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Merge branch 'master' of github.com:nf-core/configs into feat/hasta_update

This commit is contained in:
Ramprasad Neethiraj 2022-08-18 15:58:56 +02:00
commit 7ff2977a29
37 changed files with 845 additions and 64 deletions

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@ -8,5 +8,5 @@ trim_trailing_whitespace = true
indent_size = 4 indent_size = 4
indent_style = space indent_style = space
[*.{md,yml,yaml}] [*.{md,yml,yaml,cff}]
indent_size = 2 indent_size = 2

55
.github/workflows/fix-linting.yml vendored Normal file
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@ -0,0 +1,55 @@
name: Fix linting from a comment
on:
issue_comment:
types: [created]
jobs:
deploy:
# Only run if comment is on a PR with the main repo, and if it contains the magic keywords
if: >
contains(github.event.comment.html_url, '/pull/') &&
contains(github.event.comment.body, '@nf-core-bot fix linting') &&
github.repository == 'nf-core/configs'
runs-on: ubuntu-latest
steps:
# Use the @nf-core-bot token to check out so we can push later
- uses: actions/checkout@v3
with:
token: ${{ secrets.nf_core_bot_auth_token }}
# Action runs on the issue comment, so we don't get the PR by default
# Use the gh cli to check out the PR
- name: Checkout Pull Request
run: gh pr checkout ${{ github.event.issue.number }}
env:
GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}
- uses: actions/setup-node@v2
- name: Install Prettier
run: npm install -g prettier @prettier/plugin-php
# Check that we actually need to fix something
- name: Run 'prettier --check'
id: prettier_status
run: |
if prettier --check ${GITHUB_WORKSPACE}; then
echo "::set-output name=result::pass"
else
echo "::set-output name=result::fail"
fi
- name: Run 'prettier --write'
if: steps.prettier_status.outputs.result == 'fail'
run: prettier --write ${GITHUB_WORKSPACE}
- name: Commit & push changes
if: steps.prettier_status.outputs.result == 'fail'
run: |
git config user.email "core@nf-co.re"
git config user.name "nf-core-bot"
git config push.default upstream
git add .
git status
git commit -m "[automated] Fix linting with Prettier"
git push

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@ -43,6 +43,7 @@ jobs:
- "cbe" - "cbe"
- "ccga_dx" - "ccga_dx"
- "ccga_med" - "ccga_med"
- "cedars"
- "cfc" - "cfc"
- "cfc_dev" - "cfc_dev"
- "cheaha" - "cheaha"
@ -65,6 +66,8 @@ jobs:
- "jax" - "jax"
- "lugh" - "lugh"
- "marvin" - "marvin"
- "medair"
- "mjolnir_globe"
- "maestro" - "maestro"
- "mpcdf" - "mpcdf"
- "munin" - "munin"
@ -75,6 +78,7 @@ jobs:
- "phoenix" - "phoenix"
- "prince" - "prince"
- "rosalind" - "rosalind"
- "sage"
- "sahmri" - "sahmri"
- "sanger" - "sanger"
- "seg_globe" - "seg_globe"
@ -85,6 +89,7 @@ jobs:
- "utd_sysbio" - "utd_sysbio"
- "uzh" - "uzh"
- "vai" - "vai"
- "vsc_ugent"
steps: steps:
- uses: actions/checkout@v1 - uses: actions/checkout@v1
- name: Install Nextflow - name: Install Nextflow

56
CITATION.cff Normal file
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@ -0,0 +1,56 @@
cff-version: 1.2.0
message: "If you use `nf-core tools` in your work, please cite the `nf-core` publication"
authors:
- family-names: Ewels
given-names: Philip
- family-names: Peltzer
given-names: Alexander
- family-names: Fillinger
given-names: Sven
- family-names: Patel
given-names: Harshil
- family-names: Alneberg
given-names: Johannes
- family-names: Wilm
given-names: Andreas
- family-names: Ulysse Garcia
given-names: Maxime
- family-names: Di Tommaso
given-names: Paolo
- family-names: Nahnsen
given-names: Sven
title: "The nf-core framework for community-curated bioinformatics pipelines."
version: 2.4.1
doi: 10.1038/s41587-020-0439-x
date-released: 2022-05-16
url: https://github.com/nf-core/tools
prefered-citation:
type: article
authors:
- family-names: Ewels
given-names: Philip
- family-names: Peltzer
given-names: Alexander
- family-names: Fillinger
given-names: Sven
- family-names: Patel
given-names: Harshil
- family-names: Alneberg
given-names: Johannes
- family-names: Wilm
given-names: Andreas
- family-names: Ulysse Garcia
given-names: Maxime
- family-names: Di Tommaso
given-names: Paolo
- family-names: Nahnsen
given-names: Sven
doi: 10.1038/s41587-020-0439-x
journal: nature biotechnology
start: 276
end: 278
title: "The nf-core framework for community-curated bioinformatics pipelines."
issue: 3
volume: 38
year: 2020
url: https://dx.doi.org/10.1038/s41587-020-0439-x

