2021-02-12 23:08:57 +00:00
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process BCFTOOLS_MERGE {
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tag "$meta.id"
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2021-02-24 08:47:54 +00:00
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label 'process_medium'
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2021-02-12 23:08:57 +00:00
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2022-07-07 13:48:04 +00:00
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conda (params.enable_conda ? "bioconda::bcftools=1.15.1" : null)
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2021-11-26 07:58:40 +00:00
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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2022-07-07 13:48:04 +00:00
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'https://depot.galaxyproject.org/singularity/bcftools:1.15.1--h0ea216a_0':
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'quay.io/biocontainers/bcftools:1.15.1--h0ea216a_0' }"
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2021-02-12 23:08:57 +00:00
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input:
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tuple val(meta), path(vcfs), path(tbis)
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2022-07-20 09:22:51 +00:00
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path bed
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path fasta
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path fasta_fai
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2021-02-12 23:08:57 +00:00
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output:
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2022-07-20 09:22:51 +00:00
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tuple val(meta), path("*.{bcf,vcf}{,.gz}"), emit: merged_variants
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path "versions.yml" , emit: versions
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2021-02-12 23:08:57 +00:00
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2022-02-04 08:53:32 +00:00
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when:
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task.ext.when == null || task.ext.when
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2021-02-12 23:08:57 +00:00
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script:
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2021-12-07 14:00:43 +00:00
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def args = task.ext.args ?: ''
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2022-07-20 09:22:51 +00:00
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def prefix = task.ext.prefix ?: "${meta.id}"
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def regions = bed ? "--regions-file $bed" : ""
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def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" :
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args.contains("--output-type u") || args.contains("-Ou") ? "bcf" :
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args.contains("--output-type v") || args.contains("-Ov") ? "vcf" :
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"vcf.gz"
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2021-02-12 23:08:57 +00:00
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"""
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2022-07-20 09:22:51 +00:00
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bcftools merge \\
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$regions \\
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--threads $task.cpus \\
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--output ${prefix}.${extension} \\
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2021-11-26 07:58:40 +00:00
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$args \\
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2021-02-12 23:08:57 +00:00
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*.vcf.gz
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2022-07-20 09:22:51 +00:00
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2021-09-27 08:41:24 +00:00
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cat <<-END_VERSIONS > versions.yml
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2021-11-26 07:58:40 +00:00
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"${task.process}":
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bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//')
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2021-09-27 08:41:24 +00:00
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END_VERSIONS
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2021-02-12 23:08:57 +00:00
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"""
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}
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