nf-core_modules/modules/qualimap/rnaseq/main.nf

process QUALIMAP_RNASEQ {
    tag "$meta.id"
    label 'process_medium'

    conda (params.enable_conda ? "bioconda::qualimap=2.2.2d" : null)
    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
        'https://depot.galaxyproject.org/singularity/qualimap:2.2.2d--1' :
        'quay.io/biocontainers/qualimap:2.2.2d--1' }"

    input:
    tuple val(meta), path(bam)
    path  gtf

    output:
    tuple val(meta), path("${prefix}"), emit: results
    path  "versions.yml"              , emit: versions

    when:
    task.ext.when == null || task.ext.when

    script:
    def args = task.ext.args   ?: ''
    prefix   = task.ext.prefix ?: "${meta.id}"
    def paired_end = meta.single_end ? '' : '-pe'
    def memory     = task.memory.toGiga() + "G"

    def strandedness = 'non-strand-specific'
    if (meta.strandedness == 'forward') {
        strandedness = 'strand-specific-forward'
    } else if (meta.strandedness == 'reverse') {
        strandedness = 'strand-specific-reverse'
    }
    """
    unset DISPLAY
    mkdir tmp
    export _JAVA_OPTIONS=-Djava.io.tmpdir=./tmp
    qualimap \\
        --java-mem-size=$memory \\
        rnaseq \\
        $args \\
        -bam $bam \\
        -gtf $gtf \\
        -p $strandedness \\
        $paired_end \\
        -outdir $prefix

    cat <<-END_VERSIONS > versions.yml
    "${task.process}":
        qualimap: \$(echo \$(qualimap 2>&1) | sed 's/^.*QualiMap v.//; s/Built.*\$//')
    END_VERSIONS
    """
}