2021-03-24 05:07:29 +00:00
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process STRELKA_GERMLINE {
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tag "$meta.id"
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2022-09-07 08:33:05 +00:00
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label 'process_medium'
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2021-03-24 05:07:29 +00:00
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conda (params.enable_conda ? "bioconda::strelka=2.9.10" : null)
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2021-11-26 07:58:40 +00:00
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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2022-07-21 09:44:07 +00:00
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'https://depot.galaxyproject.org/singularity/strelka:2.9.10--h9ee0642_1' :
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'quay.io/biocontainers/strelka:2.9.10--h9ee0642_1' }"
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2021-03-24 05:07:29 +00:00
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input:
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2022-02-10 09:34:22 +00:00
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tuple val(meta), path(input), path(input_index), path (target_bed), path (target_bed_tbi)
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2021-03-24 09:53:41 +00:00
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path fasta
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path fai
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2021-10-27 14:14:52 +00:00
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2021-03-24 05:07:29 +00:00
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output:
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2021-07-01 15:13:01 +00:00
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tuple val(meta), path("*variants.vcf.gz") , emit: vcf
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tuple val(meta), path("*variants.vcf.gz.tbi"), emit: vcf_tbi
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tuple val(meta), path("*genome.vcf.gz") , emit: genome_vcf
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tuple val(meta), path("*genome.vcf.gz.tbi") , emit: genome_vcf_tbi
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2021-10-27 14:14:52 +00:00
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path "versions.yml" , emit: versions
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2021-03-24 05:07:29 +00:00
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2022-02-04 08:53:32 +00:00
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when:
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task.ext.when == null || task.ext.when
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2021-03-24 05:07:29 +00:00
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script:
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2021-11-26 07:58:40 +00:00
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def args = task.ext.args ?: ''
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2021-12-02 12:39:55 +00:00
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def prefix = task.ext.prefix ?: "${meta.id}"
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2022-02-10 09:34:22 +00:00
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def regions = target_bed ? "--callRegions ${target_bed}" : ""
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2021-03-24 05:07:29 +00:00
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"""
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2021-03-24 09:53:41 +00:00
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configureStrelkaGermlineWorkflow.py \\
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2021-10-29 11:01:05 +00:00
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--bam $input \\
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2021-03-24 09:53:41 +00:00
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--referenceFasta $fasta \\
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2021-07-01 15:13:01 +00:00
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$regions \\
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2021-11-26 07:58:40 +00:00
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$args \\
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2021-03-24 05:07:29 +00:00
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--runDir strelka
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2021-03-24 09:53:41 +00:00
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python strelka/runWorkflow.py -m local -j $task.cpus
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2021-07-01 15:13:01 +00:00
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mv strelka/results/variants/genome.*.vcf.gz ${prefix}.genome.vcf.gz
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mv strelka/results/variants/genome.*.vcf.gz.tbi ${prefix}.genome.vcf.gz.tbi
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mv strelka/results/variants/variants.vcf.gz ${prefix}.variants.vcf.gz
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mv strelka/results/variants/variants.vcf.gz.tbi ${prefix}.variants.vcf.gz.tbi
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2021-03-24 09:53:41 +00:00
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2021-09-27 08:41:24 +00:00
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cat <<-END_VERSIONS > versions.yml
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2021-11-26 07:58:40 +00:00
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"${task.process}":
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strelka: \$( configureStrelkaGermlineWorkflow.py --version )
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2021-09-27 08:41:24 +00:00
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END_VERSIONS
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2021-03-24 05:07:29 +00:00
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"""
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}
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