nf-core_modules/modules/blast/tblastn/main.nf

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process BLAST_TBLASTN {
tag "$meta.id"
label 'process_medium'
conda (params.enable_conda ? 'bioconda::blast=2.12.0' : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/blast:2.12.0--pl5262h3289130_0' :
'quay.io/biocontainers/blast:2.12.0--pl5262h3289130_0' }"
input:
tuple val(meta), path(fasta)
path db
output:
tuple val(meta), path('*.tblastn.txt') , emit: txt
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
"""
DB=`find -L ./ -name "*.nsq" | sed 's/.nsq//'`
tblastn \\
-num_threads $task.cpus \\
-db \$DB \\
-query $fasta \\
$args \\
-out ${prefix}.tblastn.txt
cat <<-END_VERSIONS > versions.yml
"${task.process}":
blast: \$(tblastn -version 2>&1 | sed 's/^.*tblastn: //; s/ .*\$//')
END_VERSIONS
"""
}