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@ -10,7 +10,6 @@ A repository for hosting Nextflow configuration files containing custom paramete
- [Configuration and parameters](#configuration-and-parameters) - [Configuration and parameters](#configuration-and-parameters)
- [Offline usage](#offline-usage) - [Offline usage](#offline-usage)
- [Adding a new config](#adding-a-new-config) - [Adding a new config](#adding-a-new-config)
- [Checking user hostnames](#checking-user-hostnames)
- [Testing](#testing) - [Testing](#testing)
- [Documentation](#documentation) - [Documentation](#documentation)
- [Uploading to `nf-core/configs`](#uploading-to-nf-coreconfigs) - [Uploading to `nf-core/configs`](#uploading-to-nf-coreconfigs)
@ -99,6 +98,7 @@ Currently documentation is available for the following systems:
- [CBE](docs/cbe.md) - [CBE](docs/cbe.md)
- [CCGA_DX](docs/ccga_dx.md) - [CCGA_DX](docs/ccga_dx.md)
- [CCGA_MED](docs/ccga_med.md) - [CCGA_MED](docs/ccga_med.md)
- [Cedars-Sinai](docs/cedars.md)
- [CFC](docs/cfc.md) - [CFC](docs/cfc.md)
- [CHEAHA](docs/cheaha.md) - [CHEAHA](docs/cheaha.md)
- [Computerome](docs/computerome.md) - [Computerome](docs/computerome.md)
@ -120,6 +120,8 @@ Currently documentation is available for the following systems:
- [LUGH](docs/lugh.md) - [LUGH](docs/lugh.md)
- [MAESTRO](docs/maestro.md) - [MAESTRO](docs/maestro.md)
- [MARVIN](docs/marvin.md) - [MARVIN](docs/marvin.md)
- [MEDAIR](docs/medair.md)
- [MJOLNIR_GLOBE](docs/mjolnir_globe.md)
- [MPCDF](docs/mpcdf.md) - [MPCDF](docs/mpcdf.md)
- [MUNIN](docs/munin.md) - [MUNIN](docs/munin.md)
- [NU_GENOMICS](docs/nu_genomics.md) - [NU_GENOMICS](docs/nu_genomics.md)
@ -129,6 +131,7 @@ Currently documentation is available for the following systems:
- [PHOENIX](docs/phoenix.md) - [PHOENIX](docs/phoenix.md)
- [PRINCE](docs/prince.md) - [PRINCE](docs/prince.md)
- [ROSALIND](docs/rosalind.md) - [ROSALIND](docs/rosalind.md)
- [SAGE BIONETWORKS](docs/sage.md)
- [SANGER](docs/sanger.md) - [SANGER](docs/sanger.md)
- [SEG_GLOBE](docs/seg_globe.md) - [SEG_GLOBE](docs/seg_globe.md)
- [UCT_HPC](docs/uct_hpc.md) - [UCT_HPC](docs/uct_hpc.md)
@ -138,6 +141,7 @@ Currently documentation is available for the following systems:
- [UTD_SYSBIO](docs/utd_sysbio.md) - [UTD_SYSBIO](docs/utd_sysbio.md)
- [UZH](docs/uzh.md) - [UZH](docs/uzh.md)
- [VAI](docs/vai.md) - [VAI](docs/vai.md)
- [VSC_UGENT](docs/vsc_ugent.md)
### Uploading to `nf-core/configs` ### Uploading to `nf-core/configs`
@ -191,13 +195,18 @@ Currently documentation is available for the following pipelines within specific
- [UPPMAX](docs/pipeline/ampliseq/uppmax.md) - [UPPMAX](docs/pipeline/ampliseq/uppmax.md)
- eager - eager
- [EVA](docs/pipeline/eager/eva.md) - [EVA](docs/pipeline/eager/eva.md)
- mag
- [EVA](docs/pipeline/mag/eva.md)
- rnafusion - rnafusion
- [MUNIN](docs/pipeline/rnafusion/munin.md) - [MUNIN](docs/pipeline/rnafusion/munin.md)
- rnavar
- [MUNIN](docs/pipeline/rnavar/munin.md)
- sarek - sarek
- [MUNIN](docs/pipeline/sarek/munin.md) - [MUNIN](docs/pipeline/sarek/munin.md)
- [UPPMAX](docs/pipeline/sarek/uppmax.md) - [UPPMAX](docs/pipeline/sarek/uppmax.md)
- rnavar - taxprofiler
- [MUNIN](docs/pipeline/rnavar/munin.md) - [EVA](docs/pipeline/taxprofiler/eva.md)
- [hasta](docs/pipeline/taxprofiler/hasta.md)
### Pipeline-specific documentation ### Pipeline-specific documentation

26
conf/cedars.config Normal file
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@ -0,0 +1,26 @@
//Profile config names for nf-core/configs
params {
config_profile_description = 'Cedars-Sinai Medical Center HPC Profile'
config_profile_contact = 'Alex Rajewski (@rajewski)'
config_profile_url = 'https://www.cedars-sinai.edu/research/cores/informatics-computing/resources.html'
max_memory = 90.GB
max_cpus = 10
max_time = 240.h
}
// Specify the queing system
executor {
name = "sge"
}
process {
penv = 'smp'
beforeScript =
"""
module load 'singularity/3.6.0'
"""
}
singularity {
enabled = true
}

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@ -17,7 +17,7 @@ process {
executor = 'sge' executor = 'sge'
penv = 'smp' penv = 'smp'
queue = 'all.q' queue = 'all.q'
clusterOptions = { "-S /bin/bash -V -j y -o output.log -l h_vmem=${task.memory.toGiga()}G" } clusterOptions = { "-S /bin/bash -V -j y -o output.sge -l h_vmem=${task.memory.toGiga()}G" }
} }
executor { executor {
@ -37,7 +37,7 @@ profiles {
process { process {
queue = { task.memory > 700.GB ? 'bigmem.q' : 'archgen.q' } queue = { task.memory > 700.GB ? 'bigmem.q' : 'archgen.q' }
clusterOptions = { "-S /bin/bash -V -j y -o output.log -l h_vmem=${task.memory.toGiga()}G" } clusterOptions = { "-S /bin/bash -V -j y -o output.sge -l h_vmem=${task.memory.toGiga()}G" }
} }
singularity { singularity {

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@ -15,7 +15,8 @@ google.zone = params.google_zone
google.lifeSciences.debug = params.google_debug google.lifeSciences.debug = params.google_debug
workDir = params.google_bucket workDir = params.google_bucket
google.lifeSciences.preemptible = params.google_preemptible google.lifeSciences.preemptible = params.google_preemptible
if (google.lifeSciences.preemptible) { if (google.lifeSciences.preemptible) {
process.errorStrategy = { task.exitStatus==14 ? 'retry' : 'terminate' } process.errorStrategy = { task.exitStatus in [8,10,14] ? 'retry' : 'terminate' }
process.maxRetries = 5 process.maxRetries = 5
} }

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@ -10,6 +10,7 @@ params {
singularity { singularity {
enabled = true enabled = true
envWhitelist = ['_JAVA_OPTIONS']
} }
params { params {

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@ -2,23 +2,22 @@
params { params {
config_profile_description = 'The IFB core cluster profile' config_profile_description = 'The IFB core cluster profile'
config_profile_contact = 'https://community.france-bioinformatique.fr' config_profile_contact = 'https://community.france-bioinformatique.fr'
config_profile_url = 'https://www.france-bioinformatique.fr/' config_profile_url = 'https://ifb-elixirfr.gitlab.io/cluster/doc/cluster-desc/'
} }
singularity { singularity {
// need one image per execution
enabled = true enabled = true
runOptions = '-B /shared' runOptions = '-B /shared'
} }
process { process {
executor = 'slurm' executor = 'slurm'
queue = { task.time <= 24.h ? 'fast' : 'long' }
} }
params { params {
igenomes_ignore = true igenomes_ignore = true
// Max resources requested by a normal node on genotoul. max_memory = 252.GB
max_memory = 240.GB max_cpus = 56
max_cpus = 28 max_time = 720.h
max_time = 96.h
} }

