2021-03-22 18:26:02 +00:00
|
|
|
// Import generic module functions
|
|
|
|
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
|
|
|
|
|
|
|
params.options = [:]
|
|
|
|
options = initOptions(params.options)
|
|
|
|
|
|
|
|
process GATK4_FASTQTOSAM {
|
|
|
|
tag "$meta.id"
|
|
|
|
label 'process_medium'
|
|
|
|
publishDir "${params.outdir}",
|
|
|
|
mode: params.publish_dir_mode,
|
2021-04-09 16:23:56 +00:00
|
|
|
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
|
2021-03-22 18:26:02 +00:00
|
|
|
|
|
|
|
conda (params.enable_conda ? "bioconda::gatk4=4.2.0.0" : null)
|
|
|
|
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
|
|
|
|
container "https://depot.galaxyproject.org/singularity/gatk4:4.2.0.0--0"
|
|
|
|
} else {
|
|
|
|
container "quay.io/biocontainers/gatk4:4.2.0.0--0"
|
|
|
|
}
|
|
|
|
|
|
|
|
input:
|
|
|
|
tuple val(meta), path(reads)
|
|
|
|
|
|
|
|
output:
|
|
|
|
tuple val(meta), path("*.bam"), emit: bam
|
|
|
|
path "*.version.txt" , emit: version
|
|
|
|
|
|
|
|
script:
|
|
|
|
def software = getSoftwareName(task.process)
|
|
|
|
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
|
|
|
|
def read_files = meta.single_end ? "-F1 $reads" : "-F1 ${reads[0]} -F2 ${reads[1]}"
|
|
|
|
"""
|
|
|
|
gatk FastqToSam \\
|
|
|
|
$read_files \\
|
|
|
|
-O ${prefix}.bam \\
|
|
|
|
-SM $prefix \\
|
|
|
|
$options.args
|
|
|
|
gatk --version | grep Picard | sed "s/Picard Version: //g" > ${software}.version.txt
|
|
|
|
"""
|
|
|
|
}
|