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79 lines
2.5 KiB
YAML
79 lines
2.5 KiB
YAML
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name: biscuit_blaster
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description: A fast, compact one-liner to produce duplicate-marked, sorted, and indexed BAM files using Biscuit
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keywords:
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- biscuit
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- DNA methylation
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- WGBS
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- scWGBS
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- bisulfite sequencing
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- aligner
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- bam
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tools:
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- biscuit:
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description: A utility for analyzing sodium bisulfite conversion-based DNA methylation/modification data
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homepage: https://huishenlab.github.io/biscuit/
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documentation: https://huishenlab.github.io/biscuit/biscuitblaster/
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tool_dev_url: https://github.com/huishenlab/biscuit
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doi: ""
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licence: ["MIT"]
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- samblaster:
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description: |
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samblaster is a fast and flexible program for marking duplicates in read-id grouped paired-end SAM files.
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It can also optionally output discordant read pairs and/or split read mappings to separate SAM files,
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and/or unmapped/clipped reads to a separate FASTQ file.
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By default, samblaster reads SAM input from stdin and writes SAM to stdout.
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homepage: None
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documentation: https://github.com/GregoryFaust/samblaster
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tool_dev_url: https://github.com/GregoryFaust/samblaster
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doi: "10.1093/bioinformatics/btu314"
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licence: ["MIT"]
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- samtools:
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description: |
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SAMtools is a set of utilities for interacting with and post-processing
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short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
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These files are generated as output by short read aligners like BWA.
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homepage: http://www.htslib.org/
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documentation: hhttp://www.htslib.org/doc/samtools.html
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doi: 10.1093/bioinformatics/btp352
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licence: ["MIT"]
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input:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- reads:
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type: file
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description: |
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List of input fastq files of size 1 and 2 for single-end and paired-end data,
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respectively.
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- index:
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type: dir
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description: Biscuit genome index directory (generated with 'biscuit index')
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pattern: "BiscuitIndex"
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output:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- bam:
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type: file
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description: Output BAM file containing read alignments
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pattern: "*.{bam}"
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- bai:
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type: file
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description: Output BAM index
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pattern: "*.{bai}"
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- versions:
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type: file
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description: File containing software versions
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pattern: "versions.yml"
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authors:
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- "@njspix"
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