46
conf/medair.config Normal file
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@ -0,0 +1,46 @@
//Profile config names for nf-core/configs
params {
config_profile_description = 'Cluster profile for medair (local cluster of Clinical Genomics Gothenburg)'
config_profile_contact = 'Clinical Genomics, Gothenburg (cgg-rd@gu.se, cgg-it@gu.se)'
config_profile_url = 'https://www.scilifelab.se/units/clinical-genomics-goteborg/'
}
//Nextflow parameters
singularity {
enabled = true
cacheDir = "/apps/bio/dependencies/nf-core/singularities"
}
profiles {
wgs {
process {
queue = 'wgs.q'
executor = 'sge'
penv = 'mpi'
process.clusterOptions = '-l excl=1'
params.max_cpus = 40
params.max_time = 48.h
params.max_memory = 128.GB
}
}
production {
process {
queue = 'production.q'
executor = 'sge'
penv = 'mpi'
process.clusterOptions = '-l excl=1'
params.max_cpus = 40
params.max_time = 480.h
params.max_memory = 128.GB
}
}
}
//Specific parameter for pipelines that can use Sentieon (e.g. nf-core/sarek, nf-core/raredisease)
process {
withLabel:'sentieon' {
container = "/apps/bio/singularities/sentieon-211204-peta.simg"
}
}

25
conf/mjolnir_globe.config Normal file
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@ -0,0 +1,25 @@
//Profile config names for nf-core/configs
params {
config_profile_description = 'Section for Hologenomics and Section for Molecular Ecology and Evolution @ Globe Institute, University of Copenhagen - mjolnir_globe profile provided by nf-core/configs.'
config_profile_contact = 'Aashild Vaagene (@ashildv)'
config_profile_url = 'https://globe.ku.dk/research/'
max_memory = 500.GB
max_cpus = 50
max_time = 720.h
}
singularity {
enabled = true
autoMounts = true
cacheDir = '/maps/projects/mjolnir1/data/cache/nf-core/singularity'
}
process {
executor = 'slurm'
}
cleanup = true
executor {
queueSize = 10
}

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@ -61,7 +61,7 @@ profiles {
params { params {
config_profile_description = 'MPCDF raven profile (unofficially) provided by nf-core/configs.' config_profile_description = 'MPCDF raven profile (unofficially) provided by nf-core/configs.'
memory = 2000000.MB max_memory = 2000000.MB
max_cpus = 72 max_cpus = 72
max_time = 24.h max_time = 24.h
} }

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@ -6,11 +6,15 @@ params {
config_profile_description = 'nf-core/eager EVA profile provided by nf-core/configs' config_profile_description = 'nf-core/eager EVA profile provided by nf-core/configs'
} }
env {
_JAVA_OPTIONS = "-XX:ParallelGCThreads=1"
OPENBLAS_NUM_THREADS = 1
OMP_NUM_THREADS = 1
}
// Specific nf-core/eager process configuration // Specific nf-core/eager process configuration
process { process {
beforeScript = 'export _JAVA_OPTIONS="-XX:ParallelGCThreads=1"'
maxRetries = 2 maxRetries = 2
// Solution for clusterOptions comes from here: https://github.com/nextflow-io/nextflow/issues/332 + personal toMega conversion // Solution for clusterOptions comes from here: https://github.com/nextflow-io/nextflow/issues/332 + personal toMega conversion
@ -70,11 +74,16 @@ process {
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' } errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
} }
withName: fastqc_after_clipping {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 2)}G" }
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
}
withName: adapter_removal { withName: adapter_removal {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 2)}G" } clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 2)}G" }
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' } errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
} }
withName: bwa { withName: bwa {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga())}G,h=!(bionode01|bionode02|bionode03|bionode04|bionode05|bionode06)" } clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga())}G,h=!(bionode01|bionode02|bionode03|bionode04|bionode05|bionode06)" }
} }
@ -184,6 +193,17 @@ process {
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' } errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
} }
withName:get_software_versions {
cache = false
clusterOptions = { "-S /bin/bash -V -l h=!(bionode06)" }
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toMega() * 8)}M" }
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
}
withName:multiqc {
clusterOptions = { "-S /bin/bash -V -j y -o output.log -l h_vmem=${task.memory.toGiga() * 2}G" }
}
} }
profiles { profiles {
@ -202,8 +222,6 @@ profiles {
process { process {
beforeScript = 'export _JAVA_OPTIONS="-XX:ParallelGCThreads=1"'
maxRetries = 2 maxRetries = 2
// Solution for clusterOptions comes from here: https://github.com/nextflow-io/nextflow/issues/332 + personal toMega conversion // Solution for clusterOptions comes from here: https://github.com/nextflow-io/nextflow/issues/332 + personal toMega conversion
@ -256,6 +274,11 @@ profiles {
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' } errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
} }
withName: fastqc_after_clipping {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 3)}G" }
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
}
withName: adapter_removal { withName: adapter_removal {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 3)}G" } clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 3)}G" }
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' } errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
@ -358,7 +381,6 @@ profiles {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 3)}G" } clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 3)}G" }
errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'ignore' } errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'ignore' }
} }
} }
} }
@ -376,8 +398,6 @@ profiles {
process { process {
beforeScript = 'export _JAVA_OPTIONS="-XX:ParallelGCThreads=1"'
maxRetries = 2 maxRetries = 2
// Solution for clusterOptions comes from here: https://github.com/nextflow-io/nextflow/issues/332 + personal toMega conversion // Solution for clusterOptions comes from here: https://github.com/nextflow-io/nextflow/issues/332 + personal toMega conversion
@ -430,6 +450,11 @@ profiles {
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' } errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
} }
withName: fastqc_after_clipping {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 6)}G" }
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
}
withName: adapter_removal { withName: adapter_removal {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 6)}G" } clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 6)}G" }
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' } errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
@ -533,7 +558,6 @@ profiles {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 6)}G" } clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 6)}G" }
errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'ignore' } errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'ignore' }
} }
} }
} }

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@ -0,0 +1,14 @@
params {
// Specific nf-core/configs params
config_profile_contact = 'James Fellows Yates (@jfy133)'
config_profile_description = 'nf-core/mag EVA profile provided by nf-core/configs'
}
process {
withName: FASTQC {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 2)}G" }
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
}
}

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@ -20,6 +20,7 @@ process {
enabled: false, enabled: false,
] ]
} }
withName:'QUALIMAP_BAMQC' {
ext.args = { "--java-mem-size=${task.memory.giga / 1.15 as long}G" }
}
} }

