2021-02-03 16:02:58 +00:00
|
|
|
#!/usr/bin/env nextflow
|
|
|
|
|
|
|
|
nextflow.enable.dsl = 2
|
|
|
|
|
2021-11-26 07:58:40 +00:00
|
|
|
include { BOWTIE2_BUILD } from '../../../../modules/bowtie2/build/main.nf'
|
|
|
|
include { BOWTIE2_ALIGN } from '../../../../modules/bowtie2/align/main.nf'
|
2021-02-03 16:02:58 +00:00
|
|
|
|
|
|
|
workflow test_bowtie2_align_single_end {
|
2021-11-26 07:58:40 +00:00
|
|
|
input = [
|
|
|
|
[ id:'test', single_end:true ], // meta map
|
|
|
|
[
|
|
|
|
file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
|
|
|
|
]
|
|
|
|
]
|
2021-03-24 18:29:41 +00:00
|
|
|
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
2022-01-07 17:52:39 +00:00
|
|
|
save_unaligned = false
|
2022-05-13 13:26:41 +00:00
|
|
|
sort = false
|
2021-02-03 16:02:58 +00:00
|
|
|
|
|
|
|
BOWTIE2_BUILD ( fasta )
|
2022-05-13 13:26:41 +00:00
|
|
|
BOWTIE2_ALIGN ( input, BOWTIE2_BUILD.out.index, save_unaligned, sort )
|
|
|
|
}
|
|
|
|
|
|
|
|
workflow test_bowtie2_align_single_end_sorted {
|
|
|
|
input = [
|
|
|
|
[ id:'test', single_end:true ], // meta map
|
|
|
|
[
|
|
|
|
file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
|
|
|
|
]
|
|
|
|
]
|
|
|
|
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
|
|
|
save_unaligned = false
|
|
|
|
sort = true
|
|
|
|
|
|
|
|
BOWTIE2_BUILD ( fasta )
|
|
|
|
BOWTIE2_ALIGN ( input, BOWTIE2_BUILD.out.index, save_unaligned, sort )
|
2021-02-03 16:02:58 +00:00
|
|
|
}
|
|
|
|
|
|
|
|
workflow test_bowtie2_align_paired_end {
|
2021-11-26 07:58:40 +00:00
|
|
|
input = [
|
|
|
|
[ id:'test', single_end:false ], // meta map
|
|
|
|
[
|
|
|
|
file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
|
|
|
|
file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
|
|
|
|
]
|
|
|
|
]
|
2021-03-24 18:29:41 +00:00
|
|
|
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
2022-01-07 17:52:39 +00:00
|
|
|
save_unaligned = false
|
2022-05-13 13:26:41 +00:00
|
|
|
sort = false
|
2021-11-26 07:58:40 +00:00
|
|
|
|
2022-05-12 09:01:33 +00:00
|
|
|
BOWTIE2_BUILD ( fasta )
|
2022-05-13 13:26:41 +00:00
|
|
|
BOWTIE2_ALIGN ( input, BOWTIE2_BUILD.out.index, save_unaligned, sort )
|
2022-05-12 09:01:33 +00:00
|
|
|
}
|
|
|
|
|
|
|
|
workflow test_bowtie2_align_single_end_large_index {
|
|
|
|
input = [
|
|
|
|
[ id:'test', single_end:true ], // meta map
|
|
|
|
[
|
|
|
|
file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)
|
|
|
|
]
|
|
|
|
]
|
|
|
|
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
|
|
|
save_unaligned = false
|
2022-05-13 13:26:41 +00:00
|
|
|
sort = false
|
2022-05-12 09:01:33 +00:00
|
|
|
|
|
|
|
BOWTIE2_BUILD ( fasta )
|
2022-05-13 13:26:41 +00:00
|
|
|
BOWTIE2_ALIGN ( input, BOWTIE2_BUILD.out.index, save_unaligned, sort )
|
2022-05-12 09:01:33 +00:00
|
|
|
}
|
|
|
|
|
|
|
|
workflow test_bowtie2_align_paired_end_large_index {
|
|
|
|
input = [
|
|
|
|
[ id:'test', single_end:false ], // meta map
|
|
|
|
[
|
|
|
|
file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
|
|
|
|
file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)
|
|
|
|
]
|
|
|
|
]
|
|
|
|
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
|
|
|
save_unaligned = false
|
2022-05-13 13:26:41 +00:00
|
|
|
sort = false
|
2022-05-12 09:01:33 +00:00
|
|
|
|
2021-02-03 16:02:58 +00:00
|
|
|
BOWTIE2_BUILD ( fasta )
|
2022-05-13 13:26:41 +00:00
|
|
|
BOWTIE2_ALIGN ( input, BOWTIE2_BUILD.out.index, save_unaligned, sort )
|
|
|
|
}
|