2020-09-10 15:45:11 +00:00
|
|
|
process MACS2_CALLPEAK {
|
|
|
|
tag "$meta.id"
|
|
|
|
label 'process_medium'
|
|
|
|
|
2021-02-16 23:58:23 +00:00
|
|
|
conda (params.enable_conda ? "bioconda::macs2=2.2.7.1" : null)
|
2021-11-26 07:58:40 +00:00
|
|
|
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
|
|
|
'https://depot.galaxyproject.org/singularity/macs2:2.2.7.1--py38h4a8c8d9_3' :
|
|
|
|
'quay.io/biocontainers/macs2:2.2.7.1--py38h4a8c8d9_3' }"
|
2020-09-10 15:45:11 +00:00
|
|
|
|
|
|
|
input:
|
|
|
|
tuple val(meta), path(ipbam), path(controlbam)
|
|
|
|
val macs2_gsize
|
2020-12-17 23:50:24 +00:00
|
|
|
|
2020-09-10 15:45:11 +00:00
|
|
|
output:
|
|
|
|
tuple val(meta), path("*.{narrowPeak,broadPeak}"), emit: peak
|
|
|
|
tuple val(meta), path("*.xls") , emit: xls
|
2021-10-01 13:04:56 +00:00
|
|
|
path "versions.yml" , emit: versions
|
2020-09-10 15:45:11 +00:00
|
|
|
|
|
|
|
tuple val(meta), path("*.gappedPeak"), optional:true, emit: gapped
|
|
|
|
tuple val(meta), path("*.bed") , optional:true, emit: bed
|
|
|
|
tuple val(meta), path("*.bdg") , optional:true, emit: bdg
|
|
|
|
|
2022-02-04 08:53:32 +00:00
|
|
|
when:
|
|
|
|
task.ext.when == null || task.ext.when
|
|
|
|
|
2020-09-10 15:45:11 +00:00
|
|
|
script:
|
2021-11-26 07:58:40 +00:00
|
|
|
def args = task.ext.args ?: ''
|
2021-12-02 12:39:55 +00:00
|
|
|
def prefix = task.ext.prefix ?: "${meta.id}"
|
2021-11-26 07:58:40 +00:00
|
|
|
def args_list = args.tokenize()
|
|
|
|
def format = meta.single_end ? 'BAM' : 'BAMPE'
|
|
|
|
def control = controlbam ? "--control $controlbam" : ''
|
|
|
|
if(args_list.contains('--format')){
|
|
|
|
def id = args_list.findIndexOf{it=='--format'}
|
|
|
|
format = args_list[id+1]
|
|
|
|
args_list.remove(id+1)
|
|
|
|
args_list.remove(id)
|
2021-11-15 17:17:40 +00:00
|
|
|
}
|
2020-09-10 15:45:11 +00:00
|
|
|
"""
|
|
|
|
macs2 \\
|
|
|
|
callpeak \\
|
2021-11-26 07:58:40 +00:00
|
|
|
${args_list.join(' ')} \\
|
2020-09-10 15:45:11 +00:00
|
|
|
--gsize $macs2_gsize \\
|
|
|
|
--format $format \\
|
|
|
|
--name $prefix \\
|
|
|
|
--treatment $ipbam \\
|
2020-12-17 23:50:24 +00:00
|
|
|
$control
|
2020-09-10 15:45:11 +00:00
|
|
|
|
2021-09-27 08:41:24 +00:00
|
|
|
cat <<-END_VERSIONS > versions.yml
|
2021-11-26 07:58:40 +00:00
|
|
|
"${task.process}":
|
|
|
|
macs2: \$(macs2 --version | sed -e "s/macs2 //g")
|
2021-09-27 08:41:24 +00:00
|
|
|
END_VERSIONS
|
2020-09-10 15:45:11 +00:00
|
|
|
"""
|
|
|
|
}
|