored in the `work/` directory. It is recommended to delete this directory after the pipeline has finished successfully because it can get quite large, and all of the main output files will be saved in the `results/` directory anyway.
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The config contains a `cleanup` command that removes the `work/` directory automatically once the pipeline has completeed successfully. If the run does not complete successfully then the `work/` dir should be removed manually to save storage space.
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This configuration will automatically choose the correct SLURM queue (short,medium,long) depending on the time and memory required by each process.
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> **NB:** Nextflow will need to submit the jobs via SLURM to the HPC cluster and as such the commands above will have to be submitted from one of the login nodes.
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