diff --git a/modules/gatk4/mergebamalignment/main.nf b/modules/gatk4/mergebamalignment/main.nf index 7ba9ccda..5c36b4ba 100644 --- a/modules/gatk4/mergebamalignment/main.nf +++ b/modules/gatk4/mergebamalignment/main.nf @@ -43,4 +43,15 @@ process GATK4_MERGEBAMALIGNMENT { gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ } diff --git a/modules/gatk4/mutect2/main.nf b/modules/gatk4/mutect2/main.nf index 4a1f5768..9969ad70 100644 --- a/modules/gatk4/mutect2/main.nf +++ b/modules/gatk4/mutect2/main.nf @@ -57,4 +57,18 @@ process GATK4_MUTECT2 { gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.vcf.gz + touch ${prefix}.vcf.gz.tbi + touch ${prefix}.vcf.gz.stats + touch ${prefix}.f1r2.tar.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ } diff --git a/modules/gatk4/revertsam/main.nf b/modules/gatk4/revertsam/main.nf index 4e8e9ddc..3084658d 100644 --- a/modules/gatk4/revertsam/main.nf +++ b/modules/gatk4/revertsam/main.nf @@ -39,4 +39,15 @@ process GATK4_REVERTSAM { gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.reverted.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ } diff --git a/modules/gatk4/samtofastq/main.nf b/modules/gatk4/samtofastq/main.nf index 8553e419..d8d94d69 100644 --- a/modules/gatk4/samtofastq/main.nf +++ b/modules/gatk4/samtofastq/main.nf @@ -40,4 +40,17 @@ process GATK4_SAMTOFASTQ { gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.fastq.gz + touch ${prefix}_1.fastq.gz + touch ${prefix}_2.fastq.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') + END_VERSIONS + """ } diff --git a/modules/samtools/view/main.nf b/modules/samtools/view/main.nf index 11cfb74b..55194e88 100644 --- a/modules/samtools/view/main.nf +++ b/modules/samtools/view/main.nf @@ -41,4 +41,16 @@ process SAMTOOLS_VIEW { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bam + touch ${prefix}.cram + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ } diff --git a/modules/shigatyper/main.nf b/modules/shigatyper/main.nf new file mode 100644 index 00000000..9d5c189a --- /dev/null +++ b/modules/shigatyper/main.nf @@ -0,0 +1,64 @@ +process SHIGATYPER { + tag "$meta.id" + label 'process_low' + + conda (params.enable_conda ? "bioconda::shigatyper=2.0.1" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/shigatyper%3A2.0.1--pyhdfd78af_0': + 'quay.io/biocontainers/shigatyper:2.0.1--pyhdfd78af_0' }" + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("${prefix}.tsv") , emit: tsv + tuple val(meta), path("${prefix}-hits.tsv"), optional: true, emit: hits + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + + if (meta.is_ont) { + """ + shigatyper \\ + $args \\ + --SE $reads \\ + --ont \\ + --name $prefix + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + shigatyper: \$(echo \$(shigatyper --version 2>&1) | sed 's/^.*ShigaTyper //' ) + END_VERSIONS + """ + } else if (meta.single_end) { + """ + shigatyper \\ + $args \\ + --SE $reads \\ + --name $prefix + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + shigatyper: \$(echo \$(shigatyper --version 2>&1) | sed 's/^.*ShigaTyper //' ) + END_VERSIONS + """ + } else { + """ + shigatyper \\ + $args \\ + --R1 ${reads[0]} \\ + --R2 ${reads[1]} \\ + --name $prefix + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + shigatyper: \$(echo \$(shigatyper --version 2>&1) | sed 's/^.