2021-05-10 06:45:52 -04:00
|
|
|
#!/usr/bin/env nextflow
|
|
|
|
|
|
|
|
nextflow.enable.dsl = 2
|
|
|
|
|
2021-07-07 05:10:18 -04:00
|
|
|
include { BBMAP_BBDUK } from '../../../../modules/bbmap/bbduk/main.nf' addParams( options: [ 'args' : 'trimq=10 qtrim=r', 'suffix' : '.trim' ] )
|
2021-05-10 06:45:52 -04:00
|
|
|
|
|
|
|
workflow test_bbmap_bbduk_single_end {
|
|
|
|
|
|
|
|
input = [ [ id:'test', single_end:true ], // meta map
|
|
|
|
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
|
|
|
|
]
|
|
|
|
|
|
|
|
BBMAP_BBDUK ( input, [] )
|
|
|
|
}
|
|
|
|
|
|
|
|
workflow test_bbmap_bbduk_paired_end {
|
|
|
|
|
|
|
|
input = [ [ id:'test', single_end:false ], // meta map
|
|
|
|
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
|
|
|
|
file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
|
|
|
|
]
|
|
|
|
|
|
|
|
BBMAP_BBDUK ( input, [] )
|
|
|
|
}
|
|
|
|
|
|
|
|
workflow test_bbmap_bbduk_se_ref {
|
|
|
|
|
|
|
|
input = [ [ id:'test', single_end:true ], // meta map
|
|
|
|
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
|
|
|
|
]
|
|
|
|
contaminants = [file(params.test_data['sarscov2']['genome']['transcriptome_fasta'], checkIfExists: true) ] // transciptome file - remove contaminants (*trim.fastq files empty)
|
|
|
|
|
|
|
|
BBMAP_BBDUK ( input, contaminants )
|
|
|
|
}
|
|
|
|
|
|
|
|
workflow test_bbmap_bbduk_pe_ref {
|
|
|
|
|
|
|
|
input = [ [ id:'test', single_end:false ], // meta map
|
|
|
|
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
|
|
|
|
file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
|
|
|
|
]
|
|
|
|
contaminants = [file(params.test_data['sarscov2']['genome']['transcriptome_fasta'], checkIfExists: true) ]
|
|
|
|
|
|
|
|
BBMAP_BBDUK ( input, contaminants )
|
|
|
|
}
|