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@ -0,0 +1,29 @@
params {
// Specific nf-core/configs params
config_profile_contact = 'James Fellows Yates (@jfy133)'
config_profile_description = 'nf-core/taxprofiler EVA profile provided by nf-core/configs'
}
process {
withName: BBMAP_BBDUK {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 2)}G" }
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
}
withName: MALT_RUN {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 4)}G" }
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
}
withName: METAPHLAN3 {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 3)}G" }
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
}
withName: MEGAN_RMA2INFO {
clusterOptions = { "-S /bin/bash -V -l h_vmem=${(task.memory.toGiga() * 4)}G" }
errorStrategy = { task.exitStatus in [1,143,137,104,134,139,140] ? 'retry' : 'finish' }
}
}

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@ -0,0 +1,16 @@
params {
// Specific nf-core/configs params
config_profile_contact = 'Sofia Stamouli (@sofstam)'
config_profile_description = 'nf-core/taxprofiler HASTA profile provided by nf-core/configs'
}
process {
withName:'BBMAP_BBDUK' {
memory = { check_max( 80.GB * task.attempt, 'memory' ) }
}
withName: 'MALT_RUN' {
memory = { check_max( 80.GB * task.attempt, 'memory' ) }
}
}

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@ -13,18 +13,18 @@ params {
// Please use 'MN908947.3' if possible because all primer sets are available / have been pre-prepared relative to that assembly // Please use 'MN908947.3' if possible because all primer sets are available / have been pre-prepared relative to that assembly
fasta = 'https://github.com/nf-core/test-datasets/raw/viralrecon/genome/NC_045512.2/GCF_009858895.2_ASM985889v3_genomic.200409.fna.gz' fasta = 'https://github.com/nf-core/test-datasets/raw/viralrecon/genome/NC_045512.2/GCF_009858895.2_ASM985889v3_genomic.200409.fna.gz'
gff = 'https://github.com/nf-core/test-datasets/raw/viralrecon/genome/NC_045512.2/GCF_009858895.2_ASM985889v3_genomic.200409.gff.gz' gff = 'https://github.com/nf-core/test-datasets/raw/viralrecon/genome/NC_045512.2/GCF_009858895.2_ASM985889v3_genomic.200409.gff.gz'
nextclade_dataset = 'https://github.com/nf-core/test-datasets/raw/viralrecon/genome/MN908947.3/nextclade_sars-cov-2_MN908947_2022-01-18T12_00_00Z.tar.gz' nextclade_dataset = 'https://github.com/nf-core/test-datasets/raw/viralrecon/genome/MN908947.3/nextclade_sars-cov-2_MN908947_2022-06-14T12_00_00Z.tar.gz'
nextclade_dataset_name = 'sars-cov-2' nextclade_dataset_name = 'sars-cov-2'
nextclade_dataset_reference = 'MN908947' nextclade_dataset_reference = 'MN908947'
nextclade_dataset_tag = '2022-01-18T12:00:00Z' nextclade_dataset_tag = '2022-06-14T12:00:00Z'
} }
'MN908947.3' { 'MN908947.3' {
fasta = 'https://github.com/nf-core/test-datasets/raw/viralrecon/genome/MN908947.3/GCA_009858895.3_ASM985889v3_genomic.200409.fna.gz' fasta = 'https://github.com/nf-core/test-datasets/raw/viralrecon/genome/MN908947.3/GCA_009858895.3_ASM985889v3_genomic.200409.fna.gz'
gff = 'https://github.com/nf-core/test-datasets/raw/viralrecon/genome/MN908947.3/GCA_009858895.3_ASM985889v3_genomic.200409.gff.gz' gff = 'https://github.com/nf-core/test-datasets/raw/viralrecon/genome/MN908947.3/GCA_009858895.3_ASM985889v3_genomic.200409.gff.gz'
nextclade_dataset = 'https://github.com/nf-core/test-datasets/raw/viralrecon/genome/MN908947.3/nextclade_sars-cov-2_MN908947_2022-01-18T12_00_00Z.tar.gz' nextclade_dataset = 'https://github.com/nf-core/test-datasets/raw/viralrecon/genome/MN908947.3/nextclade_sars-cov-2_MN908947_2022-06-14T12_00_00Z.tar.gz'
nextclade_dataset_name = 'sars-cov-2' nextclade_dataset_name = 'sars-cov-2'
nextclade_dataset_reference = 'MN908947' nextclade_dataset_reference = 'MN908947'
nextclade_dataset_tag = '2022-01-18T12:00:00Z' nextclade_dataset_tag = '2022-06-14T12:00:00Z'
primer_sets { primer_sets {
artic { artic {
'1' { '1' {

100
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@ -0,0 +1,100 @@
params {
config_profile_description = 'The Sage Bionetworks profile'
config_profile_contact = 'Bruno Grande (@BrunoGrandePhD)'
config_profile_url = 'https://github.com/Sage-Bionetworks-Workflows'
}
process {
cpus = { check_max( 1 * slow(task.attempt), 'cpus' ) }
memory = { check_max( 6.GB * task.attempt, 'memory' ) }
time = { check_max( 24.h * task.attempt, 'time' ) }
errorStrategy = { task.exitStatus in [143,137,104,134,139,247] ? 'retry' : 'finish' }
maxRetries = 5
maxErrors = '-1'
// Process-specific resource requirements
withLabel:process_low {
cpus = { check_max( 4 * slow(task.attempt), 'cpus' ) }
memory = { check_max( 12.GB * task.attempt, 'memory' ) }
time = { check_max( 24.h * task.attempt, 'time' ) }
}
withLabel:process_medium {
cpus = { check_max( 12 * slow(task.attempt), 'cpus' ) }
memory = { check_max( 36.GB * task.attempt, 'memory' ) }
time = { check_max( 48.h * task.attempt, 'time' ) }
}
withLabel:process_high {
cpus = { check_max( 24 * slow(task.attempt), 'cpus' ) }
memory = { check_max( 72.GB * task.attempt, 'memory' ) }
time = { check_max( 96.h * task.attempt, 'time' ) }
}
withLabel:process_long {
time = { check_max( 192.h * task.attempt, 'time' ) }
}
withLabel:process_high_memory {
memory = { check_max( 128.GB * task.attempt, 'memory' ) }
}
// Preventing Sarek labels from using the actual maximums
withLabel:memory_max {
memory = { check_max( 128.GB * task.attempt, 'memory' ) }
}
withLabel:cpus_max {
cpus = { check_max( 24 * slow(task.attempt), 'cpus' ) }
}
}
aws {
region = "us-east-1"
}
params {
igenomes_base = 's3://sage-igenomes/igenomes'
max_memory = 500.GB
max_cpus = 64
max_time = 168.h // One week
}
// Function to slow the increase of the resource multipler
// as attempts are made. The rationale is that some CPUs
// don't need to be increased as fast as memory.
def slow(attempt, factor = 2) {
return Math.ceil( attempt / factor) as int
}
// Function to ensure that resource requirements don't go
// beyond a maximum limit (copied here for Sarek v2)
def check_max(obj, type) {
if (type == 'memory') {
try {
if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1)
return params.max_memory as nextflow.util.MemoryUnit
else
return obj
} catch (all) {
println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj"
return obj
}
} else if (type == 'time') {
try {
if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1)
return params.max_time as nextflow.util.Duration
else
return obj
} catch (all) {
println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj"
return obj
}
} else if (type == 'cpus') {
try {
return Math.min( obj, params.max_cpus as int )
} catch (all) {
println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj"
return obj
}
}
}

