nf-core_modules/modules/unicycler/main.nf

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// Import generic module functions
include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions'
params.options = [:]
options = initOptions(params.options)
process UNICYCLER {
tag "$meta.id"
label 'process_high'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
conda (params.enable_conda ? 'bioconda::unicycler=0.4.8' : null)
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
container "https://depot.galaxyproject.org/singularity/unicycler:0.4.8--py38h8162308_3"
} else {
container "quay.io/biocontainers/unicycler:0.4.8--py38h8162308_3"
}
input:
tuple val(meta), path(shortreads), path(longreads)
output:
tuple val(meta), path('*.scaffolds.fa.gz'), emit: scaffolds
tuple val(meta), path('*.assembly.gfa.gz'), emit: gfa
tuple val(meta), path('*.log') , emit: log
path "versions.yml" , emit: versions
script:
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
def short_reads = shortreads ? ( meta.single_end ? "-s $shortreads" : "-1 ${shortreads[0]} -2 ${shortreads[1]}" ) : ""
def long_reads = longreads ? "-l $longreads" : ""
"""
unicycler \\
--threads $task.cpus \\
$options.args \\
$short_reads \\
$long_reads \\
--out ./
mv assembly.fasta ${prefix}.scaffolds.fa
gzip -n ${prefix}.scaffolds.fa
mv assembly.gfa ${prefix}.assembly.gfa
gzip -n ${prefix}.assembly.gfa
mv unicycler.log ${prefix}.unicycler.log
cat <<-END_VERSIONS > versions.yml
${getProcessName(task.process)}:
${getSoftwareName(task.process)}: \$(echo \$(unicycler --version 2>&1) | sed 's/^.*Unicycler v//; s/ .*\$//')
END_VERSIONS
"""
}