created the module

This commit is contained in:
Nicolas Vannieuwkerke 2022-04-27 11:12:58 +02:00
parent 6a46e7cf11
commit 024c992ca7
6 changed files with 167 additions and 0 deletions

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// TODO nf-core: If in doubt look at other nf-core/modules to see how we are doing things! :)
// https://github.com/nf-core/modules/tree/master/modules
// You can also ask for help via your pull request or on the #modules channel on the nf-core Slack workspace:
// https://nf-co.re/join
// TODO nf-core: A module file SHOULD only define input and output files as command-line parameters.
// All other parameters MUST be provided using the "task.ext" directive, see here:
// https://www.nextflow.io/docs/latest/process.html#ext
// where "task.ext" is a string.
// Any parameters that need to be evaluated in the context of a particular sample
// e.g. single-end/paired-end data MUST also be defined and evaluated appropriately.
// TODO nf-core: Software that can be piped together SHOULD be added to separate module files
// unless there is a run-time, storage advantage in implementing in this way
// e.g. it's ok to have a single module for bwa to output BAM instead of SAM:
// bwa mem | samtools view -B -T ref.fasta
// TODO nf-core: Optional inputs are not currently supported by Nextflow. However, using an empty
// list (`[]`) instead of a file can be used to work around this issue.
process VARDICTJAVA {
tag "$meta.id"
label 'process_medium'
// TODO nf-core: List required Conda package(s).
// Software MUST be pinned to channel (i.e. "bioconda"), version (i.e. "1.10").
// For Conda, the build (i.e. "h9402c20_2") must be EXCLUDED to support installation on different operating systems.
// TODO nf-core: See section in main README for further information regarding finding and adding container addresses to the section below.
conda (params.enable_conda ? "bioconda::vardict-java=1.8.3" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/vardict-java:1.8.3--hdfd78af_0':
'quay.io/biocontainers/vardict-java:1.8.3--hdfd78af_0' }"
input:
// TODO nf-core: Where applicable all sample-specific information e.g. "id", "single_end", "read_group"
// MUST be provided as an input via a Groovy Map called "meta".
// This information may not be required in some instances e.g. indexing reference genome files:
// https://github.com/nf-core/modules/blob/master/modules/bwa/index/main.nf
// TODO nf-core: Where applicable please provide/convert compressed files as input/output
// e.g. "*.fastq.gz" and NOT "*.fastq", "*.bam" and NOT "*.sam" etc.
tuple val(meta), path(bam)
path(reference_fasta)
path(regions_of_interest)
output:
// TODO nf-core: Named file extensions MUST be emitted for ALL output channels
tuple val(meta), path("*.bam"), emit: bam
// TODO nf-core: List additional required output channels/values here
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
// TODO nf-core: Where possible, a command MUST be provided to obtain the version number of the software e.g. 1.10
// If the software is unable to output a version number on the command-line then it can be manually specified
// e.g. https://github.com/nf-core/modules/blob/master/modules/homer/annotatepeaks/main.nf
// Each software used MUST provide the software name and version number in the YAML version file (versions.yml)
// TODO nf-core: It MUST be possible to pass additional parameters to the tool as a command-line string via the "task.ext.args" directive
// TODO nf-core: If the tool supports multi-threading then you MUST provide the appropriate parameter
// using the Nextflow "task" variable e.g. "--threads $task.cpus"
// TODO nf-core: Please replace the example samtools command below with your module's command
// TODO nf-core: Please indent the command appropriately (4 spaces!!) to help with readability ;)
"""
vardict-java \\
$args \\
-b $bam \\
-th $task.cpus \\
-n $prefix \\
-G $reference_fasta \\
$regions_of_interest \\
cat <<-END_VERSIONS > versions.yml
"${task.process}":
vardictjava: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' ))
END_VERSIONS
"""
}

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name: "vardictjava"
## TODO nf-core: Add a description of the module and list keywords
description: write your description here
keywords:
- sort
tools:
- "vardictjava":
## TODO nf-core: Add a description and other details for the software below
description: "Java port of the VarDict variant discovery program"
homepage: "None"
documentation: "None"
tool_dev_url: "None"
doi: ""
licence: "['MIT']"
## TODO nf-core: Add a description of all of the variables used as input
input:
# Only when we have meta
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
#
## TODO nf-core: Delete / customise this example input
- bam:
type: file
description: BAM/CRAM/SAM file
pattern: "*.{bam,cram,sam}"
## TODO nf-core: Add a description of all of the variables used as output
output:
#Only when we have meta
- meta:
type: map
description: |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
#
- versions:
type: file
description: File containing software versions
pattern: "versions.yml"
## TODO nf-core: Delete / customise this example output
- bam:
type: file
description: Sorted BAM/CRAM/SAM file
pattern: "*.{bam,cram,sam}"
authors:
- "@nvnieuwk"

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- modules/unzip/** - modules/unzip/**
- tests/modules/unzip/** - tests/modules/unzip/**
vardictjava:
- modules/vardictjava/**
- tests/modules/vardictjava/**
variantbam: variantbam:
- modules/variantbam/** - modules/variantbam/**
- tests/modules/variantbam/** - tests/modules/variantbam/**

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#!/usr/bin/env nextflow
nextflow.enable.dsl = 2
include { VARDICTJAVA } from '../../../modules/vardictjava/main.nf'
workflow test_vardictjava {
input = [
[ id:'test', single_end:false ], // meta map
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_bam'], checkIfExists: true)
]
VARDICTJAVA ( input )
}

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process {
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
withName: VARDICTJAVA {
ext.args = ''
}
}

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## TODO nf-core: Please run the following command to build this file:
# nf-core modules create-test-yml vardictjava
- name: "vardictjava"
command: nextflow run ./tests/modules/vardictjava -entry test_vardictjava -c ./tests/config/nextflow.config -c ./tests/modules/vardictjava/nextflow.config
tags:
- "vardictjava"
#
files:
- path: "output/vardictjava/test.bam"
md5sum: e667c7caad0bc4b7ac383fd023c654fc
- path: output/vardictjava/versions.yml
md5sum: a01fe51bc4c6a3a6226fbf77b2c7cf3b