*ShigaTyper //' ) + END_VERSIONS + """ + } +} diff --git a/modules/shigatyper/meta.yml b/modules/shigatyper/meta.yml new file mode 100644 index 00000000..ebaded6b --- /dev/null +++ b/modules/shigatyper/meta.yml @@ -0,0 +1,47 @@ +name: "shigatyper" +description: Determine Shigella serotype from Illumina or Oxford Nanopore reads +keywords: + - fastq + - shigella + - serotype +tools: + - "shigatyper": + description: "Typing tool for Shigella spp. from WGS Illumina sequencing" + homepage: "https://github.com/CFSAN-Biostatistics/shigatyper" + documentation: "https://github.com/CFSAN-Biostatistics/shigatyper" + tool_dev_url: "https://github.com/CFSAN-Biostatistics/shigatyper" + doi: "10.1128/AEM.00165-19" + licence: "['Public Domain']" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false, is_ont:false ] + - reads: + type: file + description: Illumina or Nanopore FASTQ file + pattern: "*.fastq.gz" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - tsv: + type: file + description: A TSV formatted file with ShigaTyper results + pattern: "*.tsv" + - hits: + type: file + description: A TSV formatted file with individual gene hits + pattern: "*-hits.tsv" + +authors: + - "@rpetit3" diff --git a/modules/slimfastq/main.nf b/modules/slimfastq/main.nf new file mode 100644 index 00000000..19ca876f --- /dev/null +++ b/modules/slimfastq/main.nf @@ -0,0 +1,52 @@ +def VERSION = '2.04' + +process SLIMFASTQ { + tag "$meta.id" + label 'process_low' + + conda (params.enable_conda ? "bioconda::slimfastq=2.04" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/slimfastq:2.04--h87f3376_2': + 'quay.io/biocontainers/slimfastq:2.04--h87f3376_2' }" + + input: + tuple val(meta), path(fastq) + + output: + tuple val(meta), path("*.sfq"), emit: sfq + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + if (meta.single_end) { + """ + gzip -d -c '${fastq}' | slimfastq \\ + $args \\ + -f '${prefix}.sfq' + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + slimfastq: ${VERSION} + END_VERSIONS + """ + } else { + """ + gzip -d -c '${fastq[0]}' | slimfastq \\ + $args \\ + -f '${prefix}_1.sfq' + + gzip -d -c '${fastq[1]}' | slimfastq \\ + $args \\ + -f '${prefix}_2.sfq' + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + slimfastq: ${VERSION} + END_VERSIONS + """ + } +} diff --git a/modules/slimfastq/meta.yml b/modules/slimfastq/meta.yml new file mode 100644 index 00000000..3b040f25 --- /dev/null +++ b/modules/slimfastq/meta.yml @@ -0,0 +1,41 @@ +name: "slimfastq" +description: Fast, efficient, lossless compression of FASTQ files. +keywords: + - FASTQ + - compression + - lossless +tools: + - "slimfastq": + description: "slimfastq efficiently compresses/decompresses FASTQ files" + homepage: "https://github.com/Infinidat/slimfastq" + tool_dev_url: "https://github.com/Infinidat/slimfastq" + licence: "['BSD-3-clause']" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - fastq: + type: file + description: Either a single-end FASTQ file or paired-end files. + pattern: "*.{fq.gz,fastq.gz}" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - sfq: + type: file + description: Either one or two sequence files in slimfastq compressed format. + pattern: "*.