2020-12-17 23:50:24 +00:00
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process UMITOOLS_EXTRACT {
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tag "$meta.id"
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label "process_low"
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2021-07-16 13:21:08 +00:00
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conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
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2021-11-26 07:58:40 +00:00
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0' :
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'quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0' }"
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2020-12-17 23:50:24 +00:00
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input:
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tuple val(meta), path(reads)
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output:
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tuple val(meta), path("*.fastq.gz"), emit: reads
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tuple val(meta), path("*.log") , emit: log
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2021-10-01 13:04:56 +00:00
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path "versions.yml" , emit: versions
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2020-12-17 23:50:24 +00:00
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script:
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2021-11-26 07:58:40 +00:00
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def args = task.ext.args ?: ''
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def prefix = task.ext.suffix ? "${meta.id}${task.ext.suffix}" : "${meta.id}"
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2020-12-17 23:50:24 +00:00
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if (meta.single_end) {
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"""
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umi_tools \\
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extract \\
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-I $reads \\
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-S ${prefix}.umi_extract.fastq.gz \\
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2021-11-26 07:58:40 +00:00
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$args \\
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2020-12-17 23:50:24 +00:00
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> ${prefix}.umi_extract.log
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2021-09-27 08:41:24 +00:00
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cat <<-END_VERSIONS > versions.yml
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2021-11-26 07:58:40 +00:00
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"${task.process}":
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umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
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2021-09-27 08:41:24 +00:00
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END_VERSIONS
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2020-12-17 23:50:24 +00:00
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"""
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} else {
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"""
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umi_tools \\
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extract \\
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-I ${reads[0]} \\
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--read2-in=${reads[1]} \\
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-S ${prefix}.umi_extract_1.fastq.gz \\
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--read2-out=${prefix}.umi_extract_2.fastq.gz \\
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2021-11-26 07:58:40 +00:00
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$args \\
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2020-12-17 23:50:24 +00:00
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> ${prefix}.umi_extract.log
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2021-09-27 08:41:24 +00:00
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cat <<-END_VERSIONS > versions.yml
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2021-11-26 07:58:40 +00:00
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"${task.process}":
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umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
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2021-09-27 08:41:24 +00:00
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END_VERSIONS
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2020-12-17 23:50:24 +00:00
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"""
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}
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}
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