nf-core_modules/modules/fgbio/groupreadsbyumi/meta.yml

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name: fgbio_groupreadsbyumi
description: |
Groups reads together that appear to have come from the same original molecule.
Reads are grouped by template, and then templates are sorted by the 5 mapping positions
of the reads from the template, used from earliest mapping position to latest.
Reads that have the same end positions are then sub-grouped by UMI sequence.
(!) Note: the MQ tag is required on reads with mapped mates (!)
This can be added using samblaster with the optional argument --addMateTags.
keywords:
- UMI
- groupreads
- fgbio
tools:
- fgbio:
description: A set of tools for working with genomic and high throughput sequencing data, including UMIs
homepage: http://fulcrumgenomics.github.io/fgbio/
documentation: http://fulcrumgenomics.github.io/fgbio/tools/latest/
tool_dev_url: https://github.com/fulcrumgenomics/fgbio
doi: ""
licence: ["MIT"]
input:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- bam:
type: file
description: |
BAM file. Note: the MQ tag is required on reads with mapped mates (!)
pattern: "*.bam"
- strategy:
type: value
description: |
Reguired argument: defines the UMI assignment strategy.
Must be chosen among: Identity, Edit, Adjacency, Paired.
output:
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
- bam:
type: file
description: UMI-grouped BAM
pattern: "*.bam"
- histogram:
type: file
description: A text file containing the tag family size counts
pattern: "*.txt"
authors:
- "@lescai"