mirror of
https://github.com/MillironX/nf-core_modules.git
synced 2024-11-15 22:23:09 +00:00
34 lines
1.1 KiB
Text
34 lines
1.1 KiB
Text
|
#!/usr/bin/env nextflow
|
||
|
|
||
|
nextflow.enable.dsl = 2
|
||
|
|
||
|
include { CELLRANGER_MKGTF } from '../../../../modules/cellranger/mkgtf/main.nf'
|
||
|
include { CELLRANGER_MKREF } from '../../../../modules/cellranger/mkref/main.nf'
|
||
|
include { CELLRANGER_COUNT } from '../../../../modules/cellranger/count/main.nf'
|
||
|
|
||
|
workflow test_cellranger_count {
|
||
|
|
||
|
input = [ [ id:'test', single_end:true, strandedness:'forward', gem: '123', samples: ["test_10x"] ], // meta map
|
||
|
[ file(params.test_data['homo_sapiens']['illumina']['test_10x_1_fastq_gz'], checkIfExists: true),
|
||
|
file(params.test_data['homo_sapiens']['illumina']['test_10x_2_fastq_gz'], checkIfExists: true)
|
||
|
]
|
||
|
]
|
||
|
|
||
|
fasta = file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)
|
||
|
gtf = file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)
|
||
|
reference_name = "homo_sapiens_chr22_reference"
|
||
|
|
||
|
CELLRANGER_MKGTF ( gtf )
|
||
|
|
||
|
CELLRANGER_MKREF (
|
||
|
fasta,
|
||
|
CELLRANGER_MKGTF.out.gtf,
|
||
|
reference_name
|
||
|
)
|
||
|
|
||
|
CELLRANGER_COUNT(
|
||
|
input,
|
||
|
CELLRANGER_MKREF.out.reference
|
||
|
)
|
||
|
}
|