2020-08-05 11:09:25 -04:00
|
|
|
// Import generic module functions
|
2020-08-05 12:17:58 -04:00
|
|
|
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
2019-12-05 09:35:51 -05:00
|
|
|
|
2020-10-14 13:29:50 -04:00
|
|
|
params.options = [:]
|
2021-03-15 08:16:43 -04:00
|
|
|
options = initOptions(params.options)
|
2020-10-14 13:29:50 -04:00
|
|
|
|
2020-07-11 07:42:13 -04:00
|
|
|
process FASTQC {
|
2020-08-05 11:09:25 -04:00
|
|
|
tag "$meta.id"
|
|
|
|
label 'process_medium'
|
|
|
|
publishDir "${params.outdir}",
|
2020-07-15 07:00:10 -04:00
|
|
|
mode: params.publish_dir_mode,
|
2021-04-09 12:23:56 -04:00
|
|
|
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
|
2019-12-05 09:35:51 -05:00
|
|
|
|
2021-02-16 18:58:23 -05:00
|
|
|
conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null)
|
2020-12-13 18:41:53 -05:00
|
|
|
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
|
|
|
|
container "https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0"
|
|
|
|
} else {
|
|
|
|
container "quay.io/biocontainers/fastqc:0.11.9--0"
|
|
|
|
}
|
2020-12-17 18:50:24 -05:00
|
|
|
|
2020-07-15 07:00:10 -04:00
|
|
|
input:
|
2020-08-05 11:09:25 -04:00
|
|
|
tuple val(meta), path(reads)
|
2020-12-17 18:50:24 -05:00
|
|
|
|
2020-07-15 07:00:10 -04:00
|
|
|
output:
|
2020-08-05 11:09:25 -04:00
|
|
|
tuple val(meta), path("*.html"), emit: html
|
2020-09-10 11:45:11 -04:00
|
|
|
tuple val(meta), path("*.zip") , emit: zip
|
|
|
|
path "*.version.txt" , emit: version
|
2020-03-16 10:17:38 -04:00
|
|
|
|
2020-07-11 07:42:13 -04:00
|
|
|
script:
|
2020-07-15 07:00:10 -04:00
|
|
|
// Add soft-links to original FastQs for consistent naming in pipeline
|
2020-08-05 11:09:25 -04:00
|
|
|
def software = getSoftwareName(task.process)
|
2021-07-01 06:14:58 -04:00
|
|
|
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
|
2020-08-05 11:09:25 -04:00
|
|
|
if (meta.single_end) {
|
2020-07-15 07:00:10 -04:00
|
|
|
"""
|
2020-08-05 11:09:25 -04:00
|
|
|
[ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
|
2020-10-14 13:29:50 -04:00
|
|
|
fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz
|
2020-08-05 11:09:25 -04:00
|
|
|
fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt
|
2020-07-15 07:00:10 -04:00
|
|
|
"""
|
|
|
|
} else {
|
2020-07-11 07:42:13 -04:00
|
|
|
"""
|
2020-08-05 11:09:25 -04:00
|
|
|
[ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
|
|
|
|
[ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
|
2020-10-14 13:29:50 -04:00
|
|
|
fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz
|
2020-08-05 11:09:25 -04:00
|
|
|
fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt
|
2020-07-11 07:42:13 -04:00
|
|
|
"""
|
2020-07-15 07:00:10 -04:00
|
|
|
}
|
2019-12-05 09:35:51 -05:00
|
|
|
}
|