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Merge branch 'new-module-hap.py' of github.com:nvnieuwk/modules into new-module-hap.py
This commit is contained in:
commit
000b8ee2e0
6 changed files with 63 additions and 8 deletions
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@ -29,6 +29,8 @@ process BOWTIE2_ALIGN {
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def unaligned = save_unaligned ? "--un-gz ${prefix}.unmapped.fastq.gz" : ''
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"""
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INDEX=`find -L ./ -name "*.rev.1.bt2" | sed 's/.rev.1.bt2//'`
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[ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed 's/.rev.1.bt2l//'`
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[ -z "\$INDEX" ] && echo "BT2 index files not found" 1>&2 && exit 1
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bowtie2 \\
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-x \$INDEX \\
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-U $reads \\
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@ -49,6 +51,8 @@ process BOWTIE2_ALIGN {
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def unaligned = save_unaligned ? "--un-conc-gz ${prefix}.unmapped.fastq.gz" : ''
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"""
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INDEX=`find -L ./ -name "*.rev.1.bt2" | sed 's/.rev.1.bt2//'`
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[ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed 's/.rev.1.bt2l//'`
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[ -z "\$INDEX" ] && echo "BT2 index files not found" 1>&2 && exit 1
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bowtie2 \\
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-x \$INDEX \\
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-1 ${reads[0]} \\
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@ -10,8 +10,7 @@ process VARDICTJAVA {
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'quay.io/biocontainers/vardict-java:1.8.3--hdfd78af_0' }"
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input:
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tuple val(meta), path(bam), path(bai)
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path(bed)
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tuple val(meta), path(bam), path(bai), path(bed)
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tuple path(fasta), path(fasta_fai)
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output:
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@ -32,4 +32,4 @@ workflow test_bowtie2_align_paired_end {
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BOWTIE2_BUILD ( fasta )
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BOWTIE2_ALIGN ( input, BOWTIE2_BUILD.out.index, save_unaligned )
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}
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}
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@ -1,5 +1,16 @@
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params {
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force_large_index = false
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}
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process {
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publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
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}
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if (params.force_large_index) {
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process {
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withName: BOWTIE2_BUILD {
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ext.args = '--large-index'
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}
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}
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}
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@ -39,3 +39,45 @@
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md5sum: 52be6950579598a990570fbcf5372184
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- path: ./output/bowtie2/bowtie2/genome.rev.2.bt2
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md5sum: e3b4ef343dea4dd571642010a7d09597
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- name: bowtie2 align single-end large-index
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command: nextflow run ./tests/modules/bowtie2/align -entry test_bowtie2_align_single_end -c ./tests/config/nextflow.config -c ./tests/modules/bowtie2/align/nextflow.config --force_large_index
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tags:
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- bowtie2
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- bowtie2/align
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files:
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- path: ./output/bowtie2/test.bam
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- path: ./output/bowtie2/test.bowtie2.log
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- path: ./output/bowtie2/bowtie2/genome.3.bt2l
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md5sum: 8952b3e0b1ce9a7a5916f2e147180853
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- path: ./output/bowtie2/bowtie2/genome.2.bt2l
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md5sum: 22c284084784a0720989595e0c9461fd
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- path: ./output/bowtie2/bowtie2/genome.1.bt2l
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md5sum: 07d811cd4e350d56267183d2ac7023a5
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- path: ./output/bowtie2/bowtie2/genome.4.bt2l
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md5sum: c25be5f8b0378abf7a58c8a880b87626
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- path: ./output/bowtie2/bowtie2/genome.rev.1.bt2l
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md5sum: fda48e35925fb24d1c0785f021981e25
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- path: ./output/bowtie2/bowtie2/genome.rev.2.bt2l
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md5sum: 802c26d32b970e1b105032b7ce7348b4
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- name: bowtie2 align paired-end large-index
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command: nextflow run ./tests/modules/bowtie2/align -entry test_bowtie2_align_paired_end -c ./tests/config/nextflow.config -c ./tests/modules/bowtie2/align/nextflow.config --force_large_index
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tags:
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- bowtie2
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- bowtie2/align
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files:
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- path: ./output/bowtie2/test.bam
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- path: ./output/bowtie2/test.bowtie2.log
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- path: ./output/bowtie2/bowtie2/genome.3.bt2l
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md5sum: 8952b3e0b1ce9a7a5916f2e147180853
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- path: ./output/bowtie2/bowtie2/genome.2.bt2l
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md5sum: 22c284084784a0720989595e0c9461fd
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- path: ./output/bowtie2/bowtie2/genome.1.bt2l
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md5sum: 07d811cd4e350d56267183d2ac7023a5
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- path: ./output/bowtie2/bowtie2/genome.4.bt2l
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md5sum: c25be5f8b0378abf7a58c8a880b87626
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- path: ./output/bowtie2/bowtie2/genome.rev.1.bt2l
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md5sum: fda48e35925fb24d1c0785f021981e25
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- path: ./output/bowtie2/bowtie2/genome.rev.2.bt2l
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md5sum: 802c26d32b970e1b105032b7ce7348b4
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@ -9,15 +9,14 @@ workflow test_vardictjava {
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bam_input_ch = Channel.value([
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[ id:'test' ], // meta map
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file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
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file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
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file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true),
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file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true)
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])
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bed = Channel.value(file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true))
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reference = Channel.value([
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file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true),
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file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true)
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])
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VARDICTJAVA ( bam_input_ch, bed, reference )
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VARDICTJAVA ( bam_input_ch, reference )
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}
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