nf-core_modules/software/seqkit/split2/main.nf

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2021-02-17 16:09:24 +00:00
// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'
// TODO nf-core: If in doubt look at other nf-core/modules to see how we are doing things! :)
// https://github.com/nf-core/modules/tree/master/software
// You can also ask for help via your pull request or on the #modules channel on the nf-core Slack workspace:
// https://nf-co.re/join
// TODO nf-core: The key words "MUST", "MUST NOT", "SHOULD", etc. are to be interpreted as described in RFC 2119 (https://tools.ietf.org/html/rfc2119).
// TODO nf-core: A module file SHOULD only define input and output files as command-line parameters.
// All other parameters MUST be provided as a string i.e. "options.args"
// where "params.options" is a Groovy Map that MUST be provided via the addParams section of the including workflow.
// Any parameters that need to be evaluated in the context of a particular sample
// e.g. single-end/paired-end data MUST also be defined and evaluated appropriately.
// TODO nf-core: Software that can be piped together SHOULD be added to separate module files
// unless there is a run-time, storage advantage in implementing in this way
// e.g. bwa mem | samtools view -B -T ref.fasta to output BAM instead of SAM.
// TODO nf-core: Optional inputs are not currently supported by Nextflow. However, "fake files" MAY be used to work around this issue.
params.options = [:]
def options = initOptions(params.options)
process SEQKIT_SPLIT2 {
tag "$meta.id"
label 'process_medium'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
conda (params.enable_conda ? "bioconda::seqkit=0.15.0" : null)
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
container "https://depot.galaxyproject.org/singularity/seqkit:0.15.0--0"
} else {
container "quay.io/biocontainers/seqkit:0.15.0--0"
}
input:
tuple val(meta), path(read1), path(read2)
// TODO nf-core: Where applicable all sample-specific information e.g. "id", "single_end", "read_group"
// MUST be provided as an input via a Groovy Map called "meta".
// This information may not be required in some instances e.g. indexing reference genome files:
// https://github.com/nf-core/modules/blob/master/software/bwa/index/main.nf
// TODO nf-core: Where applicable please provide/convert compressed files as input/output
// e.g. "*.fastq.gz" and NOT "*.fastq", "*.bam" and NOT "*.sam" etc.
output:
tuple val(meta), path("*.fq.gz"), emit: reads
path("*.version.txt") , emit: version
script:
def software = getSoftwareName(task.process)
//TODO not sure if this is useful here, as the splits need to be named individually, and this would make the prefix the same and the outputname I am afraid.
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
if(meta.single_end){ //TODO: I guess since optionally inputs are not possible right now, we need two modules, one for single_end and one for paired_end
"""
seqkit \
split2 \
$options.args \
--threads $task.cpus \
-1 $read1 \
-O $prefix
echo \$(seqkit --version 2>&1) | sed 's/^.*seqkit //; s/Using.*\$//' > ${software}.version.txt
"""
} else {
"""
seqkit \
split2 \
$options.args \
--threads $task.cpus \
-1 $read1 \
-2 $read2 \
-O $prefix
echo \$(seqkit --version 2>&1) | sed 's/^.*seqkit //; s/Using.*\$//' > ${software}.version.txt
"""
}
}