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Creation of Glimpse Chunk (#1985)
* Creation of Glimpse Chunk Add sub tools chunk with configuration and needed files for unitest. * Code linting correction * Prettier ran Newline deleted in nextflow.config * New final line and white spaces * Change Pytest.yml to work as a subtool. * Control for the parameters deleted. Control for the size/count of the window and buffer not needed. They have some defaults value. * Changed the different recommended part by @nvnieuwk Co-authored-by: nvnieuwk <101190534+nvnieuwk@users.noreply.github.com>
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39
modules/glimpse/chunk/main.nf
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39
modules/glimpse/chunk/main.nf
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process GLIMPSE_CHUNK {
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tag "$meta.id"
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label 'process_medium'
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conda (params.enable_conda ? "bioconda::glimpse-bio=1.1.1" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/glimpse-bio:1.1.1--h2ce4488_2':
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'quay.io/biocontainers/glimpse-bio:1.1.1--hce55b13_1' }"
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input:
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tuple val(meta), path(input)
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val region
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output:
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tuple val(meta), path("*.txt"), emit: chunk_chr
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def prefix = task.ext.prefix ?: "${meta.id}"
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def args = task.ext.args ?: ""
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def VERSION = '1.1.1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
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"""
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GLIMPSE_chunk \\
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$args \\
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--input $input \\
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--region $region \\
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--thread $task.cpus \\
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--output ${prefix}.txt
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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glimpse: $VERSION
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END_VERSIONS
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"""
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}
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52
modules/glimpse/chunk/meta.yml
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modules/glimpse/chunk/meta.yml
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name: "glimpse_chunk"
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description: Defines chunks where to run imputation
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keywords:
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- chunk
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- imputation
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tools:
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- "glimpse":
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description: "GLIMPSE is a phasing and imputation method for large-scale low-coverage sequencing studies."
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homepage: "https://odelaneau.github.io/GLIMPSE"
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documentation: "https://odelaneau.github.io/GLIMPSE/commands.html"
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tool_dev_url: "https://github.com/odelaneau/GLIMPSE"
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doi: "10.1038/s41588-020-00756-0"
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licence: "['MIT']"
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input:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- input:
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type: file
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description: |
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Target dataset in VCF/BCF format defined at all variable positions.
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The file could possibly be without GT field (for efficiency reasons a file containing only the positions is recommended).
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pattern: "*.{vcf,bcf}"
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- region:
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type: string
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description: |
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Target region, usually a full chromosome (e.g. chr20:1000000-2000000 or chr20).
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For chrX, please treat PAR and non-PAR regions as different choromosome in order to avoid mixing ploidy.
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output:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- versions:
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type: file
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description: File containing software versions
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pattern: "versions.yml"
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- txt:
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type: file
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description: Tab delimited output txt file containing buffer and imputation regions.
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pattern: "*.{txt}"
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authors:
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- "@louislenezet"
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@ -1015,6 +1015,10 @@ gffread:
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- modules/gffread/**
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- modules/gffread/**
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- tests/modules/gffread/**
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- tests/modules/gffread/**
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glimpse/chunk:
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- modules/glimpse/chunk/**
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- tests/modules/glimpse/chunk/**
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glnexus:
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glnexus:
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- modules/glnexus/**
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- modules/glnexus/**
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- tests/modules/glnexus/**
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- tests/modules/glnexus/**
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14
tests/modules/glimpse/chunk/main.nf
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14
tests/modules/glimpse/chunk/main.nf
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#!/usr/bin/env nextflow
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nextflow.enable.dsl = 2
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include { GLIMPSE_CHUNK } from '../../../../modules/glimpse/chunk/main.nf'
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workflow test_glimpse_chunk {
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input = [
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[ id:'test', single_end:false ], // meta map
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[file(params.test_data['homo_sapiens']['genome']['mills_and_1000g_indels_21_vcf_gz'], checkIfExists: true),
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file(params.test_data['homo_sapiens']['genome']['mills_and_1000g_indels_21_vcf_gz_tbi'], checkIfExists: true)]
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]
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GLIMPSE_CHUNK (input,"chr21")
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}
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10
tests/modules/glimpse/chunk/nextflow.config
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tests/modules/glimpse/chunk/nextflow.config
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process {
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withName: glimpse_chunk {
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ext.args = [
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"--window-size 2000000",
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"--buffer-size 200000"
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].join(' ')
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ext.prefix = { "${meta.id}" }
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}
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publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
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}
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8
tests/modules/glimpse/chunk/test.yml
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8
tests/modules/glimpse/chunk/test.yml
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- name: glimpse chunk test_glimpse_chunk
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command: nextflow run ./tests/modules/glimpse/chunk -entry test_glimpse_chunk -c ./tests/config/nextflow.config -c ./tests/modules/glimpse/chunk/nextflow.config
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tags:
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- glimpse
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- glimpse/chunk
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files:
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- path: output/glimpse/test.txt
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md5sum: c690cdcc7aa086bb85431bb2b193176f
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