2021-07-27 03:13:48 -04:00
|
|
|
// Import generic module functions
|
|
|
|
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
|
|
|
|
|
|
|
params.options = [:]
|
|
|
|
options = initOptions(params.options)
|
|
|
|
|
|
|
|
process BWA_ALN {
|
|
|
|
tag "$meta.id"
|
|
|
|
label 'process_medium'
|
|
|
|
publishDir "${params.outdir}",
|
|
|
|
mode: params.publish_dir_mode,
|
|
|
|
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
|
|
|
|
|
|
|
|
conda (params.enable_conda ? "bioconda::bwa=0.7.17" : null)
|
|
|
|
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
|
|
|
|
container "https://depot.galaxyproject.org/singularity/bwa:0.7.17--h5bf99c6_8"
|
|
|
|
} else {
|
|
|
|
container "quay.io/biocontainers/bwa:0.7.17--h5bf99c6_8"
|
|
|
|
}
|
|
|
|
|
|
|
|
input:
|
|
|
|
tuple val(meta), path(reads)
|
|
|
|
path index
|
|
|
|
|
|
|
|
output:
|
2021-08-17 09:55:54 -04:00
|
|
|
tuple val(meta), path("*.sai"), emit: sai
|
2021-07-27 03:13:48 -04:00
|
|
|
path "*.version.txt" , emit: version
|
|
|
|
|
|
|
|
script:
|
|
|
|
def software = getSoftwareName(task.process)
|
|
|
|
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
|
|
|
|
|
|
|
|
if (meta.single_end) {
|
|
|
|
"""
|
|
|
|
INDEX=`find -L ./ -name "*.amb" | sed 's/.amb//'`
|
|
|
|
|
|
|
|
bwa aln \\
|
|
|
|
$options.args \\
|
|
|
|
-t $task.cpus \\
|
|
|
|
-f ${prefix}.sai \\
|
|
|
|
\$INDEX \\
|
|
|
|
${reads}
|
|
|
|
|
|
|
|
echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//' > ${software}.version.txt
|
|
|
|
"""
|
|
|
|
} else {
|
|
|
|
"""
|
|
|
|
INDEX=`find -L ./ -name "*.amb" | sed 's/.amb//'`
|
|
|
|
|
|
|
|
bwa aln \\
|
|
|
|
$options.args \\
|
|
|
|
-t $task.cpus \\
|
|
|
|
-f ${prefix}.1.sai \\
|
|
|
|
\$INDEX \\
|
|
|
|
${reads[0]}
|
|
|
|
|
|
|
|
bwa aln \\
|
|
|
|
$options.args \\
|
|
|
|
-t $task.cpus \\
|
|
|
|
-f ${prefix}.2.sai \\
|
|
|
|
\$INDEX \\
|
|
|
|
${reads[1]}
|
|
|
|
|
|
|
|
echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//' > ${software}.version.txt
|
|
|
|
"""
|
|
|
|
}
|
|
|
|
}
|