description:tool that takes either fragmented metagenomic data or longer sequences as input and predicts and delivers full-length antiobiotic resistance genes as output.
keywords:
- antibiotic resistance genes
- ARGs
- identifier
- metagenomic
- contigs
tools:
- fargene:
description:Fragmented Antibiotic Resistance Gene Identifier takes either fragmented metagenomic data or longer sequences as input and predicts and delivers full-length antiobiotic resistance genes as output
description:uncompressed fasta file or paired-end fastq files containing either genomes or longer contigs as nucleotide or protein sequences (fasta) or fragmented metagenomic reads (fastq)
description:name of custom hidden markov model to be used [pre-defined class_a, class_b_1_2, class_b_3, class_c, class_d_1, class_d_2, qnr, tet_efflux, tet_rpg, tet_enzyme]
output:
- meta:
type:map
description:|
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- versions:
type:file
description:File containing software versions
pattern:"versions.yml"
- log:
type:file
description:log file
pattern:"*.{log}"
- txt:
type:file
description:analysis summary text file
pattern:"*.{txt}"
- hmm:
type:file
description:output from hmmsearch
pattern:"*.{out}"
- orfs:
type:file
description:open reading frames (ORFs)
pattern:"*.{fasta}"
- orfs_amino:
type:file
description:protein translation of open reading frames (ORFs)
pattern:"*.{fasta}"
- contigs:
type:file
description:(complete) contigs that passed the final full-length classification
pattern:"*.{fasta}"
- contigs_pept:
type:file
description:parts of the contigs that passed the final classification step that aligned with the HMM, as amino acid sequences
pattern:"*.{fasta}"
- filtered:
type:file
description:sequences that passed the final classification step, but only the parts that where predicted by the HMM to be part of the gene
pattern:"*.{fasta}"
- filtered_pept:
type:file
description:sequences from filtered.fasta, translated in the same frame as the gene is predicted to be located
pattern:"*.{fasta}"
- fragments:
type:file
description:All quality controlled retrieved fragments that were classified as positive, together with its read-pair, gathered in two files
pattern:"*.{fastq}"
- trimmed:
type:file
description:The quality controlled retrieved fragments from each input file.
pattern:"*.{fasta}"
- spades:
type:directory
description:The output from the SPAdes assembly
pattern:"spades_assembly"
- metagenome:
type:file
description:The FASTQ to FASTA converted input files from metagenomic reads.
pattern:"*.{fasta}"
- tmp:
type:file
description:The from FASTQ to FASTA converted input files and their translated input sequences. Are only saved if option --store-peptides is used.