2020-09-10 15:45:11 +00:00
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// Import generic module functions
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2021-09-27 08:41:24 +00:00
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include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions'
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2020-09-10 15:45:11 +00:00
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2020-10-14 17:59:25 +00:00
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params.options = [:]
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2021-03-15 12:16:43 +00:00
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options = initOptions(params.options)
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2020-10-14 17:59:25 +00:00
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2020-09-10 15:45:11 +00:00
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process PICARD_MARKDUPLICATES {
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tag "$meta.id"
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label 'process_medium'
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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2021-04-09 16:23:56 +00:00
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saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
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2020-12-17 23:50:24 +00:00
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2021-07-28 08:10:44 +00:00
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conda (params.enable_conda ? 'bioconda::picard=2.25.7' : null)
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2020-12-13 23:41:53 +00:00
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if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
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2021-07-28 08:10:44 +00:00
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container "https://depot.galaxyproject.org/singularity/picard:2.25.7--hdfd78af_0"
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2020-12-13 23:41:53 +00:00
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} else {
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2021-07-28 08:10:44 +00:00
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container "quay.io/biocontainers/picard:2.25.7--hdfd78af_0"
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2020-12-13 23:41:53 +00:00
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}
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2020-09-10 15:45:11 +00:00
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input:
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tuple val(meta), path(bam)
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2020-12-17 23:50:24 +00:00
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2020-09-10 15:45:11 +00:00
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output:
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tuple val(meta), path("*.bam") , emit: bam
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2021-09-15 09:02:28 +00:00
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tuple val(meta), path("*.bai") , optional:true, emit: bai
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2020-09-10 15:45:11 +00:00
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tuple val(meta), path("*.metrics.txt"), emit: metrics
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2021-10-01 13:04:56 +00:00
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path "versions.yml" , emit: versions
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2020-09-10 15:45:11 +00:00
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script:
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2020-10-14 17:59:25 +00:00
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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2020-09-10 15:45:11 +00:00
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def avail_mem = 3
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if (!task.memory) {
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log.info '[Picard MarkDuplicates] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
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} else {
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avail_mem = task.memory.giga
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}
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"""
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picard \\
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-Xmx${avail_mem}g \\
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MarkDuplicates \\
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2020-10-14 17:59:25 +00:00
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$options.args \\
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2021-09-15 09:02:28 +00:00
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-I $bam \\
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-O ${prefix}.bam \\
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-M ${prefix}.MarkDuplicates.metrics.txt
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2020-09-10 15:45:11 +00:00
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2021-09-27 08:41:24 +00:00
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cat <<-END_VERSIONS > versions.yml
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${getProcessName(task.process)}:
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2021-09-28 09:51:19 +00:00
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${getSoftwareName(task.process)}: \$(echo \$(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:)
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2021-09-27 08:41:24 +00:00
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END_VERSIONS
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2020-09-10 15:45:11 +00:00
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"""
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}
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