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@ -1,35 +1,33 @@
// Profile details
params { params {
config_profile_description = 'The Wellcome Sanger Institute HPC cluster profile' config_profile_description = 'The Wellcome Sanger Institute HPC cluster (farm5) profile'
config_profile_contact = 'Anthony Underwood (@aunderwo)' config_profile_contact = 'Priyanka Surana (@priyanka-surana)'
config_profile_url = 'https://www.sanger.ac.uk/group/informatics-support-group/' config_profile_url = 'https://www.sanger.ac.uk'
}
singularity {
enabled = true
cacheDir = "${baseDir}/singularity"
runOptions = '--bind /lustre --bind /nfs/pathnfs01 --bind /nfs/pathnfs02 --bind /nfs/pathnfs03 --bind /nfs/pathnfs04 --bind /nfs/pathnfs05 --bind /nfs/pathnfs06 --no-home'
} }
// Queue and retry strategy
process{ process{
executor = 'lsf' executor = 'lsf'
queue = 'normal' queue = { task.time < 12.h ? 'normal' : task.time < 48.h ? 'long' : 'basement' }
errorStrategy = { task.attempt <= 5 ? "retry" : "finish" } errorStrategy = 'retry'
process.maxRetries = 5 maxRetries = 5
withLabel:process_long {
queue = 'long'
}
} }
// Executor details
executor{ executor{
name = 'lsf' name = 'lsf'
perJobMemLimit = true perJobMemLimit = true
poolSize = 4 poolSize = 4
submitRateLimit = '5 sec' submitRateLimit = '5 sec'
killBatchSize = 50 killBatchSize = 50
} }
// Max resources
params { params {
max_memory = 128.GB max_memory = 683.GB
max_cpus = 64 max_cpus = 256
max_time = 48.h max_time = 720.h
} }
// For singularity
singularity.runOptions = '--bind /lustre --bind /nfs'

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// Define the Scratch directory
def scratch_dir = System.getenv("VSC_SCRATCH_VO_USER") ?: "scratch/"
// Specify the work directory
workDir = "$scratch_dir/work"
// Perform work directory cleanup when the run has succesfully completed
// cleanup = true
// Reduce the job submit rate to about 5 per second, this way the server won't be bombarded with jobs
executor {
submitRateLimit = '3 sec'
}
// Specify that singularity should be used and where the cache dir will be for the images
singularity {
enabled = true
autoMounts = true
cacheDir = "$scratch_dir/singularity"
}
env {
SINGULARITY_CACHEDIR="$scratch_dir/.singularity"
}
// Define profiles for each cluster
profiles {
skitty {
params {
config_profile_description = 'HPC_SKITTY profile for use on the Skitty cluster of the VSC HPC.'
config_profile_contact = 'Nicolas Vannieuwkerke (@nvnieuwk)'
config_profile_url = 'https://www.ugent.be/hpc/en'
max_memory = 177.GB
max_cpus = 36
max_time = 72.h
}
process {
executor = 'slurm'
queue = 'skitty'
maxRetries = 2
scratch = "$scratch_dir"
}
}
swalot {
params {
config_profile_description = 'HPC_SWALOT profile for use on the Swalot cluster of the VSC HPC.'
config_profile_contact = 'Nicolas Vannieuwkerke (@nvnieuwk)'
config_profile_url = 'https://www.ugent.be/hpc/en'
max_memory = 116.GB
max_cpus = 20
max_time = 72.h
}
process {
executor = 'slurm'
queue = 'swalot'
maxRetries = 2
scratch = "$scratch_dir"
}
}
victini {
params {
config_profile_description = 'HPC_VICTINI profile for use on the Victini cluster of the VSC HPC.'
config_profile_contact = 'Nicolas Vannieuwkerke (@nvnieuwk)'
config_profile_url = 'https://www.ugent.be/hpc/en'
max_memory = 88.GB
max_cpus = 36
max_time = 72.h
}
process {
executor = 'slurm'
queue = 'victini'
maxRetries = 2
scratch = "$scratch_dir"
}
}
kirlia {
params {
config_profile_description = 'HPC_KIRLIA profile for use on the Kirlia cluster of the VSC HPC.'
config_profile_contact = 'Nicolas Vannieuwkerke (@nvnieuwk)'
config_profile_url = 'https://www.ugent.be/hpc/en'
max_memory = 738.GB
max_cpus = 36
max_time = 72.h
}
process {
executor = 'slurm'
queue = 'kirlia'
maxRetries = 2
scratch = "$scratch_dir"
}
}
doduo {
params {
config_profile_description = 'HPC_DODUO profile for use on the Doduo cluster of the VSC HPC.'
config_profile_contact = 'Nicolas Vannieuwkerke (@nvnieuwk)'
config_profile_url = 'https://www.ugent.be/hpc/en'
max_memory = 250.GB
max_cpus = 96
max_time = 72.h
}
process {
executor = 'slurm'
queue = 'doduo'
maxRetries = 2
scratch = "$scratch_dir"
}
}
}

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@ -0,0 +1,7 @@
# Cedars-Sinai Medical Center HPC
- You will need HPC access from EIS, which can be requested in the Service Center.
- You will need to load the nextflow module on the HPC before running any pipelines (`module load nextflow`). This should automatically load Java as well.
- Run this with `-profile cedars`
- By default this config file does not specify a queue for submission, and things will thus go to `all.q`. Because of that, the memory and cpu limits have been set accordingly.
- We highly recommend specifying a location of a cache directory to store singularity images (so you re-use them across runs, and not pull each time), by specifying the location with the `$NXF_SINGULARITY_CACHE_DIR` bash environment variable in your `.bash_profile` or `.bashrc`

