nf-core_modules/modules/picard/fastqtosam/main.nf

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process PICARD_FASTQTOSAM {
tag "$meta.id"
label 'process_medium'
conda (params.enable_conda ? "bioconda::picard=2.27.4" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/picard:2.27.4--hdfd78af_0' :
'quay.io/biocontainers/picard:2.27.4--hdfd78af_0' }"
input:
tuple val(meta), path(reads)
output:
tuple val(meta), path("*.bam"), emit: bam
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
if (!task.memory) {
log.warn '[Picard FastqToSam] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
}
def avail_mem = task.memory ? task.memory.giga : 3
def input = meta.single_end ? "--FASTQ ${reads}" : "--FASTQ ${reads[0]} --FASTQ2 ${reads[1]}"
def sample_name = args.contains("--SAMPLE_NAME") || args.contains("-SM") ? "" : "--SAMPLE_NAME ${prefix}"
"""
picard \\
-Xmx${avail_mem}g \\
FastqToSam \\
${args} \\
${input} \\
${sample_name} \\
--OUTPUT ${prefix}.bam
cat <<-END_VERSIONS > versions.yml
"${task.process}":
picard: \$(picard FastqToSam --version 2>&1 | grep -o 'Version:.*' | cut -f2- -d:)
END_VERSIONS
"""
}