2021-07-20 20:31:31 +00:00
|
|
|
// Import generic module functions
|
2021-09-27 08:41:24 +00:00
|
|
|
include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions'
|
2021-07-20 20:31:31 +00:00
|
|
|
|
|
|
|
params.options = [:]
|
|
|
|
options = initOptions(params.options)
|
|
|
|
|
|
|
|
process SAMTOOLS_AMPLICONCLIP {
|
|
|
|
tag "$meta.id"
|
|
|
|
label 'process_medium'
|
|
|
|
publishDir "${params.outdir}",
|
|
|
|
mode: params.publish_dir_mode,
|
|
|
|
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
|
|
|
|
|
|
|
|
conda (params.enable_conda ? "bioconda::samtools=1.13" : null)
|
|
|
|
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
|
|
|
|
container "https://depot.galaxyproject.org/singularity/samtools:1.13--h8c37831_0"
|
|
|
|
} else {
|
|
|
|
container "quay.io/biocontainers/samtools:1.13--h8c37831_0"
|
|
|
|
}
|
|
|
|
|
|
|
|
input:
|
|
|
|
tuple val(meta), path(bam)
|
|
|
|
path bed
|
|
|
|
val save_cliprejects
|
|
|
|
val save_clipstats
|
|
|
|
|
|
|
|
output:
|
|
|
|
tuple val(meta), path("*.bam") , emit: bam
|
|
|
|
tuple val(meta), path("*.clipstats.txt") , optional:true, emit: stats
|
|
|
|
tuple val(meta), path("*.cliprejects.bam"), optional:true, emit: rejects_bam
|
2021-09-27 08:41:24 +00:00
|
|
|
path "versions.yml" , emit: version
|
2021-07-20 20:31:31 +00:00
|
|
|
|
|
|
|
script:
|
|
|
|
def software = getSoftwareName(task.process)
|
|
|
|
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
|
|
|
|
def rejects = save_cliprejects ? "--rejects-file ${prefix}.cliprejects.bam" : ""
|
|
|
|
def stats = save_clipstats ? "-f ${prefix}.clipstats.txt" : ""
|
|
|
|
"""
|
|
|
|
samtools \\
|
|
|
|
ampliconclip \\
|
|
|
|
$options.args \\
|
|
|
|
-@ $task.cpus \\
|
|
|
|
$rejects \\
|
|
|
|
$stats \\
|
|
|
|
-b $bed \\
|
|
|
|
-o ${prefix}.bam \\
|
|
|
|
$bam
|
|
|
|
|
2021-09-27 08:41:24 +00:00
|
|
|
cat <<-END_VERSIONS > versions.yml
|
|
|
|
${getProcessName(task.process)}:
|
|
|
|
${getSoftwareName(task.process)}: \$(samtools --version 2>&1 | sed 's/^.*samtools //; s/Using.*\$//')
|
|
|
|
END_VERSIONS
|
2021-07-20 20:31:31 +00:00
|
|
|
"""
|
|
|
|
}
|