2020-09-10 15:45:11 +00:00
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process BWA_MEM {
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tag "$meta.id"
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label 'process_high'
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2022-04-11 12:26:28 +00:00
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conda (params.enable_conda ? "bioconda::bwa=0.7.17 bioconda::samtools=1.15.1" : null)
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2021-11-26 07:58:40 +00:00
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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2022-04-11 12:26:28 +00:00
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'https://depot.galaxyproject.org/singularity/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:8110a70be2bfe7f75a2ea7f2a89cda4cc7732095-0' :
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'quay.io/biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:8110a70be2bfe7f75a2ea7f2a89cda4cc7732095-0' }"
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2020-12-17 23:50:24 +00:00
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2020-09-10 15:45:11 +00:00
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input:
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tuple val(meta), path(reads)
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path index
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2021-12-08 14:31:27 +00:00
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val sort_bam
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2020-12-17 23:50:24 +00:00
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2020-09-10 15:45:11 +00:00
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output:
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tuple val(meta), path("*.bam"), emit: bam
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2021-10-01 13:04:56 +00:00
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path "versions.yml" , emit: versions
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2020-09-10 15:45:11 +00:00
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2022-02-04 08:53:32 +00:00
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when:
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task.ext.when == null || task.ext.when
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2020-09-10 15:45:11 +00:00
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script:
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2021-11-26 07:58:40 +00:00
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def args = task.ext.args ?: ''
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def args2 = task.ext.args2 ?: ''
|
2021-12-02 12:39:55 +00:00
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def prefix = task.ext.prefix ?: "${meta.id}"
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2021-12-08 14:31:27 +00:00
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def samtools_command = sort_bam ? 'sort' : 'view'
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2020-09-10 15:45:11 +00:00
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"""
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2021-02-02 12:34:42 +00:00
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INDEX=`find -L ./ -name "*.amb" | sed 's/.amb//'`
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2020-09-10 15:45:11 +00:00
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bwa mem \\
|
2021-11-26 07:58:40 +00:00
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$args \\
|
2021-09-30 08:26:52 +00:00
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-t $task.cpus \\
|
2021-02-02 12:34:42 +00:00
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\$INDEX \\
|
2020-09-10 15:45:11 +00:00
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$reads \\
|
2021-12-08 14:31:27 +00:00
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| samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.bam -
|
2020-09-10 15:45:11 +00:00
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|
2021-09-27 08:41:24 +00:00
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|
cat <<-END_VERSIONS > versions.yml
|
2021-11-26 07:58:40 +00:00
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"${task.process}":
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bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//')
|
2021-09-28 13:37:47 +00:00
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samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
|
2021-09-27 08:41:24 +00:00
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END_VERSIONS
|
2020-09-10 15:45:11 +00:00
|
|
|
"""
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|
}
|