2021-03-20 23:54:42 +00:00
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// Import generic module functions
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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params.options = [:]
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options = initOptions(params.options)
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2021-03-22 16:46:11 +00:00
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process BISMARK_METHYLATIONEXTRACTOR {
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2021-03-20 23:54:42 +00:00
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tag "$meta.id"
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label 'process_high'
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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2021-04-09 16:23:56 +00:00
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saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
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2021-03-20 23:54:42 +00:00
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conda (params.enable_conda ? "bioconda::bismark=0.23.0" : null)
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if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
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container "https://depot.galaxyproject.org/singularity/bismark:0.23.0--0"
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} else {
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container "quay.io/biocontainers/bismark:0.23.0--0"
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}
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input:
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tuple val(meta), path(bam)
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path index
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output:
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tuple val(meta), path("*.bedGraph.gz") , emit: bedgraph
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tuple val(meta), path("*.txt.gz") , emit: methylation_calls
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tuple val(meta), path("*.cov.gz") , emit: coverage
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tuple val(meta), path("*_splitting_report.txt"), emit: report
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tuple val(meta), path("*.M-bias.txt") , emit: mbias
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path "*.version.txt" , emit: version
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script:
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def seqtype = meta.single_end ? '-s' : '-p'
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def software = getSoftwareName(task.process)
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"""
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bismark_methylation_extractor \\
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--bedGraph \\
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--counts \\
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--gzip \\
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--report \\
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$seqtype \\
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$options.args \\
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$bam
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echo \$(bismark -v 2>&1) | sed 's/^.*Bismark Version: v//; s/Copyright.*\$//' > ${software}.version.txt
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"""
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}
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