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@ -6,7 +6,8 @@ To use, run the pipeline with `-profile ifb_core`. This will download and launch
## How to use on IFB core ## How to use on IFB core
Before running the pipeline you will need to load Nextflow using the environment module system on IFB core. You can do this by issuing the commands below: Here is [the link to the cluster's documentation](https://ifb-elixirfr.gitlab.io/cluster/doc/quick-start/).
Before running the pipeline you will need to load Nextflow and other dependencies using the environment module system on IFB core. You can do this by issuing the commands below:
```bash ```bash
# Login to a compute node # Login to a compute node
@ -14,7 +15,10 @@ srun --pty bash
## Load Nextflow and Singularity environment modules ## Load Nextflow and Singularity environment modules
module purge module purge
module load nextflow/20.04.1 module load nextflow
module load singularity
module load openjdk
# Run a downloaded/git-cloned nextflow workflow from # Run a downloaded/git-cloned nextflow workflow from
nextflow run \\ nextflow run \\

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# nf-core/configs: Medair Configuration
All nf-core pipelines have been successfully configured for use on the Medair cluster at Clinical Genomics Gothenburg.
To use, run the pipeline with `-profile medair`. This will download and launch the [`medair.config`](../conf/medair.config) which has been pre-configured with a setup suitable for the Medair cluster.
It will enable Nextflow to manage the pipeline jobs via the `SGE` job scheduler.
Using this profile, a docker image containing all of the required software will be downloaded, and converted to a Singularity image before execution of the pipeline.
You will need an account to use the Medair cluster in order to download or run pipelines. If in doubt, contact cgg-it.
## Download nf-core pipelines
### Set-up: load Nextflow and nf-core tools
First you need to load relevant softwares: Nextflow and nf-core tools. You can do it as follow:
```bash
## Load Nextflow
module load nextflow
## Load nf-core tools
module load miniconda
source activate nf-core
```
### Storage of Singularity images
When downloading a nf-core pipeline for the first time (or a specific version of a pipeline), you can choose to store the Singularity image for future use. We chose to have a central location for these images on medair: `/apps/bio/dependencies/nf-core/singularities`.
For Nexflow to know where to store new images, run or add the following to your `.bashrc`:
```bash
export NXF_SINGULARITY_CACHEDIR="/apps/bio/dependencies/nf-core/singularities"
```
> Comment: This was also added to cronuser.
### Download a pipeline
We have started to download pipelines in the following location: `/apps/bio/repos/nf-core/`
Use the `nf-core download --singularity-cache-only` command to start a download. It will open an interactive menu. Choose `singularity` for the software container image, and `none` for the compression type.
## Run nf-core pipelines
Nextflow will need to submit the jobs via the job scheduler to the HPC cluster and as such the commands below will have to be executed on one of the login nodes. If in doubt contact cgg-it (cgg-it[at]gu.se).
### Set-up: load Nextflow and Singularity
Before running a pipeline you will need to load Nextflow and Singularity using the environment module system on Medair. You can do this by issuing the commands below:
```bash
## Load Nextflow and Singularity environment modules
module purge
module load nextflow
module load singularity
```
### Choose a profile
Depending on what you are running, you can choose between the `wgs` and `production` profiles. Jobs running with the `wgs` profile run on a queue with higher priority. Jobs running with the `production` profile can last longer (max time: 20 days, versus 2 days for the `wgs` profile).
For example, the following job would run with the `wgs` profile:
```bash
run nextflow nf-core/raredisease -profile medair,wgs
```
### Sentieon
In some pipelines (sarek, raredisease) it is possible to use Sentieon for alignment and variant calling. If ones uses the label `sentieon` for running a process, the config file contains the path to the Sentieon singularity image on Medair.

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# nf-core/configs: Section for Hologenomics at GLOBE, University of Copenhagen (Mjolnir server) Configuration
> **NB:** You will need an account on Mjolnir to run the pipeline. If in doubt contact IT.
Prior to running the pipeline for the first time with the `mjolnir_globe.config` (../conf/mjolnir_globe.config), users **must** create a hidden directory called `.tmp_nfcore` in their data/project directory on Mjolnir where the temp files from nf-core pipelines will be re-directed by the `NXF_TEMP` command (see below).
The contents of the `.tmp_nfcore` directory should be periodically deleted manually to save on space.
If the `NXF_TEMP` command is not used to properly re-direct temp files the `/tmp` directory on the compute nodes will be used and quickly filled up, which blocks anyone from working on these nodes until the offending user removes their files.
The following lines **must** be added by users to their `~/.bash_profile`:
```bash
#re-direct tmp files away from /tmp directories on compute nodes or the headnode
export NXF_TEMP=/maps/projects/mjolnir1/people/$USER/.tmp_nfcore
# nextflow - limiting memory of virtual java machine
NXF_OPTS='-Xms1g -Xmx4g'
```
Once you have created the `.tmp_nfcore` directory and added the above lines of code to your `.bash_profile` you can run an nf-core pipeline.
Before running a pipeline you will need to load Java, Miniconda, Singularity and Nextflow. You can do this by including the commands below in your SLURM/sbatch script:
```bash
## Load Java and Nextflow environment modules
module purge
module load jdk/1.8.0_291 miniconda singularity/3.8.0 nextflow/21.04.1.5556
```
All of the intermediate output files required to run the pipeline will be stored in the `work/` directory. It is recommended to delete this directory after the pipeline has finished successfully because it can get quite large, and all of the main output files will be saved in the `results/` directory anyway.
The `mjolnir_globe` config contains a `cleanup` command that removes the `work/` directory automatically once the pipeline has completeed successfully. If the run does not complete successfully then the `work/` dir should be removed manually to save storage space.

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@ -0,0 +1,15 @@
# nf-core/configs: eva mag specific configuration
Extra specific configuration for mag pipeline
## Usage
To use, run the pipeline with `-profile eva`.
This will download and launch the mag specific [`eva.config`](../../../conf/pipeline/mag/eva.config) which has been pre-configured with a setup suitable for the MPI-EVA cluster.
Example: `nextflow run nf-core/mag -profile eva`
## mag specific configurations for eva
Specific configurations for eva has been made for mag, primarily adjusting SGE memory requirements of Java tools (e.g. FastQC).

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@ -0,0 +1,19 @@
# nf-core/configs: eva taxprofiler specific configuration
Extra specific configuration for taxprofiler pipeline
## Usage
To use, run the pipeline with `-profile eva`.
This will download and launch the taxprofiler specific [`eva.config`](../../../conf/pipeline/taxprofiler/eva.config) which has been pre-configured with a setup suitable for the MPI-EVA cluster.
Example: `nextflow run nf-core/taxprofiler -profile eva`
## taxprofiler specific configurations for eva
Specific configurations for eva has been made for taxprofiler.
### General profiles
- The general MPI-EVA profile runs with default nf-core/taxprofiler parameters, but with modifications to account for issues SGE have with Java and python tools, nameling: BBDUK, MALT, MetaPhlAn3, and MEGAN

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@ -0,0 +1,19 @@
# nf-core/configs: eva taxprofiler specific configuration
Extra specific configuration for taxprofiler pipeline
## Usage
To use, run the pipeline with `-profile hasta`.
This will download and launch the taxprofiler specific [`hasta.config`](../../../conf/pipeline/taxprofiler/hasta.config) which has been pre-configured with a setup suitable for the hasta cluster.
Example: `nextflow run nf-core/taxprofiler -profile hasta`
## taxprofiler specific configurations for hasta
Specific configurations for hasta has been made for taxprofiler.
### General profiles
- The general hasta profile runs with default nf-core/taxprofiler parameters, but with modifications to account for issues with: BBDUK and MALT.

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@ -0,0 +1,27 @@
# nf-core/configs: Sage Bionetworks Global Configuration
To use this custom configuration, run the pipeline with `-profile sage`. This will download and load the [`sage.config`](../conf/sage.config), which contains a number of optimizations relevant to Sage employees running workflows on AWS (_e.g._ using Nextflow Tower). This profile will also load any applicable pipeline-specific configuration.
This global configuration includes the following tweaks:
- Update the default value for `igenomes_base` to `s3://sage-igenomes`
- Increase the default time limits because we run pipelines on AWS
- Enable retries by default when exit codes relate to insufficient memory
- Allow pending jobs to finish if the number of retries are exhausted
- Slow the increase in the number of allocated CPU cores on retries
- Define the `check_max()` function, which is missing in Sarek v2
## Additional information about iGenomes
The following iGenomes prefixes have been copied from `s3://ngi-igenomes/` (`eu-west-1`) to `s3://sage-igenomes` (`us-east-1`). See [this script](https://github.com/Sage-Bionetworks-Workflows/nextflow-infra/blob/main/bin/mirror-igenomes.sh) for more information. The `sage-igenomes` S3 bucket has been configured to openly available, but files cannot be downloaded out of `us-east-1` to avoid egress charges. You can check the `conf/igenomes.config` file in each nf-core pipeline to figure out the mapping between genome IDs (_i.e._ for `--genome`) and iGenomes prefixes ([example](https://github.com/nf-core/rnaseq/blob/89bf536ce4faa98b4d50a8ec0a0343780bc62e0a/conf/igenomes.config#L14-L26)).
- **Human Genome Builds**
- `Homo_sapiens/Ensembl/GRCh37`
- `Homo_sapiens/GATK/GRCh37`
- `Homo_sapiens/UCSC/hg19`
- `Homo_sapiens/GATK/GRCh38`
- `Homo_sapiens/NCBI/GRCh38`
- `Homo_sapiens/UCSC/hg38`
- **Mouse Genome Builds**
- `Mus_musculus/Ensembl/GRCm38`
- `Mus_musculus/UCSC/mm10`

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@ -2,8 +2,6 @@
To use, run the pipeline with `-profile sanger`. This will download and launch the [`sanger.config`](../conf/sanger.config) which has been To use, run the pipeline with `-profile sanger`. This will download and launch the [`sanger.config`](../conf/sanger.config) which has been
pre-configured with a setup suitable for the Wellcome Sanger Institute LSF cluster. pre-configured with a setup suitable for the Wellcome Sanger Institute LSF cluster.
Using this profile, either a docker image containing all of the required software will be downloaded, and converted to a Singularity image or
a Singularity image downloaded directly before execution of the pipeline.
## Running the workflow on the Wellcome Sanger Institute cluster ## Running the workflow on the Wellcome Sanger Institute cluster
@ -14,10 +12,12 @@ The latest version of Nextflow is not installed by default on the cluster. You w
A recommended place to move the `nextflow` executable to is `~/bin` so that it's in the `PATH`. A recommended place to move the `nextflow` executable to is `~/bin` so that it's in the `PATH`.
Nextflow manages each process as a separate job that is submitted to the cluster by using the `bsub` command. Nextflow manages each process as a separate job that is submitted to the cluster by using the `bsub` command.
Since the Nextflow pipeline will submit individual jobs for each process to the cluster and dependencies will be provided bu Singularity images you shoudl make sure that your account has access to the Singularity binary by adding these lines to your `.bashrc` file
If asking Nextflow to use Singularity to run the individual jobs,
you should make sure that your account has access to the Singularity binary by adding these lines to your `.bashrc` file
```bash ```bash
[[ -f /software/pathogen/farm5 ]] && module load ISG/singularity [[ -f /software/modules/ISG/singularity ]] && module load ISG/singularity
``` ```
Nextflow shouldn't run directly on the submission node but on a compute node. Nextflow shouldn't run directly on the submission node but on a compute node.
@ -26,16 +26,16 @@ To do so make a shell script with a similar structure to the following code and
```bash ```bash
#!/bin/bash #!/bin/bash
#BSUB -o /path/to/a/log/dir/%J.o #BSUB -o /path/to/a/log/dir/%J.o
#BSUB -e /path/to/a/log/dir//%J.e #BSUB -e /path/to/a/log/dir/%J.e
#BSUB -M 8000 #BSUB -M 8000
#BSUB -q long #BSUB -q oversubscribed
#BSUB -n 4 #BSUB -n 2
export HTTP_PROXY='http://wwwcache.sanger.ac.uk:3128' export HTTP_PROXY='http://wwwcache.sanger.ac.uk:3128'
export HTTPS_PROXY='http://wwwcache.sanger.ac.uk:3128' export HTTPS_PROXY='http://wwwcache.sanger.ac.uk:3128'
export NXF_ANSI_LOG=false export NXF_ANSI_LOG=false
export NXF_OPTS="-Xms8G -Xmx8G -Dnxf.pool.maxThreads=2000" export NXF_OPTS="-Xms8G -Xmx8G -Dnxf.pool.maxThreads=2000"
export NXF_VER=21.04.0-edge export NXF_VER=22.04.0-5697
nextflow run \ nextflow run \

