2021-05-10 10:45:52 +00:00
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process BBMAP_BBDUK {
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tag "$meta.id"
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label 'process_medium'
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conda (params.enable_conda ? "bioconda::bbmap=38.90" : null)
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2021-11-26 07:58:40 +00:00
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/bbmap:38.90--he522d1c_1' :
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'quay.io/biocontainers/bbmap:38.90--he522d1c_1' }"
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2021-05-10 10:45:52 +00:00
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input:
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tuple val(meta), path(reads)
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path contaminants
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output:
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tuple val(meta), path('*.fastq.gz'), emit: reads
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tuple val(meta), path('*.log') , emit: log
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2021-10-01 13:04:56 +00:00
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path "versions.yml" , emit: versions
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2022-02-04 08:53:32 +00:00
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when:
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task.ext.when == null || task.ext.when
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2021-05-10 10:45:52 +00:00
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script:
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2021-11-26 07:58:40 +00:00
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def args = task.ext.args ?: ''
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2021-12-02 12:39:55 +00:00
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def prefix = task.ext.prefix ?: "${meta.id}"
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2021-05-10 10:45:52 +00:00
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def raw = meta.single_end ? "in=${reads[0]}" : "in1=${reads[0]} in2=${reads[1]}"
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def trimmed = meta.single_end ? "out=${prefix}.fastq.gz" : "out1=${prefix}_1.fastq.gz out2=${prefix}_2.fastq.gz"
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def contaminants_fa = contaminants ? "ref=$contaminants" : ''
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"""
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maxmem=\$(echo \"$task.memory\"| sed 's/ GB/g/g')
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bbduk.sh \\
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-Xmx\$maxmem \\
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$raw \\
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$trimmed \\
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threads=$task.cpus \\
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2021-11-26 07:58:40 +00:00
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$args \\
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2021-05-10 10:45:52 +00:00
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$contaminants_fa \\
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&> ${prefix}.bbduk.log
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2021-09-27 08:41:24 +00:00
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cat <<-END_VERSIONS > versions.yml
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2021-11-26 07:58:40 +00:00
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"${task.process}":
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bbmap: \$(bbversion.sh)
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2021-09-27 08:41:24 +00:00
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END_VERSIONS
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2021-05-10 10:45:52 +00:00
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"""
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}
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