nf-core_modules/modules/rsem/preparereference/main.nf

// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'

params.options = [:]
options        = initOptions(params.options)

process RSEM_PREPAREREFERENCE {
    tag "$fasta"
    label 'process_high'
    publishDir "${params.outdir}",
        mode: params.publish_dir_mode,
        saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:'index', meta:[:], publish_by_meta:[]) }

    conda (params.enable_conda ? "bioconda::rsem=1.3.3 bioconda::star=2.7.6a" : null)
    if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
        container "https://depot.galaxyproject.org/singularity/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:606b713ec440e799d53a2b51a6e79dbfd28ecf3e-0"
    } else {
        container "quay.io/biocontainers/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:606b713ec440e799d53a2b51a6e79dbfd28ecf3e-0"
    }

    input:
    path fasta, stageAs: "rsem/*"
    path gtf

    output:
    path "rsem"                , emit: index
    path "rsem/*transcripts.fa", emit: transcript_fasta
    path "*.version.txt"       , emit: version

    script:
    def software = getSoftwareName(task.process)
    def args     = options.args.tokenize()
    if (args.contains('--star')) {
        args.removeIf { it.contains('--star') }
        def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : ''
        """
        STAR \\
            --runMode genomeGenerate \\
            --genomeDir rsem/ \\
            --genomeFastaFiles $fasta \\
            --sjdbGTFfile $gtf \\
            --runThreadN $task.cpus \\
            $memory \\
            $options.args2

        rsem-prepare-reference \\
            --gtf $gtf \\
            --num-threads $task.cpus \\
            ${args.join(' ')} \\
            $fasta \\
            rsem/genome

        rsem-calculate-expression --version | sed -e "s/Current version: RSEM v//g" > ${software}.version.txt
        """
    } else {
        """
        rsem-prepare-reference \\
            --gtf $gtf \\
            --num-threads $task.cpus \\
            $options.args \\
            $fasta \\
            rsem/genome

        rsem-calculate-expression --version | sed -e "s/Current version: RSEM v//g" > ${software}.version.txt
        """
    }
}