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docs/vsc_ugent.md Normal file
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@ -0,0 +1,35 @@
# nf-core/configs: University of Ghent High Performance Computing Infrastructure (VSC)
> **NB:** You will need an [account](https://www.ugent.be/hpc/en/access/faq/access) to use the HPC cluster to run the pipeline.
First you should go to the cluster you want to run the pipeline on. You can check what clusters have the most free space on this [link](https://shieldon.ugent.be:8083/pbsmon-web-users/). Use the following commands to easily switch between clusters:
```shell
module purge
module swap cluster/<CLUSTER>
```
Before running the pipeline you will need to create a PBS script to submit as a job.
```bash
#!/bin/bash
module load Nextflow
nextflow run <pipeline> -profile vsc_ugent,<CLUSTER> <Add your other parameters>
```
All of the intermediate files required to run the pipeline will be stored in the `work/` directory. It is recommended to delete this directory after the pipeline has finished successfully because it can get quite large, and all of the main output files will be saved in the `results/` directory anyway.
The config contains a `cleanup` command that removes the `work/` directory automatically once the pipeline has completed successfully. If the run does not complete successfully then the `work/` dir should be removed manually to save storage space. The default work directory is set to `$VSC_SCRATCH_VO_USER/work` per this configuration
You can also add several TORQUE options to the PBS script. More about this on this [link](http://hpcugent.github.io/vsc_user_docs/pdf/intro-HPC-linux-gent.pdf#appendix.B).
To submit your job to the cluster by using the following command:
```shell
qsub <script name>.pbs
```
> **NB:** The profile only works for the clusters `skitty`, `swalot`, `victini`, `kirlia` and `doduo`.
> **NB:** The default directory where the `work/` and `singularity/` (cache directory for images) is located in `$VSC_SCRATCH_VO_USER`.

