2020-09-10 15:45:11 +00:00
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// Import generic module functions
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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2020-10-14 17:59:25 +00:00
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params.options = [:]
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2021-03-15 12:16:43 +00:00
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options = initOptions(params.options)
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2020-10-14 17:59:25 +00:00
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2020-09-10 15:45:11 +00:00
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process MACS2_CALLPEAK {
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tag "$meta.id"
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label 'process_medium'
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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2021-04-09 16:23:56 +00:00
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saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
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2020-09-10 15:45:11 +00:00
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2021-02-16 23:58:23 +00:00
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conda (params.enable_conda ? "bioconda::macs2=2.2.7.1" : null)
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2020-12-14 00:05:48 +00:00
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if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
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container "https://depot.galaxyproject.org/singularity/macs2:2.2.7.1--py38h0213d0e_1"
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} else {
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container "quay.io/biocontainers/macs2:2.2.7.1--py38h0213d0e_1"
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}
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2020-09-10 15:45:11 +00:00
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input:
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tuple val(meta), path(ipbam), path(controlbam)
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val macs2_gsize
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2020-12-17 23:50:24 +00:00
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2020-09-10 15:45:11 +00:00
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output:
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tuple val(meta), path("*.{narrowPeak,broadPeak}"), emit: peak
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tuple val(meta), path("*.xls") , emit: xls
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path "*.version.txt" , emit: version
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tuple val(meta), path("*.gappedPeak"), optional:true, emit: gapped
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tuple val(meta), path("*.bed") , optional:true, emit: bed
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tuple val(meta), path("*.bdg") , optional:true, emit: bdg
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script:
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def software = getSoftwareName(task.process)
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2020-10-14 17:59:25 +00:00
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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2020-09-10 15:45:11 +00:00
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def format = meta.single_end ? 'BAM' : 'BAMPE'
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def control = controlbam ? "--control $controlbam" : ''
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"""
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macs2 \\
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callpeak \\
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2020-10-14 17:59:25 +00:00
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$options.args \\
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2020-09-10 15:45:11 +00:00
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--gsize $macs2_gsize \\
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--format $format \\
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--name $prefix \\
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--treatment $ipbam \\
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2020-12-17 23:50:24 +00:00
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$control
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2020-09-10 15:45:11 +00:00
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macs2 --version | sed -e "s/macs2 //g" > ${software}.version.txt
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"""
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}
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