nf-core_modules/software/bwameth/align/main.nf

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// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'
params.options = [:]
options = initOptions(params.options)
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process BWAMETH_ALIGN {
tag "$meta.id"
label 'process_high'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
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conda (params.enable_conda ? "bioconda::bwameth=0.2.2" : null)
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
container "https://depot.galaxyproject.org/singularity/bwameth:0.2.2--py_1"
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} else {
container "quay.io/biocontainers/bwameth:0.2.2--py_1"
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}
input:
tuple val(meta), path(reads)
path index
output:
tuple val(meta), path("*.bam"), emit: bam
path "*.version.txt" , emit: version
script:
def split_cpus = Math.floor(task.cpus/2)
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def software = getSoftwareName(task.process)
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
def read_group = meta.read_group ? "-R ${meta.read_group}" : ""
"""
INDEX=`find -L ${index} -name "*.bwameth.c2t" | sed 's/.bwameth.c2t//'`
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bwameth.py \\
$options.args \\
$read_group \\
-t ${split_cpus} \\
--reference \$INDEX \\
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$reads \\
| samtools view $options.args2 -@ ${split_cpus} -bhS -o ${prefix}.bam -
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echo \$(bwameth.py --version 2>&1) | cut -f2 -d" " > ${software}.version.txt
"""
}