2021-07-06 12:40:55 +00:00
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// Import generic module functions
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2021-09-27 08:41:24 +00:00
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include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions'
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2021-07-06 12:40:55 +00:00
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params.options = [:]
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options = initOptions(params.options)
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params.use_cache = false
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params.snpeff_tag = ""
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process SNPEFF {
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label 'process_medium'
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
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conda (params.enable_conda ? "bioconda::snpeff=5.0" : null)
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if (params.use_cache) {
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if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
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container "https://depot.galaxyproject.org/singularity/snpeff:5.0--hdfd78af_1"
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} else {
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container "quay.io/biocontainers/snpeff:5.0--hdfd78af_1"
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}
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} else {
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container "nfcore/snpeff:${params.snpeff_tag}"
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}
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input:
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tuple val(meta), path(vcf)
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val db
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path cache
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output:
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tuple val(meta), path("*.ann.vcf"), emit: vcf
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path "*.csv" , emit: report
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2021-10-01 13:04:56 +00:00
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path "versions.yml" , emit: versions
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2021-07-06 12:40:55 +00:00
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script:
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def avail_mem = 6
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if (!task.memory) {
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log.info '[snpEff] Available memory not known - defaulting to 6GB. Specify process memory requirements to change this.'
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} else {
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avail_mem = task.memory.giga
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}
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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cache = params.use_cache ? "-dataDir \${PWD}/${snpeff_cache}" : ""
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"""
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snpEff -Xmx${avail_mem}g \\
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$db \\
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$options.args \\
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-csvStats ${prefix}.csv \\
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$cache \\
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$vcf \\
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> ${prefix}.ann.vcf
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2021-09-27 08:41:24 +00:00
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cat <<-END_VERSIONS > versions.yml
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${getProcessName(task.process)}:
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2021-09-28 09:51:19 +00:00
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${getSoftwareName(task.process)}: \$(echo \$(snpEff -version 2>&1) | cut -f 2 -d ' ')
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2021-09-27 08:41:24 +00:00
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END_VERSIONS
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2021-07-06 12:40:55 +00:00
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"""
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}
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