nf-core_modules/software/vcftools/main.nf

// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'

params.options = [:]
options        = initOptions(params.options)

process VCFTOOLS {
    tag "$meta.id"
    label 'process_medium'
    publishDir "${params.outdir}",
        mode: params.publish_dir_mode,
        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }

    conda (params.enable_conda ? "bioconda::vcftools=0.1.16" : null)
    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
        container "https://depot.galaxyproject.org/singularity/vcftools:0.1.16--he513fc3_4"
    } else {
        container "quay.io/biocontainers/vcftools:0.1.16--he513fc3_4"
    }

    input:
    // Owing to the nature of vcftools we here provide solutions to working with optional bed files and optional
    // alternative variant files, for use with the 'diff' suite of tools.
    // Other optional input files can be utilised in a similar way to below but we do not exhaustively itterate through all
    // possible options. Instead we leave that to the user.
    tuple val(meta), path(variant_file)
    path(bed)
    path(diff_variant_file)

    output:
    path("*.version.txt"), emit: version

    tuple val(meta), path("*.vcf"), optional:true, emit: vcf
    tuple val(meta), path("*.bcf"), optional:true, emit: bcf
    tuple val(meta), path("*.frq"), optional:true, emit: frq
    tuple val(meta), path("*.frq.count"), optional:true, emit: frq_count
    tuple val(meta), path("*.idepth"), optional:true, emit: idepth
    tuple val(meta), path("*.ldepth"), optional:true, emit: ldepth
    tuple val(meta), path("*.ldepth.mean"), optional:true, emit: ldepth_mean
    tuple val(meta), path("*.gdepth"), optional:true, emit: gdepth
    tuple val(meta), path("*.hap.ld"), optional:true, emit: hap_ld
    tuple val(meta), path("*.geno.ld"), optional:true, emit: geno_ld
    tuple val(meta), path("*.geno.chisq"), optional:true, emit: geno_chisq
    tuple val(meta), path("*.list.hap.ld"), optional:true, emit: list_hap_ld
    tuple val(meta), path("*.list.geno.ld"), optional:true, emit: list_geno_ld
    tuple val(meta), path("*.interchrom.hap.ld"), optional:true, emit: interchrom_hap_ld
    tuple val(meta), path("*.interchrom.geno.ld"), optional:true, emit: interchrom_geno_ld
    tuple val(meta), path("*.TsTv"), optional:true, emit: tstv
    tuple val(meta), path("*.TsTv.summary"), optional:true, emit: tstv_summary
    tuple val(meta), path("*.TsTv.count"), optional:true, emit: tstv_count
    tuple val(meta), path("*.TsTv.qual"), optional:true, emit: tstv_qual
    tuple val(meta), path("*.FILTER.summary"), optional:true, emit: filter_summary
    tuple val(meta), path("*.sites.pi"), optional:true, emit: sites_pi
    tuple val(meta), path("*.windowed.pi"), optional:true, emit: windowed_pi
    tuple val(meta), path("*.weir.fst"), optional:true, emit: weir_fst
    tuple val(meta), path("*.het"), optional:true, emit: heterozygosity
    tuple val(meta), path("*.hwe"), optional:true,  emit: hwe
    tuple val(meta), path("*.Tajima.D"), optional:true, emit: tajima_d
    tuple val(meta), path("*.ifreqburden"), optional:true, emit: freq_burden
    tuple val(meta), path("*.LROH"), optional:true, emit: lroh
    tuple val(meta), path("*.relatedness"), optional:true, emit: relatedness
    tuple val(meta), path("*.relatedness2"), optional:true, emit: relatedness2
    tuple val(meta), path("*.lqual"), optional:true, emit: lqual
    tuple val(meta), path("*.imiss"), optional:true, emit: missing_individual
    tuple val(meta), path("*.lmiss"), optional:true, emit: missing_site
    tuple val(meta), path("*.snpden"), optional:true, emit: snp_density
    tuple val(meta), path("*.kept.sites"), optional:true, emit: kept_sites
    tuple val(meta), path("*.removed.sites"), optional:true, emit: removed_sites
    tuple val(meta), path("*.singletons"), optional:true, emit: singeltons
    tuple val(meta), path("*.indel.hist"), optional:true, emit: indel_hist
    tuple val(meta), path("*.hapcount"), optional:true, emit: hapcount
    tuple val(meta), path("*.mendel"), optional:true, emit: mendel
    tuple val(meta), path("*.FORMAT"), optional:true, emit: format
    tuple val(meta), path("*.INFO"), optional:true, emit: info
    tuple val(meta), path("*.012"), optional:true, emit: genotypes_matrix
    tuple val(meta), path("*.012.indv"), optional:true, emit: genotypes_matrix_individual
    tuple val(meta), path("*.012.pos"), optional:true, emit: genotypes_matrix_position
    tuple val(meta), path("*.impute.hap"), optional:true, emit: impute_hap
    tuple val(meta), path("*.impute.hap.legend"), optional:true, emit: impute_hap_legend
    tuple val(meta), path("*.impute.hap.indv"), optional:true, emit: impute_hap_indv
    tuple val(meta), path("*.ldhat.sites"), optional:true, emit: ldhat_sites
    tuple val(meta), path("*.ldhat.locs"), optional:true, emit: ldhat_locs
    tuple val(meta), path("*.BEAGLE.GL"), optional:true, emit: beagle_gl
    tuple val(meta), path("*.BEAGLE.PL"), optional:true, emit: beagle_pl
    tuple val(meta), path("*.ped"), optional:true, emit: ped
    tuple val(meta), path("*.map"), optional:true, emit: map_
    tuple val(meta), path("*.tped"), optional:true, emit: tped
    tuple val(meta), path("*.tfam"), optional:true, emit: tfam
    tuple val(meta), path("*.diff.sites_in_files"), optional:true, emit: diff_sites_in_files
    tuple val(meta), path("*.diff.indv_in_files"), optional:true, emit: diff_indv_in_files
    tuple val(meta), path("*.diff.sites"), optional:true, emit: diff_sites
    tuple val(meta), path("*.diff.indv"), optional:true, emit: diff_indv
    tuple val(meta), path("*.diff.discordance.matrix"), optional:true, emit: diff_discd_matrix
    tuple val(meta), path("*.diff.switch"), optional:true, emit: diff_switch_error

