2021-01-29 08:33:45 +00:00
|
|
|
// Import generic module functions
|
|
|
|
include { initOptions; saveFiles; getSoftwareName } from './functions'
|
|
|
|
|
|
|
|
params.options = [:]
|
2021-03-15 12:16:43 +00:00
|
|
|
options = initOptions(params.options)
|
2021-01-29 08:33:45 +00:00
|
|
|
|
|
|
|
process CUTADAPT {
|
|
|
|
tag "$meta.id"
|
|
|
|
label 'process_medium'
|
|
|
|
publishDir "${params.outdir}",
|
|
|
|
mode: params.publish_dir_mode,
|
2021-04-09 16:23:56 +00:00
|
|
|
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
|
2021-01-29 08:33:45 +00:00
|
|
|
|
2021-02-24 14:52:25 +00:00
|
|
|
conda (params.enable_conda ? 'bioconda::cutadapt=3.2' : null)
|
2021-01-29 08:33:45 +00:00
|
|
|
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
|
|
|
|
container 'https://depot.galaxyproject.org/singularity/cutadapt:3.2--py38h0213d0e_0'
|
|
|
|
} else {
|
|
|
|
container 'quay.io/biocontainers/cutadapt:3.2--py38h0213d0e_0'
|
|
|
|
}
|
|
|
|
|
|
|
|
input:
|
|
|
|
tuple val(meta), path(reads)
|
|
|
|
|
|
|
|
output:
|
|
|
|
tuple val(meta), path('*.trim.fastq.gz'), emit: reads
|
|
|
|
tuple val(meta), path('*.log') , emit: log
|
|
|
|
path '*.version.txt' , emit: version
|
|
|
|
|
|
|
|
script:
|
|
|
|
def software = getSoftwareName(task.process)
|
|
|
|
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
|
|
|
|
def trimmed = meta.single_end ? "-o ${prefix}.trim.fastq.gz" : "-o ${prefix}_1.trim.fastq.gz -p ${prefix}_2.trim.fastq.gz"
|
|
|
|
"""
|
|
|
|
cutadapt \\
|
|
|
|
--cores $task.cpus \\
|
|
|
|
$options.args \\
|
|
|
|
$trimmed \\
|
|
|
|
$reads \\
|
|
|
|
> ${prefix}.cutadapt.log
|
|
|
|
echo \$(cutadapt --version) > ${software}.version.txt
|
|
|
|
"""
|
|
|
|
}
|