{sfq}" + +authors: + - "@Midnighter" diff --git a/modules/vardictjava/main.nf b/modules/vardictjava/main.nf new file mode 100644 index 00000000..454b86a4 --- /dev/null +++ b/modules/vardictjava/main.nf @@ -0,0 +1,50 @@ +def VERSION = '1.8.3' + +process VARDICTJAVA { + tag "$meta.id" + label 'process_medium' + + conda (params.enable_conda ? "bioconda::vardict-java=1.8.3" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/vardict-java:1.8.3--hdfd78af_0': + 'quay.io/biocontainers/vardict-java:1.8.3--hdfd78af_0' }" + + input: + tuple val(meta), path(bam), path(bai) + path(bed) + tuple path(fasta), path(fasta_fai) + + output: + tuple val(meta), path("*.vcf.gz"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + vardict-java \\ + $args \\ + -c 1 -S 2 -E 3 \\ + -b $bam \\ + -th $task.cpus \\ + -N $prefix \\ + -G $fasta \\ + $bed \\ + | teststrandbias.R \\ + | var2vcf_valid.pl \\ + $args2 \\ + -N $prefix \\ + | gzip -c > ${prefix}.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + vardict-java: $VERSION + var2vcf_valid.pl: \$(echo \$(var2vcf_valid.pl -h | sed -n 2p | awk '{ print \$2 }')) + END_VERSIONS + """ +} diff --git a/modules/vardictjava/meta.yml b/modules/vardictjava/meta.yml new file mode 100644 index 00000000..59fba966 --- /dev/null +++ b/modules/vardictjava/meta.yml @@ -0,0 +1,60 @@ +name: "vardictjava" + +description: The Java port of the VarDict variant caller +keywords: + - variant calling + - VarDict + - AstraZeneca +tools: + - "vardictjava": + description: "Java port of the VarDict variant discovery program" + homepage: "https://github.com/AstraZeneca-NGS/VarDictJava" + documentation: "https://github.com/AstraZeneca-NGS/VarDictJava" + tool_dev_url: "https://github.com/AstraZeneca-NGS/VarDictJava" + doi: "10.1093/nar/gkw227 " + licence: "['MIT']" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/SAM file + pattern: "*.{bam,sam}" + - bai: + type: file + description: Index of the BAM file + pattern: "*.bai" + - fasta: + type: file + description: FASTA of the reference genome + pattern: "*.{fa,fasta}" + - fasta_fai: + type: file + description: The index of the FASTA of the reference genome + pattern: "*.fai" + - bed: + type: file + description: BED with the regions of interest + pattern: "*.bed" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: VCF file output + pattern: "*.vcf.gz" + +authors: + - "@nvnieuwk" diff --git a/tests/config/pytest_modules.yml b/tests/config/pytest_modules.yml index 924447d3..f4feb844 100644 --- a/tests/config/pytest_modules.yml +++ b/tests/config/pytest_modules.yml @@ -1719,6 +1719,10 @@ seqwish/induce: - modules/seqwish/induce/** - tests/modules/seqwish/induce/** +shigatyper: + - modules/shigatyper/** + - tests/modules/shigatyper/** + shovill: - modules/shovill/** - tests/modules/shovill/** @@ -1727,6 +1731,10 @@ sistr: - modules/sistr/** - tests/modules/sistr/** +slimfastq: + - modules/slimfastq/** + - tests/modules/slimfastq/** + snapaligner/index: - modules/snapaligner/index/** - tests/modules/snapaligner/index/** @@ -1920,6 +1928,10 @@ unzip: - modules/unzip/** - tests/modules/unzip/** +vardictjava: + - modules/vardictjava/** + - tests/modules/vardictjava/** + variantbam: - modules/variantbam/** - tests/modules/variantbam/** diff --git a/tests/modules/gatk4/mergebamalignment/main.nf b/tests/modules/gatk4/mergebamalignment/main.nf index 8a38c129..0eb6876d 100644 --- a/tests/modules/gatk4/mergebamalignment/main.nf +++ b/tests/modules/gatk4/mergebamalignment/main.nf @@ -14,3 +14,14 @@ workflow test_gatk4_mergebamalignment { GATK4_MERGEBAMALIGNMENT ( input, fasta, dict ) } + +workflow test_gatk4_mergebamalignment_stubs { + input = [ [ id:'test' ], // meta