2021-02-12 12:00:02 +00:00
|
|
|
// Import generic module functions
|
2021-09-27 08:41:24 +00:00
|
|
|
include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions'
|
2021-02-12 12:00:02 +00:00
|
|
|
|
|
|
|
params.options = [:]
|
2021-03-15 12:16:43 +00:00
|
|
|
options = initOptions(params.options)
|
2021-02-12 12:00:02 +00:00
|
|
|
|
|
|
|
process BWAMEM2_MEM {
|
|
|
|
tag "$meta.id"
|
|
|
|
label 'process_high'
|
|
|
|
publishDir "${params.outdir}",
|
|
|
|
mode: params.publish_dir_mode,
|
2021-04-09 16:23:56 +00:00
|
|
|
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
|
2021-02-12 12:00:02 +00:00
|
|
|
|
2021-03-25 09:24:21 +00:00
|
|
|
conda (params.enable_conda ? "bioconda::bwa-mem2=2.2.1 bioconda::samtools=1.12" : null)
|
2021-02-12 12:00:02 +00:00
|
|
|
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
|
2021-03-25 09:24:21 +00:00
|
|
|
container "https://depot.galaxyproject.org/singularity/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:cf603b12db30ec91daa04ba45a8ee0f35bbcd1e2-0"
|
2021-02-12 12:00:02 +00:00
|
|
|
} else {
|
2021-03-25 09:24:21 +00:00
|
|
|
container "quay.io/biocontainers/mulled-v2-e5d375990341c5aef3c9aff74f96f66f65375ef6:cf603b12db30ec91daa04ba45a8ee0f35bbcd1e2-0"
|
2021-02-12 12:00:02 +00:00
|
|
|
}
|
|
|
|
|
|
|
|
input:
|
|
|
|
tuple val(meta), path(reads)
|
|
|
|
path index
|
|
|
|
|
|
|
|
output:
|
|
|
|
tuple val(meta), path("*.bam"), emit: bam
|
2021-09-27 08:41:24 +00:00
|
|
|
path "versions.yml" , emit: version
|
2021-02-12 12:00:02 +00:00
|
|
|
|
|
|
|
script:
|
|
|
|
def software = getSoftwareName(task.process)
|
|
|
|
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
|
|
|
|
def read_group = meta.read_group ? "-R ${meta.read_group}" : ""
|
|
|
|
"""
|
|
|
|
INDEX=`find -L ./ -name "*.amb" | sed 's/.amb//'`
|
|
|
|
|
2021-09-27 21:10:37 +00:00
|
|
|
bwa-mem2 \\
|
|
|
|
mem \\
|
2021-02-12 12:00:02 +00:00
|
|
|
$options.args \\
|
|
|
|
$read_group \\
|
2021-09-30 08:26:52 +00:00
|
|
|
-t $task.cpus \\
|
2021-02-12 12:00:02 +00:00
|
|
|
\$INDEX \\
|
|
|
|
$reads \\
|
2021-09-30 08:26:52 +00:00
|
|
|
| samtools view $options.args2 -@ $task.cpus -bhS -o ${prefix}.bam -
|
2021-02-12 12:00:02 +00:00
|
|
|
|
2021-09-27 08:41:24 +00:00
|
|
|
cat <<-END_VERSIONS > versions.yml
|
|
|
|
${getProcessName(task.process)}:
|
2021-09-27 21:10:37 +00:00
|
|
|
${getSoftwareName(task.process)}: \$(echo \$(bwa-mem2 version 2>&1) | sed 's/.* //')
|
2021-09-28 13:37:47 +00:00
|
|
|
samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
|
2021-09-27 08:41:24 +00:00
|
|
|
END_VERSIONS
|
2021-02-12 12:00:02 +00:00
|
|
|
"""
|
|
|
|
}
|