    script:
    def software = getSoftwareName(task.process)
    def prefix   = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
    def args     = options.args.tokenize()

    def bed_arg  = (options.args.contains('--bed')) ? "--bed ${bed}" :
        (options.args.contains('--exclude-bed')) ? "--exclude-bed ${bed}" :
        (options.args.contains('--hapcount')) ? "--hapcount ${bed}" : ''
    args.removeIf { it.contains('--bed') }
    args.removeIf { it.contains('--exclude-bed') }
    args.removeIf { it.contains('--hapcount') }

    def diff_variant_arg = (options.args.contains('--diff')) ? "--diff ${diff_variant_file}" :
        (options.args.contains('--gzdiff')) ? "--gzdiff ${diff_variant_file}" :
        (options.args.contains('--diff-bcf')) ? "--diff-bcf ${diff_variant_file}" : ''
    args.removeIf { it.contains('--diff') }
    args.removeIf { it.contains('--gzdiff') }
    args.removeIf { it.contains('--diff-bcf') }

    def input_file = ("$variant_file".endsWith(".vcf")) ? "--vcf ${variant_file}" :
        ("$variant_file".endsWith(".vcf.gz")) ? "--gzvcf ${variant_file}" :
        ("$variant_file".endsWith(".bcf")) ? "--bcf ${variant_file}" : ''

    """
    vcftools \\
        $input_file \\
        --out $prefix \\
        ${args.join(' ')} \\
        $bed_arg \\
        $diff_variant_arg \\

    echo \$(vcftools --version 2>&1) | sed 's/^.*vcftools //; s/Using.*\$//' > ${software}.version.txt
    """
}