2021-04-30 12:20:56 +00:00
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// Import generic module functions
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2021-09-27 08:41:24 +00:00
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include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions'
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2021-04-30 12:20:56 +00:00
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params.options = [:]
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2021-04-30 14:57:43 +00:00
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options = initOptions(params.options)
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2021-04-30 12:20:56 +00:00
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process QCAT {
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tag "$meta.id"
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label 'process_medium'
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
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2021-04-30 14:57:43 +00:00
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conda (params.enable_conda ? "bioconda::qcat=1.1.0" : null)
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if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
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container "https://depot.galaxyproject.org/singularity/qcat:1.1.0--py_0"
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} else {
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container "quay.io/biocontainers/qcat:1.1.0--py_0"
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}
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2021-04-30 12:20:56 +00:00
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input:
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tuple val(meta), path(reads)
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val barcode_kit
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output:
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tuple val(meta), path("fastq/*.fastq.gz"), emit: reads
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2021-10-01 13:04:56 +00:00
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path "versions.yml" , emit: versions
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2021-04-30 12:20:56 +00:00
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script:
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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2021-04-30 12:20:56 +00:00
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"""
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## Unzip fastq file
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## qcat doesn't support zipped files yet
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FILE=$reads
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if [[ \$FILE == *.gz ]]
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then
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zcat $reads > unzipped.fastq
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FILE=unzipped.fastq
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fi
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2021-04-30 14:57:43 +00:00
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qcat \\
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-f \$FILE \\
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-b ./fastq \\
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--kit $barcode_kit
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## Zip fastq files
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gzip fastq/*
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2021-09-27 08:41:24 +00:00
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cat <<-END_VERSIONS > versions.yml
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${getProcessName(task.process)}:
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${getSoftwareName(task.process)}: \$(qcat --version 2>&1 | sed 's/^.*qcat //; s/ .*\$//')
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END_VERSIONS
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2021-04-30 12:20:56 +00:00
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"""
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}
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