2021-03-22 17:16:04 +00:00
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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params.options = [:]
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options = initOptions(params.options)
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process ADAPTERREMOVAL {
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tag "$meta.id"
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label 'process_medium'
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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2021-04-09 16:23:56 +00:00
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saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
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2021-03-22 17:16:04 +00:00
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conda (params.enable_conda ? "bioconda::adapterremoval=2.3.2" : null)
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if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
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container "https://depot.galaxyproject.org/singularity/adapterremoval:2.3.2--hb7ba0dd_0"
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} else {
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container "quay.io/biocontainers/adapterremoval:2.3.2--hb7ba0dd_0"
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}
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input:
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tuple val(meta), path(reads)
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output:
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tuple val(meta), path('*.fastq.gz'), emit: reads
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tuple val(meta), path('*.log') , emit: log
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path "*.version.txt" , emit: version
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script:
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def software = getSoftwareName(task.process)
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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if (meta.single_end) {
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"""
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AdapterRemoval \\
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--file1 $reads \\
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$options.args \\
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--basename $prefix \\
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--threads $task.cpus \\
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--settings ${prefix}.log \\
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--output1 ${prefix}.trimmed.fastq.gz \\
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--seed 42 \\
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--gzip \\
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AdapterRemoval --version 2>&1 | sed -e "s/AdapterRemoval ver. //g" > ${software}.version.txt
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"""
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} else if (!meta.single_end && !meta.collapse) {
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"""
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AdapterRemoval \\
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--file1 ${reads[0]} \\
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--file2 ${reads[0]} \\
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$options.args \\
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--basename $prefix \\
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--threads $task.cpus \\
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--settings ${prefix}.log \\
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--output1 ${prefix}.pair1.trimmed.fastq.gz \\
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--output2 ${prefix}.pair2.trimmed.fastq.gz \\
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--seed 42 \\
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--gzip \\
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AdapterRemoval --version 2>&1 | sed -e "s/AdapterRemoval ver. //g" > ${software}.version.txt
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"""
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} else {
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"""
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AdapterRemoval \\
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--file1 ${reads[0]} \\
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--file2 ${reads[0]} \\
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--collapse \\
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$options.args \\
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--basename $prefix \\
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--threads $task.cpus \\
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--settings ${prefix}.log \\
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--seed 42 \\
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--gzip \\
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cat *.collapsed.gz *.collapsed.truncated.gz > ${prefix}.merged.fastq.gz
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AdapterRemoval --version 2>&1 | sed -e "s/AdapterRemoval ver. //g" > ${software}.version.txt
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"""
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}
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}
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