2022-04-27 09:12:58 +00:00
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process VARDICTJAVA {
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tag "$meta.id"
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label 'process_medium'
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conda (params.enable_conda ? "bioconda::vardict-java=1.8.3" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/vardict-java:1.8.3--hdfd78af_0':
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'quay.io/biocontainers/vardict-java:1.8.3--hdfd78af_0' }"
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input:
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2022-04-28 08:23:07 +00:00
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tuple val(meta), path(bam), path(bai)
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2022-04-28 07:18:15 +00:00
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path(regions_of_interest)
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2022-04-28 08:23:07 +00:00
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tuple path(reference_fasta), path(reference_fai)
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output:
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2022-04-28 08:23:07 +00:00
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tuple val(meta), path("*.vcf.gz"), emit: vcf
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2022-04-28 12:29:37 +00:00
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path "versions.yml" , emit: versions
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2022-04-27 09:12:58 +00:00
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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2022-04-28 13:33:27 +00:00
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def args_conversion = task.ext.args_conversion ?: ''
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2022-04-27 09:12:58 +00:00
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def prefix = task.ext.prefix ?: "${meta.id}"
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2022-04-27 09:18:58 +00:00
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2022-04-27 09:12:58 +00:00
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"""
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vardict-java \\
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$args \\
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2022-04-28 13:33:27 +00:00
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-c 1 -S 2 -E 3 \\
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2022-04-27 09:12:58 +00:00
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-b $bam \\
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-th $task.cpus \\
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2022-04-28 07:18:15 +00:00
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-N $prefix \\
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-G $reference_fasta \\
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2022-04-28 11:31:37 +00:00
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$regions_of_interest \\
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2022-04-28 13:33:27 +00:00
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| teststrandbias.R \\
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| var2vcf_valid.pl \\
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$args_conversion \\
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-N $prefix \\
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2022-04-28 07:28:57 +00:00
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| gzip -c > ${prefix}.vcf.gz
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2022-04-27 09:12:58 +00:00
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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2022-04-28 07:07:52 +00:00
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vardict-java: \$(echo 1.8.3)
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2022-04-27 09:12:58 +00:00
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END_VERSIONS
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"""
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}
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