2020-12-17 23:50:24 +00:00
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// Import generic module functions
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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params.options = [:]
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2021-03-15 12:16:43 +00:00
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options = initOptions(params.options)
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2020-12-17 23:50:24 +00:00
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process UMITOOLS_DEDUP {
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tag "$meta.id"
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label "process_medium"
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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2021-04-09 16:23:56 +00:00
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saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
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2020-12-17 23:50:24 +00:00
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2021-07-16 13:21:08 +00:00
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conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
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2020-12-17 23:50:24 +00:00
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if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
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2021-07-16 13:21:08 +00:00
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container "https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0"
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2020-12-17 23:50:24 +00:00
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} else {
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2021-07-16 13:21:08 +00:00
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container "quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0"
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2020-12-17 23:50:24 +00:00
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}
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input:
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tuple val(meta), path(bam), path(bai)
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output:
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tuple val(meta), path("*.bam"), emit: bam
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path "*.version.txt" , emit: version
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script:
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def software = getSoftwareName(task.process)
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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2021-07-16 13:21:08 +00:00
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def paired = meta.single_end ? "" : "--paired"
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2020-12-17 23:50:24 +00:00
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"""
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umi_tools dedup \\
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-I $bam \\
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-S ${prefix}.bam \\
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2021-07-16 13:21:08 +00:00
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$paired \\
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$options.args
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2020-12-17 23:50:24 +00:00
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2021-07-16 13:21:08 +00:00
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echo \$(umi_tools --version 2>&1) | sed 's/^.*UMI-tools version://; s/ *\$//' > ${software}.version.txt
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2020-12-17 23:50:24 +00:00
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"""
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}
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