map + "test_foo.bam", + "test_bar.bam" + ] + fasta = "genome.fasta" + dict = "genome.fasta.dict" + + GATK4_MERGEBAMALIGNMENT ( input, fasta, dict ) +} diff --git a/tests/modules/gatk4/mergebamalignment/test.yml b/tests/modules/gatk4/mergebamalignment/test.yml index b1bb32b2..84a67654 100644 --- a/tests/modules/gatk4/mergebamalignment/test.yml +++ b/tests/modules/gatk4/mergebamalignment/test.yml @@ -7,3 +7,12 @@ - path: output/gatk4/test.bam md5sum: e6f1b343700b7ccb94e81ae127433988 - path: output/gatk4/versions.yml + +- name: gatk4 mergebamalignment test_gatk4_mergebamalignment_stubs + command: nextflow run ./tests/modules/gatk4/mergebamalignment -entry test_gatk4_mergebamalignment -c ./tests/config/nextflow.config -c ./tests/modules/gatk4/mergebamalignment/nextflow.config -stub-run + tags: + - gatk4 + - gatk4/mergebamalignment + files: + - path: output/gatk4/test.bam + - path: output/gatk4/versions.yml diff --git a/tests/modules/gatk4/mutect2/main.nf b/tests/modules/gatk4/mutect2/main.nf index 0b4339f0..310e9ca1 100644 --- a/tests/modules/gatk4/mutect2/main.nf +++ b/tests/modules/gatk4/mutect2/main.nf @@ -118,3 +118,25 @@ workflow test_gatk4_mutect2_mitochondria { GATK4_MUTECT2_MITO ( input, fasta, fai, dict, [], [], [], [] ) } + +workflow test_gatk4_mutect2_tumor_normal_pair_f1r2_stubs { + input = [ [ id:'test', normal_id:'normal', tumor_id:'tumour' ], // meta map + [ "foo_paired.bam", + "foo_paired2.bam" + ], + [ "foo_paired.bam.bai", + "foo_paired2.bam.bai" + ], + [] + ] + + fasta = "genome.fasta" + fai = "genome.fasta.fai" + dict = "genome.fasta.dict" + germline_resource = "genome_gnomAD.r2.1.1.vcf.gz" + germline_resource_tbi = "genome_gnomAD.r2.1.1.vcf.gz.tbi" + panel_of_normals = "genome_mills_and_1000G.indels.hg38.vcf.gz" + panel_of_normals_tbi = "genome_mills_and_1000G.indels.hg38.vcf.gz.tbi" + + GATK4_MUTECT2_F1R2 ( input, fasta, fai, dict, germline_resource, germline_resource_tbi, panel_of_normals, panel_of_normals_tbi ) +} diff --git a/tests/modules/gatk4/mutect2/test.yml b/tests/modules/gatk4/mutect2/test.yml index 3cefce09..3853801d 100644 --- a/tests/modules/gatk4/mutect2/test.yml +++ b/tests/modules/gatk4/mutect2/test.yml @@ -69,3 +69,15 @@ md5sum: fc6ea14ca2da346babe78161beea28c9 - path: output/gatk4/test.vcf.gz.tbi - path: output/gatk4/versions.yml + +- name: gatk4 mutect2 test_gatk4_mutect2_tumor_normal_pair_f1r2_stubs + command: nextflow run ./tests/modules/gatk4/mutect2 -entry test_gatk4_mutect2_tumor_normal_pair_f1r2 -c ./tests/config/nextflow.config -c ./tests/modules/gatk4/mutect2/nextflow.config -stub-run + tags: + - gatk4 + - gatk4/mutect2 + files: + - path: output/gatk4/test.f1r2.tar.gz + - path: output/gatk4/test.vcf.gz + - path: output/gatk4/test.vcf.gz.stats + - path: output/gatk4/test.vcf.gz.tbi + - path: output/gatk4/versions.yml diff --git a/tests/modules/gatk4/revertsam/main.nf b/tests/modules/gatk4/revertsam/main.nf index ab5dddee..738ecd8f 100644 --- a/tests/modules/gatk4/revertsam/main.nf +++ b/tests/modules/gatk4/revertsam/main.nf @@ -11,3 +11,11 @@ workflow test_gatk4_revertsam { GATK4_REVERTSAM ( input ) } + +workflow test_gatk4_revertsam_stubs { + input = [ [ id:'test' ], // meta map + "foo_paired_end.bam" + ] + + GATK4_REVERTSAM ( input ) +} diff --git a/tests/modules/gatk4/revertsam/test.yml b/tests/modules/gatk4/revertsam/test.yml index 2ebdb685..89e78659 100644 --- a/tests/modules/gatk4/revertsam/test.yml +++ b/tests/modules/gatk4/revertsam/test.yml @@ -7,3 +7,12 @@ - path: output/gatk4/test.reverted.bam md5sum: f783a88deb45c3a2c20ca12cbe1c5652 - path: output/gatk4/versions.yml + +- name: gatk4 revertsam test_gatk4_revertsam_stubs + command: nextflow run ./tests/modules/gatk4/revertsam -entry test_gatk4_revertsam -c ./tests/config/nextflow.config -c ./tests/modules/gatk4/revertsam/nextflow.config -stub-run + tags: + - gatk4 + - gatk4/revertsam + files: + - path: output/gatk4/test.reverted.bam + - path: output/gatk4/versions.yml diff --git a/tests/modules/gatk4/samtofastq/main.nf b/tests/modules/gatk4/samtofastq/main.nf index 26a8ce2d..79d04c7c 100644 --- a/tests/modules/gatk4/samtofastq/main.nf +++ b/tests/modules/gatk4/samtofastq/main.nf @@ -19,3 +19,11 @@ workflow test_gatk4_samtofastq_paired_end { GATK4_SAMTOFASTQ ( input ) } + +workflow test_gatk4_samtofastq_paired_end_stubs { + input = [ [ id:'test', single_end: false ], // meta map + [ "foo_paired_end.bam" ] + ] + + GATK4_SAMTOFASTQ ( input ) +} diff --git a/tests/modules/gatk4/samtofastq/test.yml b/tests/modules/gatk4/samtofastq/test.yml index eb25f33b..1288a270 100644 --- a/tests/modules/gatk4/samtofastq/test.yml +++ b/tests/modules/gatk4/samtofastq/test.yml @@ -19,3 +19,13 @@ - path: output/gatk4/test_2.fastq.gz md5sum: 613bf64c023609e1c62ad6ce9e4be8d7 - path: output/gatk4/versions.yml + +- name: gatk4 samtofastq test_gatk4_samtofastq_paired_end_stubs + command: nextflow run ./tests/modules/gatk4/samtofastq -entry test_gatk4_samtofastq_paired_end -c ./tests/config/nextflow.config -c ./tests/modules/gatk4/samtofastq/nextflow.config -stub-run + tags: + - gatk4 + - gatk4/samtofastq + files: + - path: output/gatk4/test_1.fastq.gz + - path: output/gatk4/test_2.fastq.gz + - path: output/gatk4/versions.yml diff --git a/tests/modules/samtools/view/main.nf b/tests/modules/samtools/view/main.nf index 9c239066..bdad1078 100644 --- a/tests/modules/samtools/view/main.nf +++ b/tests/modules/samtools/view/main.nf @@ -22,3 +22,12 @@ workflow test_samtools_view_cram { SAMTOOLS_VIEW ( input, fasta ) } + +workflow test_samtools_view_stubs { + input = [ [ id:'test', single_end:false ], // meta map + "foo_paired_end.bam", + [] + ] + + SAMTOOLS_VIEW ( input, [] ) +} diff --git a/tests/modules/samtools/view/test.yml b/tests/modules/samtools/view/test.yml index 1287d455..2718130e 100644 --- a/tests/modules/samtools/view/test.yml +++ b/tests/modules/samtools/view/test.yml @@ -14,3 +14,11 @@ - samtools files: - path: output/samtools/test.cram + +- name: samtools view test_samtools_view_stubs + command: nextflow run ./tests/modules/samtools/view -entry test_samtools_view -c ./tests/config/nextflow.config -c ./tests/modules/samtools/view/nextflow.config -stub-run + tags: + - samtools/view + - samtools + files: + - path: output/samtools/test.bam diff --git a/tests/modules/shigatyper/main.nf b/tests/modules/shigatyper/main.nf new file mode 100644 index 00000000..eb4df084 --- /dev/null +++ b/tests/modules/shigatyper/main.nf @@ -0,0 +1,36 @@ +#!