2020-08-05 15:09:25 +00:00
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// Import generic module functions
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2020-08-05 16:17:58 +00:00
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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2019-12-05 14:35:51 +00:00
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2020-10-14 17:29:50 +00:00
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params.options = [:]
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def options = initOptions(params.options)
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2020-07-11 11:42:13 +00:00
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process FASTQC {
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2020-08-05 15:09:25 +00:00
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tag "$meta.id"
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label 'process_medium'
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publishDir "${params.outdir}",
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2020-07-15 11:00:10 +00:00
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mode: params.publish_dir_mode,
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2020-10-14 17:29:50 +00:00
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saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
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2019-12-05 14:35:51 +00:00
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2020-11-12 14:55:16 +00:00
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conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null)
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container "quay.io/biocontainers/fastqc:0.11.9--0"
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2020-10-14 17:29:50 +00:00
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2020-07-15 11:00:10 +00:00
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input:
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2020-08-05 15:09:25 +00:00
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tuple val(meta), path(reads)
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2020-10-14 17:29:50 +00:00
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2020-07-15 11:00:10 +00:00
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output:
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2020-08-05 15:09:25 +00:00
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tuple val(meta), path("*.html"), emit: html
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2020-09-10 15:45:11 +00:00
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tuple val(meta), path("*.zip") , emit: zip
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path "*.version.txt" , emit: version
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2020-03-16 14:17:38 +00:00
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2020-07-11 11:42:13 +00:00
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script:
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2020-07-15 11:00:10 +00:00
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// Add soft-links to original FastQs for consistent naming in pipeline
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2020-08-05 15:09:25 +00:00
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def software = getSoftwareName(task.process)
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2020-10-14 17:29:50 +00:00
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def prefix = options.suffix ? "${meta.id}.${options.suffix}" : "${meta.id}"
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2020-08-05 15:09:25 +00:00
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if (meta.single_end) {
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2020-07-15 11:00:10 +00:00
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"""
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2020-08-05 15:09:25 +00:00
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[ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
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2020-10-14 17:29:50 +00:00
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fastqc $options.args --threads $task.cpus ${prefix}.fastq.gz
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2020-08-05 15:09:25 +00:00
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fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt
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2020-07-15 11:00:10 +00:00
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"""
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} else {
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2020-07-11 11:42:13 +00:00
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"""
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2020-08-05 15:09:25 +00:00
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[ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
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[ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
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2020-10-14 17:29:50 +00:00
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fastqc $options.args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz
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2020-08-05 15:09:25 +00:00
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fastqc --version | sed -e "s/FastQC v//g" > ${software}.version.txt
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2020-07-11 11:42:13 +00:00
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"""
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2020-07-15 11:00:10 +00:00
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}
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2019-12-05 14:35:51 +00:00
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}
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