nf-core_modules/software/salmon/quant/main.nf

50 lines
1.5 KiB
Text
Raw Normal View History

2020-09-10 15:45:11 +00:00
// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'
2020-10-14 17:29:50 +00:00
params.options = [:]
def options = initOptions(params.options)
2020-09-10 15:45:11 +00:00
process SALMON_QUANT {
tag "$meta.id"
label "process_medium"
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
2020-10-14 17:29:50 +00:00
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
2020-09-10 15:45:11 +00:00
2020-11-12 14:55:16 +00:00
conda (params.enable_conda ? "bioconda::salmon=1.3.0" : null)
container "quay.io/biocontainers/salmon:1.3.0--hf69c8f4_0"
2020-09-10 15:45:11 +00:00
input:
tuple val(meta), path(reads)
path index
path gtf
2020-10-14 17:29:50 +00:00
2020-09-10 15:45:11 +00:00
output:
tuple val(meta), path("${prefix}"), emit: results
path "*.version.txt" , emit: version
script:
def software = getSoftwareName(task.process)
2020-10-14 17:29:50 +00:00
prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
2020-09-10 15:45:11 +00:00
def endedness = meta.single_end ? "-r $reads" : "-1 ${reads[0]} -2 ${reads[1]}"
def strandedness = meta.single_end ? 'U' : 'IU'
if (meta.strandedness == 'forward') {
strandedness = meta.single_end ? 'SF' : 'ISF'
} else if (meta.strandedness == 'reverse') {
strandedness = meta.single_end ? 'SR' : 'ISR'
}
"""
salmon quant \\
--geneMap $gtf \\
--threads $task.cpus \\
--libType=$strandedness \\
--index $index \\
$endedness \\
2020-10-14 17:29:50 +00:00
$options.args \\
2020-09-10 15:45:11 +00:00
-o $prefix
salmon --version | sed -e "s/salmon //g" > ${software}.version.txt
"""
}