nf-core_modules/software/seqtk/sample/main.nf

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// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'
params.options = [:]
options = initOptions(params.options)
process SEQTK_SAMPLE {
tag "$meta.id"
label 'process_low'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
conda (params.enable_conda ? "bioconda::seqtk=1.3" : null)
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
container "https://depot.galaxyproject.org/singularity/seqtk:1.3--h5bf99c6_3"
} else {
container "quay.io/biocontainers/seqtk:1.3--h5bf99c6_3"
}
input:
tuple val(meta), path(reads)
val sample_size
output:
tuple val(meta), path("*.fastq.gz"), emit: reads
path "*.version.txt" , emit: version
script:
def software = getSoftwareName(task.process)
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
if (meta.single_end) {
"""
seqtk \\
sample \\
$options.args \\
$reads \\
$sample_size \\
| gzip > ${prefix}.fastq.gz \\
echo \$(seqtk 2>&1) | sed 's/^.*Version: //; s/ .*\$//' > ${software}.version.txt
"""
} else {
"""
seqtk \\
sample \\
$options.args \\
$reads[0] \\
$sample_size \\
| gzip > ${prefix}_1.fastq.gz \\
seqtk \\
sample \\
$options.args \\
$reads[1] \\
$sample_size \\
| gzip > ${prefix}_2.fastq.gz \\
echo \$(seqtk 2>&1) | sed 's/^.*Version: //; s/ .*\$//' > ${software}.version.txt
"""
}
}