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48 lines
1.5 KiB
Text
48 lines
1.5 KiB
Text
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process SAMTOOLS_COLLATEFASTQ {
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tag "$meta.id"
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label 'process_low'
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conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
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'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
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input:
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tuple val(meta), path(input)
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output:
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//TODO might be good to have ordered output of the fastq files, so we can
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// make sure the we get the right files
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tuple val(meta), path("*_{1,2}.fq.gz"), path("*_other.fq.gz"), path("*_singleton.fq.gz"), emit: reads
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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def args2 = task.ext.args ?: ''
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def prefix = task.ext.prefix ?: "${meta.id}"
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"""
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samtools collate \\
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$args \\
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--threads $task.cpus \\
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-O \\
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$input \\
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. |
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samtools fastq \\
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$args2 \\
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--threads $task.cpus \\
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-1 ${prefix}_1.fq.gz \\
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-2 ${prefix}_2.fq.gz \\
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-0 ${prefix}_other.fq.gz \\
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-s ${prefix}_singleton.fq.gz
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
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END_VERSIONS
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"""
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}
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