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@ -24,6 +24,7 @@ profiles {
cbe { includeConfig "${params.custom_config_base}/conf/cbe.config" } cbe { includeConfig "${params.custom_config_base}/conf/cbe.config" }
ccga_dx { includeConfig "${params.custom_config_base}/conf/ccga_dx.config" } ccga_dx { includeConfig "${params.custom_config_base}/conf/ccga_dx.config" }
ccga_med { includeConfig "${params.custom_config_base}/conf/ccga_med.config" } ccga_med { includeConfig "${params.custom_config_base}/conf/ccga_med.config" }
cedars { includeConfig "${params.custom_config_base}/conf/cedars.config" }
cfc { includeConfig "${params.custom_config_base}/conf/cfc.config" } cfc { includeConfig "${params.custom_config_base}/conf/cfc.config" }
cfc_dev { includeConfig "${params.custom_config_base}/conf/cfc_dev.config" } cfc_dev { includeConfig "${params.custom_config_base}/conf/cfc_dev.config" }
cheaha { includeConfig "${params.custom_config_base}/conf/cheaha.config" } cheaha { includeConfig "${params.custom_config_base}/conf/cheaha.config" }
@ -48,6 +49,8 @@ profiles {
lugh { includeConfig "${params.custom_config_base}/conf/lugh.config" } lugh { includeConfig "${params.custom_config_base}/conf/lugh.config" }
maestro { includeConfig "${params.custom_config_base}/conf/maestro.config" } maestro { includeConfig "${params.custom_config_base}/conf/maestro.config" }
marvin { includeConfig "${params.custom_config_base}/conf/marvin.config" } marvin { includeConfig "${params.custom_config_base}/conf/marvin.config" }
medair { includeConfig "${params.custom_config_base}/conf/medair.config" }
mjolnir_globe { includeConfig "${params.custom_config_base}/conf/mjolnir_globe.config" }
mpcdf { includeConfig "${params.custom_config_base}/conf/mpcdf.config" } mpcdf { includeConfig "${params.custom_config_base}/conf/mpcdf.config" }
munin { includeConfig "${params.custom_config_base}/conf/munin.config" } munin { includeConfig "${params.custom_config_base}/conf/munin.config" }
nihbiowulf { includeConfig "${params.custom_config_base}/conf/nihbiowulf.config" } nihbiowulf { includeConfig "${params.custom_config_base}/conf/nihbiowulf.config" }
@ -57,6 +60,7 @@ profiles {
phoenix { includeConfig "${params.custom_config_base}/conf/phoenix.config" } phoenix { includeConfig "${params.custom_config_base}/conf/phoenix.config" }
prince { includeConfig "${params.custom_config_base}/conf/prince.config" } prince { includeConfig "${params.custom_config_base}/conf/prince.config" }
rosalind { includeConfig "${params.custom_config_base}/conf/rosalind.config" } rosalind { includeConfig "${params.custom_config_base}/conf/rosalind.config" }
sage { includeConfig "${params.custom_config_base}/conf/sage.config" }
sahmri { includeConfig "${params.custom_config_base}/conf/sahmri.config" } sahmri { includeConfig "${params.custom_config_base}/conf/sahmri.config" }
sanger { includeConfig "${params.custom_config_base}/conf/sanger.config"} sanger { includeConfig "${params.custom_config_base}/conf/sanger.config"}
seg_globe { includeConfig "${params.custom_config_base}/conf/seg_globe.config"} seg_globe { includeConfig "${params.custom_config_base}/conf/seg_globe.config"}
@ -67,4 +71,5 @@ profiles {
utd_sysbio { includeConfig "${params.custom_config_base}/conf/utd_sysbio.config" } utd_sysbio { includeConfig "${params.custom_config_base}/conf/utd_sysbio.config" }
uzh { includeConfig "${params.custom_config_base}/conf/uzh.config" } uzh { includeConfig "${params.custom_config_base}/conf/uzh.config" }
vai { includeConfig "${params.custom_config_base}/conf/vai.config" } vai { includeConfig "${params.custom_config_base}/conf/vai.config" }
vsc_ugent { includeConfig "${params.custom_config_base}/conf/vsc_ugent.config" }
} }

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/*
* -------------------------------------------------
* nfcore/mag custom profile Nextflow config file
* -------------------------------------------------
* Config options for custom environments.
* Cluster-specific config options should be saved
* in the conf/pipeline/mag folder and imported
* under a profile name here.
*/
profiles {
eva { includeConfig "${params.custom_config_base}/conf/pipeline/mag/eva.config" }
}

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@ -15,4 +15,4 @@ profiles {
cfc { includeConfig "${params.custom_config_base}/conf/pipeline/sarek/cfc.config" } cfc { includeConfig "${params.custom_config_base}/conf/pipeline/sarek/cfc.config" }
cfc_dev { includeConfig "${params.custom_config_base}/conf/pipeline/sarek/cfc.config" } cfc_dev { includeConfig "${params.custom_config_base}/conf/pipeline/sarek/cfc.config" }
eddie { includeConfig "${params.custom_config_base}/conf/pipeline/sarek/eddie.config" } eddie { includeConfig "${params.custom_config_base}/conf/pipeline/sarek/eddie.config" }
} }

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/*
* -------------------------------------------------
* nfcore/taxprofiler custom profile Nextflow config file
* -------------------------------------------------
* Config options for custom environments.
* Cluster-specific config options should be saved
* in the conf/pipeline/taxprofiler folder and imported
* under a profile name here.
*/
profiles {
hasta { includeConfig "${params.custom_config_base}/conf/pipeline/taxprofiler/hasta.config" }
eva { includeConfig "${params.custom_config_base}/conf/pipeline/taxprofiler/eva.config" }
}