/usr/bin/env nextflow + +nextflow.enable.dsl = 2 + +include { SHIGATYPER } from '../../../modules/shigatyper/main.nf' + +workflow test_shigatyper_pe { + + input = [ + [ id:'test', single_end:false, is_ont:false ], // meta map + [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] + ] + + SHIGATYPER ( input ) +} + +workflow test_shigatyper_se { + + input = [ + [ id:'test', single_end:true, is_ont:false ], // meta map + [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] + ] + + SHIGATYPER ( input ) +} + +workflow test_shigatyper_ont { + + input = [ + [ id:'test', single_end:true, is_ont:true ], // meta map + [ file(params.test_data['sarscov2']['nanopore']['test_fastq_gz'], checkIfExists: true) ] + ] + + SHIGATYPER ( input ) +} diff --git a/tests/modules/shigatyper/nextflow.config b/tests/modules/shigatyper/nextflow.config new file mode 100644 index 00000000..50f50a7a --- /dev/null +++ b/tests/modules/shigatyper/nextflow.config @@ -0,0 +1,5 @@ +process { + + publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" } + +} \ No newline at end of file diff --git a/tests/modules/shigatyper/test.yml b/tests/modules/shigatyper/test.yml new file mode 100644 index 00000000..9dda573b --- /dev/null +++ b/tests/modules/shigatyper/test.yml @@ -0,0 +1,29 @@ +- name: shigatyper test_shigatyper_pe + command: nextflow run tests/modules/shigatyper -entry test_shigatyper_pe -c tests/config/nextflow.config -c tests/modules/shigatyper/nextflow.config + tags: + - shigatyper + files: + - path: output/shigatyper/test.tsv + md5sum: 4f7d38c956993800546b9acb9881d717 + - path: output/shigatyper/versions.yml + md5sum: d8ca45ed88dfba9bc570c01e4b49773b + +- name: shigatyper test_shigatyper_se + command: nextflow run tests/modules/shigatyper -entry test_shigatyper_se -c tests/config/nextflow.config -c tests/modules/shigatyper/nextflow.config + tags: + - shigatyper + files: + - path: output/shigatyper/test.tsv + md5sum: 4f7d38c956993800546b9acb9881d717 + - path: output/shigatyper/versions.yml + md5sum: 8bbf165da5a5df3b7771a33aad197eec + +- name: shigatyper test_shigatyper_ont + command: nextflow run tests/modules/shigatyper -entry test_shigatyper_ont -c tests/config/nextflow.config -c tests/modules/shigatyper/nextflow.config + tags: + - shigatyper + files: + - path: output/shigatyper/test.tsv + md5sum: 4f7d38c956993800546b9acb9881d717 + - path: output/shigatyper/versions.yml + md5sum: 0da333e1178e9e7e84a9116ad5a5ff71 diff --git a/tests/modules/slimfastq/main.nf b/tests/modules/slimfastq/main.nf new file mode 100644 index 00000000..40d44285 --- /dev/null +++ b/tests/modules/slimfastq/main.nf @@ -0,0 +1,46 @@ +#!/usr/bin/env nextflow + +nextflow.enable.dsl = 2 + +include { SLIMFASTQ } from '../../../modules/slimfastq/main.nf' + +workflow test_slimfastq_single_end { + + input = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + + SLIMFASTQ ( input ) +} + +workflow test_slimfastq_paired_end { + + input = [ + [ id:'test', single_end:false ], // meta map + [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true)] + ] + + SLIMFASTQ ( input ) +} + +workflow test_slimfastq_nanopore { + + input = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['nanopore']['test_fastq_gz'], checkIfExists: true) + ] + + SLIMFASTQ ( input ) +} + +workflow test_slimfastq_pacbio { + + input = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['homo_sapiens']['pacbio']['ccs_fq_gz'], checkIfExists: true) + ] + + SLIMFASTQ ( input ) +} diff --git a/tests/modules/slimfastq/nextflow.config b/tests/modules/slimfastq/nextflow.config new file mode 100644 index 00000000..50f50a7a --- /dev/null +++ b/tests/modules/slimfastq/nextflow.config @@ -0,0 +1,5 @@ +process { + + publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" } + +} \ No newline at end of file diff --git a/tests/modules/slimfastq/test.yml b/tests/modules/slimfastq/test.yml new file mode 100644 index 00000000..f0d8b446 --- /dev/null +++ b/tests/modules/slimfastq/test.yml @@ -0,0 +1,41 @@ +- name: slimfastq test_slimfastq_single_end + command: nextflow run tests/modules/slimfastq -entry test_slimfastq_single_end -c tests/config/nextflow.config + tags: + - slimfastq + files: + - path: output/slimfastq/test.sfq + md5sum: 6a942eeca6c99ee9a9a0cedab5d246f1 + - path: output/slimfastq/versions.yml + md5sum: f52351f5c9e6259af02745c8eae5c780 + +- name: slimfastq test_slimfastq_paired_end + command: nextflow run tests/modules/slimfastq -entry test_slimfastq_paired_end -c tests/config/nextflow.config + tags: + - slimfastq + files: + - path: output/slimfastq/test_1.sfq + md5sum: 6a942eeca6c99ee9a9a0cedab5d246f1 + - path: output/slimfastq/test_2.sfq + md5sum: 0d2c60b52a39f7c2cb7843e848d90afd + - path: output/slimfastq/versions.yml + md5sum: 6239853705877651a4851c4cb6d62da4 + +- name: slimfastq test_slimfastq_nanopore + command: nextflow run tests/modules/slimfastq -entry test_slimfastq_nanopore -c tests/config/nextflow.config + tags: + - slimfastq + files: + - path: output/slimfastq/test.sfq + md5sum: e17f14d64d3a75356b03ff2f9e8881f7 + - path: output/slimfastq/versions.yml + md5sum: 33153f1103482a2bd35cb2f4c337c5e8 + +- name: slimfastq test_slimfastq_pacbio + command: nextflow run tests/modules/slimfastq -entry test_slimfastq_pacbio -c tests/config/nextflow.config + tags: + - slimfastq + files: + - path: output/slimfastq/test.sfq + md5sum: 9e8389e47e6ddf8c25e92412dd628339 + - path: output/slimfastq/versions.yml + md5sum: 1982789c3d5c7de37c0a9351e4ae63f7 diff --git a/tests/modules/vardictjava/main.nf b/tests/modules/vardictjava/main.nf new file mode 100644 index 00000000..d392358a --- /dev/null +++ b/tests/modules/vardictjava/main.nf @@ -0,0 +1,23 @@ +#!/usr/bin/env nextflow + +nextflow.enable.dsl = 2 + +include { VARDICTJAVA } from '../../../modules/vardictjava/main.nf' + +workflow test_vardictjava { + + bam_input_ch = Channel.value([ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true) + ]) + + bed = Channel.value(file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true)) + + reference = Channel.value([ + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true), + file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) + ]) + + VARDICTJAVA ( bam_input_ch, bed, reference ) +} diff --git a/tests/modules/vardictjava/nextflow.config b/tests/modules/vardictjava/nextflow.config new file mode 100644 index 00000000..50f50a7a --- /dev/null +++ b/tests/modules/vardictjava/nextflow.config @@ -0,0 +1,5 @@ +process { + + publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" } + +} \ No newline at end of file diff --git a/tests/modules/vardictjava/test.yml b/tests/modules/vardictjava/test.yml new file mode 100644 index 00000000..8cb29c4e --- /dev/null +++ b/tests/modules/vardictjava/test.yml @@ -0,0 +1,9 @@ +- name: vardictjava test_vardictjava + command: nextflow run tests/modules/vardictjava -entry test_vardictjava -c tests/config/nextflow.config + tags: + - vardictjava + files: + - path: output/vardictjava/test.vcf.gz + md5sum: 3f1f227afc532bddeb58f16fd3013fc8 + - path: output/vardictjava/versions.yml + md5sum: 9b62c431a4f2680412b61c7071bdb1cd