2021-03-23 11:13:07 +00:00
|
|
|
// Import generic module functions
|
|
|
|
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
|
|
|
|
|
|
|
params.options = [:]
|
|
|
|
options = initOptions(params.options)
|
|
|
|
|
|
|
|
process SAMTOOLS_FASTQ {
|
|
|
|
tag "$meta.id"
|
|
|
|
label 'process_low'
|
|
|
|
publishDir "${params.outdir}",
|
|
|
|
mode: params.publish_dir_mode,
|
|
|
|
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
|
|
|
|
|
|
|
|
conda (params.enable_conda ? "bioconda::samtools=1.12" : null)
|
|
|
|
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
|
|
|
|
container "https://depot.galaxyproject.org/singularity/samtools:1.12--hd5e65b6_0"
|
|
|
|
} else {
|
|
|
|
container "quay.io/biocontainers/samtools:1.12--hd5e65b6_0"
|
|
|
|
}
|
|
|
|
|
|
|
|
input:
|
|
|
|
tuple val(meta), path(bam)
|
|
|
|
|
|
|
|
output:
|
2021-03-23 17:43:52 +00:00
|
|
|
tuple val(meta), path("*.fastq.gz"), emit: fastq
|
2021-03-23 11:13:07 +00:00
|
|
|
path "*.version.txt" , emit: version
|
|
|
|
|
|
|
|
script:
|
|
|
|
def software = getSoftwareName(task.process)
|
2021-03-23 17:43:52 +00:00
|
|
|
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
|
|
|
|
def endedness = meta.single_end ? "-0 ${prefix}.fastq.gz" : "-1 ${prefix}_1.fastq.gz -2 ${prefix}_2.fastq.gz"
|
|
|
|
|
2021-03-23 11:13:07 +00:00
|
|
|
"""
|
2021-03-23 17:43:52 +00:00
|
|
|
samtools fastq \\
|
2021-03-23 11:13:07 +00:00
|
|
|
$options.args \\
|
|
|
|
-@ $task.cpus \\
|
2021-03-23 17:43:52 +00:00
|
|
|
$endedness \\
|
|
|
|
$bam
|
2021-03-23 11:13:07 +00:00
|
|
|
echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt
|
|
|
|
"""
|
|
|
|
}
|